Leucocyte Typing

Ling, N. R.; Johnson, George; MacLennan, I. C. M.; Hansel, T.T.

doi:10.1016/s0140-6736(88)91108-7

Cited by 3 publications

(1 citation statement)

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“…Although the relative molecular mass of CD40 molecule was reported to be 50 kDa, in our hands 1251-or internally labeled CD40 was found to be much smaller (approximately 45 kDa). Recent biochemical analysis also showed that the relative molecular mass of CD40 was 43 or 44 kDa [34]. [21] is designated as + 1.…”

Section: The Intracytoplasmic Region Essential For Signalmentioning

confidence: 99%

Identification of the intracytoplasmic region essential for signal transduction through a B cell activation molecule, CD40

et al. 1990

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CD40 is a 45-kDa glycoprotein expressed on human B lineage cells. Anti-CD40 induces the proliferation of B cells and the extracellular region of CD40 is related to those of a certain kind of growth factor receptors. Therefore, it has been proposed that CD40 might be a receptor for a molecule involved in the growth regulation of B cells. The cDNA coding for CD40 was transfected into the murine B lymphoma cell line M12 and the murine thymoma cell line EL4. The growth of both M12 and EL4 transfectants expressing human CD40 was inhibited by anti-CD40. Phorbol 12-myristate 13-acetate (PMA) augmented the growth inhibitory effects of anti-CD40 on transfectants. The CD40 molecule was constitutively phosphorylated not only in human tonsil B cells but also in transfectants expressing CD40. PMA augmented the phosphorylation of CD40 in these cells. These results indicate that in spite of the growth inhibitory effect of anti-CD40, the augmentative effect of PMA is conserved in CD40+ transfectants and suggest that the transfectant might be useful for the study of signal transduction mechanism through CD40. To investigate which part of the CD40 molecule is important for signal transduction, transfectants expressing mutant CD40 cDNA were established and their growth response to anti-CD40 was evaluated. The mutant molecule, which had an Ala for Thr substitution at position 234, and the deletion mutants lacking Thr234 were inactive in growth signal transduction, indicating that Thr234 itself or the region around Thr234 is essential for signal transduction through CD40.

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Section: The Intracytoplasmic Region Essential For Signalmentioning

confidence: 99%

Identification of the intracytoplasmic region essential for signal transduction through a B cell activation molecule, CD40

et al. 1990

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Colorectal Carcinoma: Key Issues

Greig¹,

Poste²

1990

Colon Cancer Cells

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Tests of adrenal insufficiency

Agwu¹,

Spoudeas²,

Hindmarsh³

et al. 1999

Archives of Disease in Childhood

100

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Aim-In suspected adrenal insuYciency, the ideal test for assessing the hypothalamo-pituitary-adrenal axis is controversial. Therefore, three tests were compared in patients presenting with symptoms suggestive of adrenal insuYciency. Method-Responses to the standard short Synacthen test (SSST), the low dose Synacthen test (LDST), and the 08:00 hour serum cortisol concentration were measured in 32 patients. A normal response to the synacthen test was defined as a peak serum cortisol of > 500 nmol/l and/or incremental concentration of > 200 nmol/l. The sensitivity and specificity of the 08:00 hour serum cortisol concentration compared with other tests was calculated. Results-Three patients had neither an adequate peak nor increment after the SSST and LDST. All had a serum 08:00 hour cortisol concentration of < 200 nmol/l. Eight patients had abnormal responses by both criteria to the LDST but had normal responses to the SSST. Three reported amelioration of their symptoms on hydrocortisone replacement. Twenty one patients had a normal response to both tests (of these, 14 achieved adequate peak and increment after both tests and seven did not have an adequate peak after the LDST but had a normal increment). The lowest 08:00 hour serum cortisol concentration above which patients achieved normal responses to both the LDST and SSST was 500 nmol/l. At this cut oV value (compared with the LDST), the serum 08:00 hour cortisol concentration had a sensitivity of 100% but specificity was only 33%. Conclusion-The LDST revealed mild degrees of adrenal insuYciency not detected by the SSST. The value of a single 08:00 hour serum cortisol concentration is limited. (Arch Dis Child 1999;80:330-333)

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Leucocyte Typing

Cited by 3 publications

References 0 publications

Identification of the intracytoplasmic region essential for signal transduction through a B cell activation molecule, CD40

Identification of the intracytoplasmic region essential for signal transduction through a B cell activation molecule, CD40

Colorectal Carcinoma: Key Issues

Tests of adrenal insufficiency

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