Leukemia blast-induced T-cell anergy demonstrated by leukemia-derived dendritic cells in acute myelogenous leukemia

CD8 T-cell responses to Wilms tumor gene product WT1 and proteinase 3 in patients with acute myeloid leukemia

“…One possible explanation for this lower amount of IFN-␥ produced per cell might be a lower functional avidity of T cells, as suggested by a recent study. 30 Immune responses in leukemia patients may be hampered by leukemia-specific immunosuppression and general immunosuppression, attributed to T-cell dysfunction induced by leukemia blasts 31 and by cytotoxic therapy. 32 Patients with advanced tumors often show a diminished T-cell response.…”

The effects of STI571 on antigen presentation of dendritic cells generated from patients with chronic myelogenous leukemia

“…11 Briefly, CD1a þ dendritic cells, which were separated on a microbeads column, were placed on the slide glass and denatured by incubation in 70% formamide/2 Â SSC at 71 C for 2 min. The denatured cells were hybridized with a mixture of SpectrumOrange-labelled DNA probes specific for the 9q34 chromosome breakpoint (abl) (VYSIS, Downers Grove, IL) and SpectrumGreen-labelled DNA probes specific for the 22q11 chromosome breakpoint (bcr) (VYSIS).…”

Induction of recipient cell-specific donor T-cell anergy by UV-C-irradiated recipient immature monocyte-derived dendritic cells

“…Escape of leukemia cells from anti-leukemia immunity was suggested to be associated with T-cell anergy caused by leukemia blasts. 23 By using a similar experimental design with the first and the second MLC, we aimed to demonstrate the generation of T-cell anergy by using UV-C-irradiated human mo-DCs. The present study demonstrated that the irradiation of mo-DCs with UV-C induced the reduction of the expression of DC-associated surface phenotypes such as CD83, CD80, CD86, HLA-DR, CD40 and CD58 and the decrease of antigen presenting ability by UV-C-irradiated mo-DCs.…”