Leucocyte metabolism in human saliva

Eichel, Bertram; Lisanti, Vincent F.

doi:10.1016/0003-9969(64)90062-7

Cited by 27 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The significance of these observations and the potential relative efficacy of toothpastes within the oral cavity in terms of the metabolic parameters under consideration here remain indeterminate, since these experiments like most prior studies of salivary and plaque metabolism, for example pH measurements, have been obtained using human whole salivas which are characterized by the absence of viable healthy leucocytes normally present in the oral cavity, Eichel and Lisanti (1964).…”

Section: Discussionmentioning

confidence: 99%

“…Paraffin stimulated human whole salivas were collected in tubes stored in a cracked ice bath. Sediment fractions were prepared either at 50 xg for 3 minutes, Eichel and Lisanti (1964)…”

Section: Methods Saliva Collections and Samplingmentioning

confidence: 99%

“…

Most investigators, concerned with the external environment of the teeth and how this environment may contribute to the development and progress of the caries lesion, have dealt with various metabolic parameters involving saliva and plaque.The biological material of choice most frequently employed in such studies has been paraffin stimulated human whole saliva or sediment preparations made therefrom, the latter sometimes being identified with plaque, debris (including food), and most often bacteria. Beginning with the pioneering work of Magitot (1878) in this field, and regardless of the method of analysis employed, virtually all investigators have attributed detectable metabolic activities found in human whole saliva, saliva sediment, and plaque to oral micro-organisms.Recently we, Eichel (1961), Eichel and Lisanti (1964), seriously challenged this contention and found that paraffin stimulated human whole salivas essentially exhibited the unregulated metabolism of protoplasmic fragments derived from leucocytes within the oral cavity, while the oral bacteria contribution to the salivas total metabolic pool was of a low order of magnitude. Because of the large number of epithelial cells present in such saliva collections, we investigated and demonstrated in related studies (unpublished experiments) that preparations of sloughable epithelial cells from the oral mucosa also yielded low metabolic activity.

…”

mentioning

confidence: 99%

“…Recently we, Eichel (1961), Eichel and Lisanti (1964), seriously challenged this contention and found that paraffin stimulated human whole salivas essentially exhibited the unregulated metabolism of protoplasmic fragments derived from leucocytes within the oral cavity, while the oral bacteria contribution to the salivas total metabolic pool was of a low order of magnitude. Because of the large number of epithelial cells present in such saliva collections, we investigated and demonstrated in related studies (unpublished experiments) that preparations of sloughable epithelial cells from the oral mucosa also yielded low metabolic activity.…”

mentioning

confidence: 99%

See 3 more Smart Citations

The Effects of Dentifrices on Human Saliva Metabolism

Lisanti

Eichel

Szabo

1965

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

“…Paraffin stimulated human whole salivas were collected in tubes stored in a cracked ice bath. Sediment fractions were prepared either at 50 xg for 3 minutes, Eichel and Lisanti (1964)…”

Section: Methods Saliva Collections and Samplingmentioning

confidence: 99%

“…

…”

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

The Effects of Dentifrices on Human Saliva Metabolism

Lisanti

Eichel

Szabo

1965

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

“…Glycosidases and proteases potentially capable of degrading salivary components are elabo-rated by a variety of oral bacteria (4,7,18,19). In addition, lysosomal enzymes derived from leukocytes in the gingival crevice may also be present in oral fluids (5,23). Such bacterial or host-derived enzymes could alter receptors for bacteria in the pellicle and thereby affect bacterial attachment and colonization on the teeth.…”

mentioning

confidence: 99%

Enzymatic Modification of Bacterial Receptors on Saliva-Treated Hydroxyapatite Surfaces

Gibbons

Etherden

1982

Infect Immun

View full text Add to dashboard Cite

Certain properties of experimental pellicles formed by the adsorption of salivary components on hydroxyapatite surfaces change over time. To determine whether enzymes likely to be present in the oral environment could induce such changes, pellicles were treated with saliva which had been incubated for 18 h at 35°C to promote the elaboration of microbial enzymes. This treatment markedly reduced the numbers of Streptococcus mutans MT3 and JBP and S. sanguis FC-1 and C5 cells which attached, but it had little or no effect on the attachment of S. mitis RE7, Actinomyces viscosus LY7 and CK-8, Bacteroides gingivalis 381, or B. melaninogenicus subsp. intermedius 581. Heating the incubated saliva at 60°C for 30 min partially reduced its pellicle-modifying activity, whereas heating at 80°C for 30 min or 100°C for 15 min completely eliminated such activity. This indicated that the saliva contained heat-labile substances, presumably enzymes, which could affect the pellicle receptors involved in the attachment of S. mutans and S. sanguis. Treatment of saliva-treated hydroxyapatite with commerically obtained enzyme preparations also affected bacterial attachment. Thus, treatment with galactose oxidase reduced the numbers of the S. mutans strains which attached, whereas treatment with neuraminidase reduced the adsorption of S. sanguis FC-1 but not that of S. sanguis C5. Treatment with ,B-glucosidase preparations derived from almonds significantly reduced the attachment of all of the streptococcal strains studied, but, when subjected to isoelectric fractionation, the adherence-inhibiting activity did not correlate directly with P-glucosidase activity. Treatment of the pellicles with trypsin or eight other glycosidases did not affect streptococcal attachment. Exposure of the enzymatically modified pellicles to fresh saliva did not restore the streptococcal receptors. Collectively, the data suggest that some bacterial receptors in the pellicle coating of teeth can be modified by enzymes likely to be present in the oral environment, and these interactions may affect oral bacterial ecology.

show abstract

The effect of saliva on the metabolism of the oral flora

Hay

Hartles

1965

Archives of Oral Biology

View full text Add to dashboard Cite

Leucocyte metabolism in human saliva

Cited by 27 publications

References 7 publications

The Effects of Dentifrices on Human Saliva Metabolism

The Effects of Dentifrices on Human Saliva Metabolism

Enzymatic Modification of Bacterial Receptors on Saliva-Treated Hydroxyapatite Surfaces

The effect of saliva on the metabolism of the oral flora

Contact Info

Product

Resources

About