Leukocyte Antigens and the Mixed Lymphocyte
Culture Reaction

Iványi, D.; RychlÍková, M.; Sasportes, M.; Iványi, P.; Dausset, D.

doi:10.1159/000464594

Cited by 2 publications

(2 citation statements)

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“…Since then the mixed leucocyte culture (MLC) has been used extensively as a tool to assay cellular immune reactions. Experiments in rats [11,31,36], mice [1,10] and man [2,4,6,15,33] have been generally accepted as proof that major differences in histocompatibility antigens are the crucial factor giving rise to the lymphocyte transformation in MLC. Reports that solubilized histocompatibility antigens could also induce lymphocyte transformation further strengthened this view [37].…”

mentioning

confidence: 99%

Inability of UV-Irradiated Lymphocytes to Stimulate Allogeneic Cells in Mixed Lymphocyte Culture

Lindahl-Kiessling¹,

Säfwenberg²

1971

Int Arch Allergy Immunol

115

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confidence: 99%

Inability of UV-Irradiated Lymphocytes to Stimulate Allogeneic Cells in Mixed Lymphocyte Culture

Lindahl-Kiessling¹,

Säfwenberg²

1971

Int Arch Allergy Immunol

115

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“…Studies by other workers have failed to detect this type of reaction using uptake of H3 Thymidine into DNA [2] or the degree of blast cell transformation as criteria of transformation [12]. More recently, however, Ceppelini et al [4], using DNA synthesis as the criteria for transformation and Ivanyi et al, using morphological assessment [13] have confirmed the existence of such a negative class of cultures.…”

Section: Discussionmentioning

confidence: 98%

The Mixed Leucocyte Reaction: Studies Using Leucocytes From Unrelated Human Subjects

Elves¹

1969

Vox Sanguinis

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The MLR has been studied in 87 culture combinations assessed by morphological techniques. The upper limit of negativity in these cultures was taken as the mean index of transformation found in all control cultures plus 2 standard deviations. Two sampling times (4 days and 6 days) were used. 6/87 cultures were within the negative range on both occasions. Of the positive cultures, 4 different patterns of response were observed and are described. The significance of these patterns is not yet clear. Similar results were found in those 69 cultures which were assessed by the degree of incorporation of I125 iododeoxyuridine. This method is more sensitive than the morphological technique and fewer cultures could be regarded as within the negative range (mean of controls ± 2 standard deviations). The same patterns of response were observed. The result of the MLR is not very reproducible when assessed by height of response. Non-technical factors affecting reproducibility are discussed. It was found that a period of cooling of cells prior to culture increased the index of transformation.

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Leukocyte Antigens and the Mixed Lymphocyte Culture Reaction

Cited by 2 publications

References 10 publications

Inability of UV-Irradiated Lymphocytes to Stimulate Allogeneic Cells in Mixed Lymphocyte Culture

Inability of UV-Irradiated Lymphocytes to Stimulate Allogeneic Cells in Mixed Lymphocyte Culture

The Mixed Leucocyte Reaction: Studies Using Leucocytes From Unrelated Human Subjects

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