Leucine-containing glyprolines (Pro-Gly-Pro-Leu and Leu-Pro-Gly-Pro): Participation in hemostatic reactions in vitro and in vivo in rats with blood coagulation and lipid metabolism disorders

Myasoedov, N. F.; Rochev, D. L.; Lyapina, L. A.; Оберган, Т.Ю.; Андреева, Л. А.

doi:10.1134/s0012496613060124

Cited by 4 publications

(6 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They are able to exhibit neuroprotective, antihypertensive, anti-inflammatory, and antithrombotic effects [ 7 , 40 ]. The multiple intranasal administration of PGP and PGPL to rats with hypercholesterolemia and increased blood clotting led to the normalization of cholesterol levels and the restoration of impaired functions of the anticoagulant system [ 24 , 25 , 26 ]. However, their contribution to gene expression during ischemia is not known for certain.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we used PGP and another synthetic PGP-containing peptide, Pro-Gly-Pro-Leu (PGPL), in the rat tMCAO model. It should be noted that the PGP and PGPL peptides have numerous effects, which may be beneficial for the treatment of cerebrovascular accidents [ 24 ]. The multiple intranasal administration of PGP and PGPL to rats with hypercholesterolemia and increased blood clotting led to the normalization of cholesterol levels and the restoration of impaired functions of the anticoagulant system [ 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…It should be noted that the PGP and PGPL peptides have numerous effects, which may be beneficial for the treatment of cerebrovascular accidents [ 24 ]. The multiple intranasal administration of PGP and PGPL to rats with hypercholesterolemia and increased blood clotting led to the normalization of cholesterol levels and the restoration of impaired functions of the anticoagulant system [ 24 , 25 , 26 ]. Under tMCAO conditions, we studied the effects of PGP and PGPL on the expression of a number of inflammatory cluster (IC) and neurotransmitter cluster (NC) genes in the rat brain and compared the effects of peptides with those of Semax using real-time RT-PCR.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Insight into Glyproline Peptides’ Activity through the Modulation of the Inflammatory and Neurosignaling Genetic Response Following Cerebral Ischemia–Reperfusion

Stavchansky

Filippenkov

Remizova

et al. 2022

Genes

View full text Add to dashboard Cite

Glyprolines are Gly-Pro (GP)- or Pro-Gly (PG)-containing biogenic peptides. These peptides can act as neutrophil chemoattractants, or atheroprotective, anticoagulant, and neuroprotective agents. The Pro-Gly-Pro (PGP) tripeptide is an active factor of resistance to the biodegradation of peptide drugs. The synthetic Semax peptide, which includes Met-Glu-His-Phe (MEHF) fragments of adrenocorticotropic hormone and the C-terminal tripeptide PGP, serves as a neuroprotective drug for the treatment of ischemic stroke. Previously, we revealed that Semax mostly prevented the disruption of the gene expression pattern 24 h after a transient middle cerebral artery occlusion (tMCAO) in a rat brain model. The genes of this pattern were grouped into an inflammatory cluster (IC) and a neurotransmitter cluster (NC). Here, using real-time RT-PCR, the effect of other PGP-containing peptides, PGP and Pro-Gly-Pro-Leu (PGPL), on the expression of a number of genes in the IC and NC was studied 24 h after tMCAO. Both the PGP and PGPL peptides showed Semax-unlike effects, predominantly without changing gene expression 24 h after tMCAO. Moreover, there were IC genes (iL1b, iL6, and Socs3) for PGP, as well as IC (iL6, Ccl3, Socs3, and Fos) and NC genes (Cplx2, Neurod6, and Ptk2b) for PGPL, that significantly changed in expression levels after peptide administration compared to Semax treatment under tMCAO conditions. Furthermore, gene enrichment analysis was carried out, and a regulatory gene network was constructed. Thus, the spectra of the common and unique effects of the PGP, PGPL, and Semax peptides under ischemia–reperfusion were distinguished.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Insight into Glyproline Peptides’ Activity through the Modulation of the Inflammatory and Neurosignaling Genetic Response Following Cerebral Ischemia–Reperfusion

Stavchansky

Filippenkov

Remizova

et al. 2022

Genes

View full text Add to dashboard Cite

show abstract

“…Было установлено, что пролин и глицинсодержащие пептиды PG и PGP могут индуцировать растворение нестабилизированного фибрина [16]. Наличие рецепторов глипролина на эндотелии пока не установлено, но сообщалось о существовании специфических сайтов связывания PGPL на цитоплазматической мембране базальных ядер головного мозга крыс [18]. Возможно, этот пептид, вводимый интраназальным путем, проходит через гематоэнцефалический барьер, проникая в структуры головного мозга и оказывая свое действие через специфические рецепторы.…”

Section: Discussionunclassified

Pharmacology complex compound of pro-gly-pro-leu with heparin: hypoglycemic, fibrinolitic and anticoagulant effects in rats with hyperglycemia

Оберган

Мясоедов

Григорьева

et al. 2019

Farm. farmakol. (Pâtigorsk)

Self Cite

View full text Add to dashboard Cite

Ранее показано, что применение регуляторных пептидов семейства глипролинов способствует нормализации системы гемостаза и уровня глюкозы крови при экспериментальной стойкой гипергликемии у крыс, подобной сахарному диабету 2 типа у человека. Также известно, что антикоагулянт гепарин подавляет свертывание крови и проявляет гипогликемическое действие в организме. Цель исследования. Получить комплекс пептида Pro-Gly-Pro-Leu (PGPL) и нефракционированного гепарина, изучить его влияние на уровень глюкозы и антикоагулянтно-фибринолитические свойства и показать его способность восстанавливать нарушенные функции инсулярной и свертывающей систем крови при экспериментальной гипергликемии у крыс. Материалы и методы. Использовались лабораторные крысы-самцы линии Wistar, интактные и с экспериментально вызванной гипергликемией. Было создано комплексное соединение PGPL и гепарина при соотношении компонентов 1: 1 (моль/моль), которое вводили один раз в сутки в течение 5 дней интраназально в дозе 1 мг/кг гипергликемическим крысам. Подобным образом вводили составные части комплекса в эквивалентных количествах. Определяли антикоагулянтную активность по тесту активированного частичного тромбопластинового времени, параметры фибринолиза по тестам суммарной, ферментативной и неферментативной фибринолитической активности, а также активности тканевого активатора плазминогена. Кроме того, проводилось измерение уровня глюкозы крови с использованием специальных тест-полосок. Результаты. Применение комплекса PGPL-гепарин у животных с гипергликемией приводило к нормализации уровня глюкозы крови, повышению антикоагулянтного и фибринолитического фона плазмы крови. Эти эффекты сохранялись в течение 6 дней после прекращения введения крысам пептидно-гепаринового комплекса. Заключение. Комплекс PGPL с гепарином проявляет комбинированное гипогликемическое, антикоагулянтное и фибринолитическое ферментативной и неферментативной природы действие при развитии экспериментальной гипергликемии. Исследуемый пептидно-гепариновый комплекс в перспективе может применяться для профилактики и лечения сахарного диабета 2 типа, осложняющегося повышенной свертываемостью крови. Ключевые слова: антикоагулянтная активность; уровень глюкозы крови; комплексное соединение; фибринолиз; глипролиновые пептиды; гипергликемия; сахарный диабет 2 типа

show abstract

“…One of the simplest glyprolins is the PGP peptide and studies have shown that the PGP peptide has antiulcer and anti‐inflammatory effects 63,64 . Based on this peptide, a number of synthetic derivatives have been obtained that are active against a wide range of pathological and stressful conditions 30,65‐68 …”

Section: Peptide Regulation In Ischemic Conditionsmentioning

confidence: 99%

Pharmacotranscriptomics of peptide drugs with neuroprotective properties

Dergunova

Filippenkov

Limborska

et al. 2020

Medicinal Research Reviews

View full text Add to dashboard Cite

Here we present a review of studies on the effects of peptides with neuroprotective properties on gene transcription in nerve cells. The few published works in this area clearly demonstrate massive changes in cell transcriptomes induced by peptides under normal conditions and under conditions of experimental brain ischemia. These changes significantly affect signaling and metabolic pathways, affecting various body systems and confirming the multiple target actions of peptides. The importance of noncoding RNAs in the regulation of these processes is shown, and we discuss the prospects of research for determining the main mechanisms of peptide regulation, which is necessary for the further development of drugs with targeted neuroprotective effects.

show abstract

Leucine-containing glyprolines (Pro-Gly-Pro-Leu and Leu-Pro-Gly-Pro): Participation in hemostatic reactions in vitro and in vivo in rats with blood coagulation and lipid metabolism disorders

Cited by 4 publications

References 4 publications

Insight into Glyproline Peptides’ Activity through the Modulation of the Inflammatory and Neurosignaling Genetic Response Following Cerebral Ischemia–Reperfusion

Insight into Glyproline Peptides’ Activity through the Modulation of the Inflammatory and Neurosignaling Genetic Response Following Cerebral Ischemia–Reperfusion

Pharmacology complex compound of pro-gly-pro-leu with heparin: hypoglycemic, fibrinolitic and anticoagulant effects in rats with hyperglycemia

Pharmacotranscriptomics of peptide drugs with neuroprotective properties

Contact Info

Product

Resources

About