Leukocyte Adhesion Molecules as Biocompatibility Markers for Hemodialysis Membranes

Appen, K. von; Goolsby, Charles L.; Mehl, Patrick M.; Goewert, Robert R.; Ivanovich, Peter

doi:10.1097/00002480-199407000-00071

Cited by 16 publications

(5 citation statements)

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“…22,23 Leukocytes show an altered expression of various surface marker proteins on contact with noncompatible materials, 24 and the quantification of these markers by flow cytometry has been used as a tool to assess material incompatibility. 25 Comparable and unaltered expression profiles (p > 0.05) for CD11b/CD18, 26 CD45, 27 and CD14 27 by macrophages cultured on the hydrogel and TCPS control (Fig. 3) suggest the hydrogel's nonactivated nature toward macrophages.…”

Section: Discussionmentioning

confidence: 96%

Biocompatible hydrogel supports the growth of respiratory epithelial cells: Possibilities in tracheal tissue engineering

Risbud

Endres

Ringe

et al. 2001

J. Biomed. Mater. Res.

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Extensive tracheal defect reconstruction is a major challenge in plastic and reconstructive surgery. The lack of an epithelial lining on the luminal surfaces of tracheal prostheses is among the major causes of their failure. Chitosan-gelatin hydrogels were synthesized for the development of biocompatible, growth-supportive substrata for respiratory epithelial cells. We employed J774 macrophages to test the immunocompatibility of this gel. The hydrogel did not exert a cytotoxic effect on macrophages, as confirmed by tetrazolium reduction and neutral red uptake assay. Flow cytometric analysis of macrophages cultured on the hydrogel showed a comparable expression of activation markers CD11b/CD18, CD45, and CD14 to the control. Semiquantitative RT-PCR results showed an absence of upregulation of interleukin-6 (IL-6) and TNF-alpha in these macrophages with respect to the controls. Primary human respiratory epithelial cells cultured on the hydrogel showed proper attachment, normal morphology, and growth. A small proportion of cells on the hydrogel showed synchronously beating cilia. RT-PCR analysis showed that cells on the hydrogel expressed mucins 2 and 5 and cytokeratin 13, which are markers for secretory goblet and squamous cells, respectively. All these results demonstrate that the hydrogel supports the growth of a mixed population of differentiated epithelial cells. This hydrogel is suitable as a culture substratum for respiratory epithelial cells and could be used as a potential candidate for coating tracheal prostheses.

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Section: Discussionmentioning

confidence: 96%

Biocompatible hydrogel supports the growth of respiratory epithelial cells: Possibilities in tracheal tissue engineering

Risbud

Endres

Ringe

et al. 2001

J. Biomed. Mater. Res.

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“…CD11c/CD18 (CR4, p150/95), another member of the leukocyte integrin family, also showed an upregulation during dialysis with unmodified cellulose membranes (13,15,16); however this phenomenon was not confirmed by Stuard et al (10). The expression of CD11a/CD18 (LFA-1, leukocyte function antigen-1) with whatever membrane did not change during dialysis, (8, 10,11,13,15).…”

Section: Patho-physiologic Mechanismsmentioning

confidence: 95%

“…The expression of CD11a/CD18 (LFA-1, leukocyte function antigen-1) with whatever membrane did not change during dialysis, (8, 10,11,13,15).…”

Section: Patho-physiologic Mechanismsmentioning

confidence: 97%

Hemodialysis-Related Bioincompatibility and Adhesion Molecules

1998

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“…Appen et a! measured the effects of hemodialysis membrane materials on leukocyte activation (34). Following exposure of blood to the membrane surfaces, neutrophil CD II b expression was increased within the first three minutes, but returned to pre-exposure levels after 60 minutes.…”

Section: Perflubron Mean Channel Of Fluorescencementioning

confidence: 99%

Perflubron Emulsion Does Not Cause Neutrophil (PMN) Activation In- Vivo.

Wilson,

Hokama,

Lai

et al. 1997

J Extra Corpor Technol

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Earlier, we reported that in-vitro incubation of blood for ten minutes with the perfluorocarbon (PFC) emulsion Fluosol increased leukocyte activation as determined by adhesion to nylon fiber. In this study, we examined if in-vivo treatment with these PFC emulsions affected the expression of the leukocyte adhesion protein CD11b (primarily found on PMNs) and the generation of leukocyte-derived reactive oxygen species (ROS, oxygen free radicals). Rats were anesthetized and catheterized. Three groups were studied: 1) a phosphate buffered saline (PBS) control group (n=6), 2) a group treated with Fluosol emulsion (1.08g PFC/kg, n=6) and 3) a group treated with perflubron emulsion (1.08g PFC/kg, n=6). Blood samples were taken before and 10, 20, 40 and 60 minutes after treatment for hematology and analysis of PMN CD11b expression and ROS production using flow cytometry. We found that Fluosol caused significant increases in both neutrophil surface expression of CD11b and ROS generation (p<0.05, ANOVA). In the Fluosol group, the peak responses in PMN CD11b expression and ROS production were observed ten minutes after treatment. In contrast, treatment with perflubron emulsion did not cause a significant increase in CD11b expression nor an increase in ROS production at any time after treatment. These findings suggest that Fluosol causes a transient PMN activation in-vivo. The activation of circulating PMNs, in-vivo, is sufficient to significantly enhance oxygen derived free radical production. The lack of a PMN response to perflubron emulsion in-vivo suggests that this agent is not likely to induce a leukocyte-mediated inflammatory response.

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Leukocyte Adhesion Molecules as Biocompatibility Markers for Hemodialysis Membranes

Cited by 16 publications

References 0 publications

Biocompatible hydrogel supports the growth of respiratory epithelial cells: Possibilities in tracheal tissue engineering

Biocompatible hydrogel supports the growth of respiratory epithelial cells: Possibilities in tracheal tissue engineering

Hemodialysis-Related Bioincompatibility and Adhesion Molecules

Perflubron Emulsion Does Not Cause Neutrophil (PMN) Activation In- Vivo.

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