Leucocyte filtration of salvaged blood during cardiac surgery: effect on red blood cell function in concentrated blood compared with diluted blood

Gu, Yongquan; Vries, Adrianus J. de; Hagenaars, Johanna A. M.; Oeveren, Willem van

doi:10.1016/j.ejcts.2009.04.058

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…Another crucial threat is the hypotension triggered by the LDF-treated blood [ 15 , 16 ], which might be associated with the elevation of bradykinin [ 16 ] and interleukin-6 [ 17 ] during the transfusion of salvaged blood treated by LDF. In addition, LDF also markedly decreased the ATP content in erythrocytes [ 18 ]. Thus, Hansen’s group advocated gamma irradiation to eliminate tumor-laden blood and found that 50 Gy irradiation was sufficient to inactivate tumor cells in blood shed during cancer surgery with a reduction rate exceeding 10 logs [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

et al. 2015

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An understanding of how to safely apply intraoperative blood salvage (IBS) in cancer surgery has not yet been obtained. Here, we investigated the optimal dose of 137Cs gamma-ray irradiation for killing human hepatocarcinoma (HepG2), gastrocarcinoma (SGC7901), and colonic carcinoma (SW620) tumor cells while preserving co-cultured erythrocytes obtained from 14 healthy adult volunteers. HepG2, SGC7901, or SW620 cells were mixed into the aliquots of erythrocytes. After the mixed cells were treated with 137Cs gamma-ray irradiation (30, 50, and 100 Gy), tumor cells and erythrocytes were separated by density gradient centrifugation in Percoll with a density of 1.063 g/ml. The viability, clonogenicity, DNA synthesis, tumorigenicity, and apoptosis of the tumor cells were determined by MTT assay, plate colony formation, 5-ethynyl-2'-deoxyuridine (EdU) incorporation, subcutaneous xenograft implantation into immunocompromised mice, and annexin V/7-AAD staining, respectively. The ATP concentration, 2,3-DPG level, free Hb concentration, osmotic fragility, membrane phosphatidylserine externalization, blood gas variables, reactive oxygen species levels, and superoxide dismutase levels in erythrocytes were analyzed. We found that 137Cs gamma-ray irradiation at 50 Gy effectively inhibited the viability, proliferation, and tumorigenicity of HepG2, SGC7901, and SW620 cells without markedly damaging the oxygen-carrying ability or membrane integrity or increasing the oxidative stress of erythrocytes in vitro. These results demonstrated that 50 Gy irradiation in a standard 137Cs blood irradiator might be a safe and effective method of inactivating HepG2, SGC7901, and SW620 cells mixed with erythrocytes, which might help to safely allow IBS in cancer surgery.

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Section: Introductionmentioning

confidence: 99%

Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

et al. 2015

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“…Although all CS devices use the same theory of centrifugation and washing, this investigation clearly shows that the actual function of RBC products processed by different CSs varies significantly from one device to another. As mechanical processing affects the RBC function of salvaged blood (9,10), an important indicator of the performance of the CS is its capability to preserve RBC function as much as possible. The erythrocyte function test includes 2,3‐DPG as a surrogate for oxygen affinity, which is a major factor in tissue oxygen supply.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Effects of Three Cell Saver Devices on Erythrocyte Function During Cardiopulmonary Bypass Procedure—A Pilot Study

Wang

Zhang

et al. 2012

Artificial Organs

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Cell salvage devices are routinely used to process red blood cells (RBCs) shed during cardiac surgery. The purpose of this study was to evaluate three commercially available cell saver (CS) devices in terms of erythrocyte function and the quality of washed RBCs during cardiopulmonary bypass (CPB). Thirty patients undergoing CPB were randomly allocated to three CS devices: Group C (Cell Saver 5+; Haemonetics, n = 10), Group M (autolog; Medtronic, n = 10), and Group F (CATS; Fresenius HemoCare, n = 10). Blood samples were collected from reservoirs and transfusion bags. Reservoirs and washed RBCs were analyzed for erythrocyte aggregation index, deformation index (DI) and hematocrit viscosity, 2,3‐diphosphoglycerate (2,3‐DPG), hematocrit (Hct), hemoglobin (Hb), free Hb removal (ΔfHb), glucose (Glu), lactate (Lac), and blood urea nitrogen. After processing, Groups C (P = 0.026) and M (P = 0.032) had relatively higher erythrocyte DI compared with Group F. Group C had lower Δ2,3‐DPG compared with Groups M (P = 0.001) and F (P = 0.001). Group F provided the maximum concentration of Hct (P = 0.021; 0.046) and Hb (P = 0.008; 0.013). In addition, Groups C (P = 0.035) and M (P = 0.038) had a higher removal of fHb (ΔfHb), differing significantly with Group F. In conclusion, CS devices use the same theory of centrifugation; however, based on different designs, the function of the washed erythrocyte and undesirable content removal efficiency differs widely from one device to another.

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Cell salvage for minimising perioperative allogeneic blood transfusion in adults undergoing elective surgery

Lloyd,

Geneen,

Bernhardt

et al. 2023

Cochrane Database of Systematic Reviews

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Leucocyte filtration of salvaged blood during cardiac surgery: effect on red blood cell function in concentrated blood compared with diluted blood

Abstract: Leucocyte filtration of the diluted salvaged blood during cardiac surgery does not affect RBC function, but it tends to deplete the ATP content of RBCs as the salvaged blood has been concentrated prior to filtration.

Cited by 5 publications

References 23 publications

Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

Comparison of the Effects of Three Cell Saver Devices on Erythrocyte Function During Cardiopulmonary Bypass Procedure—A Pilot Study

Cell salvage for minimising perioperative allogeneic blood transfusion in adults undergoing elective surgery

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