Leucocyte Depletion Improves Renal Function in Porcine Kidney Hemoreperfusion Through Reduction of Myeloperoxidase+ Cells, Caspase-3, IL-1β, and Tubular Apoptosis

Yang, Bin; Hosgood, Sarah A.; Harper, S.; Nicholson, Michael L.

doi:10.1016/j.jss.2010.07.044

Cited by 23 publications

(23 citation statements)

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“…Moreover, kidney I/R injury is known to engage cellular mediators of immunity, such as dendritic cells, neutrophils, macrophages, natural killer T, T, and B cells, which contribute to the pathogenesis of the renal injury [19]. Thus, leucocyte-depleted hemoreperfusion improved post-ischemic renal function and tubulointerstitial damage in a porcine model [20], and it has been shown that the immune response and, more specifically, lymphocytes (T and B) and dendritic cells participate as mediators of renal I/R injury [21].…”

Section: Introductionmentioning

confidence: 99%

Preconditioning with Triiodothyronine Improves the Clinical Signs and Acute Tubular Necrosis Induced by Ischemia/Reperfusion in Rats

et al. 2013

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BackgroundRenal ischemia/reperfusion (I/R) injury is manifested by acute renal failure (ARF) and acute tubular necrosis (ATN). The aim of this study was to evaluate the effectiveness of preconditioning with 3, 3, 5 triiodothyronine (T3) to prevent I/R renal injury.Methodology/Principal FindingsThe rats were divided into four groups: sham-operated, placebo-treated (SO-P), sham-operated T3- treated (SO- T3), I/R-injured placebo-treated (IR-P), and I/R-injured T3-treated (IR- T3) groups. At 24 h before ischemia, the animals received a single dose of T3 (100 μg/kg). Renal function and plasma, urinary, and tissue variables were studied at 4, 24, and 48 h of reperfusion, including biochemical, oxidative stress, and inflammation variables, PARP-1 immunohistochemical expression, and ATN morphology. In comparison to the SO groups, the IR-P groups had higher plasma urea and creatinine levels and greater proteinuria (at all reperfusion times) and also showed: increased oxidative stress-related plasma, urinary, and tissue variables; higher plasma levels of IL6 (proinflammatory cytokine); increased glomerular and tubular nuclear PARP-1 expression; and a greater degree of ATN. The IR-T3 group showed a marked reduction in all of these variables, especially at 48 h of reperfusion. No significant differences were observed between SO-P and SO-T3 groups.ConclusionsThis study demonstrates that preconditioning rats with a single dose of T3 improves the clinical signs and ATN of renal I/R injury. These beneficial effects are accompanied by reductions in oxidative stress, inflammation, and renal PARP-1 expression, indicating that this sequence of factors plays an important role in the ATN induced by I/R injury.

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Section: Introductionmentioning

confidence: 99%

Preconditioning with Triiodothyronine Improves the Clinical Signs and Acute Tubular Necrosis Induced by Ischemia/Reperfusion in Rats

et al. 2013

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“…In 1985, the Hopkins group of Baumgartner and associates investigated the method of leukocyte depletion for lung and/or heart preservation for transplantation [3,4]. In an article recently published in the Journal of Surgical Research, [5], the Leicester University transplant research group advance the knowledge of the effect of leukocyte depletion on various inflammatory cells and mediators in a kidney pig model of brief warm ischemia, cold preservation and hemoperfusion.…”

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confidence: 99%

“…Under this premise, Yang and associates [5] from the University of Leicester have elegantly studied an old phenomenon, but viewed it with current molecular science. They reviewed new mechanisms by which leukocyte depletion might exert its protective effect, namely, decreasing myeloperoxidaseþ cells by 91%, as well as diminishing tubular apoptosis and abolishing caspase-3 activity [5]. Furthermore, the expression of IL-1beta active subunit was partially diminished by leukocyte depletion and increased by hemoperfusion, which at the same time permitted increased of IL-1b precursor and HSP70.…”

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confidence: 99%

“…Yang's group [5] gives us some good elements to ponder pertaining to the protective role of leukocyte depletion. The studies considered in their work are currently more at the proof-of-concept state, and require further investigation into longer warm and cold ischemic times, particularly in the case of kidney transplantation.…”

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confidence: 99%

“…The Leicester group suggests that ''leukocyte depletion could be a potential approach for isolated kidney preservation for transplantation'' [5]. Even though this is a viable consideration, many steps would be essential prior to reaching this important conclusion.…”

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confidence: 99%

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Leukocyte Depletion, Ischemic Injury, and Organ Preservation

Toledo‐Pereyra¹

2011

Journal of Surgical Research

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Regional perfusion by extracorporeal membrane oxygenation of abdominal organs from donors after circulatory death: A systematic review

Shapey

Muiesan

2013

Liver Transpl

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Organs from donors after circulatory death (DCDs) are particularly susceptible to the effects of warm ischemia injury. Regional perfusion (RP) by extracorporeal membrane oxygenation (ECMO) is increasingly being advocated as a useful remedy to the effects of ischemia/reperfusion injury, and it has been reported to enable the transplantation of organs from donors previously deemed unsuitable. The MEDLINE, Embase, and Cochrane databases were searched, and articles published between 1997 and 2013 were obtained. A systematic review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Two hundred ten articles were identified, and 11 were eligible for inclusion. Four hundred eighty-two kidneys and 79 livers were transplanted from regional perfusion-supported donor after circulatory death (RP-DCD) sources. One-year graft survival was lower with uncontrolled RP-DCD liver transplantation, whereas 1-year patient survival was similar. Primary nonfunction and ischemic cholangiopathy were significantly more frequent with RP-DCDs versus donors after brain death (DBDs), but there was no difference in postoperative mortality between the 2 groups. The 1-year patient and graft survival rates for RP-DCD kidney transplantation were better than the rates with standard DCDs and were comparable to, if not better than, the rates with DBDs. At experienced centers, delayed graft function (DGF) for kidney transplantation from RP-DCDs was much less frequent in comparison with all other donor types. In conclusion, RP aids the recovery of DCD organs from ischemic injury and enables transplantation with acceptable survival. RP may help to increase the donor pool, but its benefits must still be balanced with the recognition of significantly higher rates of complications in liver transplantation. In kidney transplantation, significant reductions in DGF can be obtained Abbreviations: ABI, anoxic brain injury; ALS, advanced life support; ALT, alanine aminotransferase; AST, aspartate aminotrans-

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Leucocyte Depletion Improves Renal Function in Porcine Kidney Hemoreperfusion Through Reduction of Myeloperoxidase+ Cells, Caspase-3, IL-1β, and Tubular Apoptosis

Cited by 23 publications

References 32 publications

Preconditioning with Triiodothyronine Improves the Clinical Signs and Acute Tubular Necrosis Induced by Ischemia/Reperfusion in Rats

Preconditioning with Triiodothyronine Improves the Clinical Signs and Acute Tubular Necrosis Induced by Ischemia/Reperfusion in Rats

Leukocyte Depletion, Ischemic Injury, and Organ Preservation

Regional perfusion by extracorporeal membrane oxygenation of abdominal organs from donors after circulatory death: A systematic review

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