Leucocyte and Platelet Groups and their
Practical Significance

Dausset, J.; Tangün, Y.

doi:10.1159/000465055

Cited by 1 publication

(1 citation statement)

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“…The platelet-specific antigens of PI Jl1 [26], PI A2 [25], DUZO [14], Koa [7], Kob [25], PIEl, PI E2 [22], Baka [4] and Leka [3] together with HLA-A, B, C antigens [17] and blood group ABO antigens [2,8] are alloantigens present on platelets, but little is known of other antigens [13]. Attempts were made to detect Lea on platelets using the mixed agglutination method, but they failed [2], André [ 1 ] and Dorf et al [10] suggested the presence of Lea antigen on platelets, because of the finding that hemagglutination became negative after absorption of anti-Lea erythrocyte antibodies with platelets.…”

Section: Introductionmentioning

confidence: 99%

Identification of Anti-Lea by Platelet Complement Fixation

Ando¹,

Itoh²

1986

Vox Sanguinis

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Two anti-Le^a sera which were able to detect Le^a antigen on platelets were identified in a screening for anti-platelet antibodies by means of a platelet complement fixation test. These two antisera hemolyzed erythrocytes without enzyme treatment. The anti-Le^a activity could be completely absorbed by red cells, platelets and lymphocytes of Le(a+b-) donors but not by cells from Le(a-b+) or Le(a-b-) donors. The antibody activity against red cells was eliminated by treatment of the antisera with dithiothreitol, thereby suggesting that the activity resided in the IgM class of immunoglobulins. As the anti-Le^a was more reactive at 37 °C than at room temperature against both red cells and platelets, we suggest that transfusion of platelets of Le^a-negative donors should be considered for patients with this type of anti-Le^a.

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