Leukapheresis after high‐dose chemotherapy and autologous peripheral blood progenitor cell transplantation: a novel approach to harvest a second autograft

Abstract

Collection of PBPCs is possible in most patients during the recovery phase of hematopoiesis after HDCT plus autografting, and the number of circulating PBPCs may be related to the CD34+ cell dose transfused by the preceding autograft.

Comparison of two apheresis systems for the collection of CD14+ cells intended to be used in dendritic cell culture

“…The MNC programs of Astec and Cobe Spectra are semiautomated and both need control by an operator. However, there are fundamental differences of either collection procedure 19 . Applying Astec, the whole buffy coat is collected from the beginning of the procedure (discontinuous collection).…”

“…However, there are fundamental differences of either collection procedure. 19 Applying Astec, the whole buffy coat is collected from the beginning of the procedure (discontinuous collection). Using Spectra (continuous collection) the collecting process of MNCs starts not until the buffy-coat layer has stratified within a duration of 10 to 20 minutes that represents about 10 to 20 percent of the whole collection time in 5-L procedures ( Table 3).…”

Long‐term cryopreservation of autologous stem cell grafts: a clinical and experimental study of hematopoietic and immunocompetent cells

“…It may be challenging to harvest sufficient stem cells at the time of relapse when the disease is at a more advanced stage and the patients have received chemotherapy that has reduced the stem cell pool. 4 For this reason some centers prefer to harvest and cryopreserve stem cells for two ASCTs before the first transplantation. 5 The second dose of stem cells may require storage for several years before it is utilized, but the effect of such long-term storage of autologous stem cell grafts has not been characterized in detail previously.…”

Evaluation of the COM.TEC cell separator in predicting the yield of harvested CD34+ cells

“…5,6 Currently, dose-escalated chemotherapy regimens, sequential courses of high-dose chemotherapy, and PBPC collection in healthy donors increase the needs for leukapheresis and the quantity of collected PBPCs. [7][8][9][10] Automation of PBPC harvesting may help to cope with these requirements and to perform leukapheresis in a convenient, safe, and efficient manner. 11,12 The success of hematopoietic transplantation mainly depends on the transfusion of a sufficient CD34+ cell dose to reconstitute patients' hematopoiesis rapidly.…”

“…The combination of cytotoxic chemotherapy plus growth factors is an effective way to mobilize autologous CD34+ cells from the marrow into PB 5,6 . Currently, dose‐escalated chemotherapy regimens, sequential courses of high‐dose chemotherapy, and PBPC collection in healthy donors increase the needs for leukapheresis and the quantity of collected PBPCs 7‐10 . Automation of PBPC harvesting may help to cope with these requirements and to perform leukapheresis in a convenient, safe, and efficient manner 11,12 .…”