Leukocyte Beta2-Integrins; Genes and Disease

Morrison, MacPherson M

doi:10.4172/2157-7412.1000154

Cited by 5 publications

(2 citation statements)

References 56 publications

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“…26 MAC-1 (CD11b/CD18) can promote phagocytosis of complement opsonized bodies through binding to iC3b. 27 Excessive stimulation of neutrophils can cause neutrophil exhaustion. 28 Platelet-neutrophil interactions have also been shown to enhance neutrophil phagocytic capabilities.…”

Section: Discussionmentioning

confidence: 99%

Neutrophil Structural and Functional Alterations After High Mechanical Shear Stress Exposure

et al. 2023

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Patients on mechanical circulatory support are prone to infections, increasing morbidity and mortality. These circulatory support devices generate high mechanical shear stress (HMSS) that can causes trauma to blood. When leukocytes become damaged, their immune response function may be impaired or weakened, leading to increased infection vulnerability. This study examined neutrophil structural and functional alterations after exposure to 75, 125, and 175 Pa HMSS for 1 second. Human blood was exposed to three levels of HMSS using a blood shearing device. Neutrophil morphological alteration was characterized by examining blood smears. Flow cytometry assays were used to analyze expression levels of CD62L and CD162 receptors, activation level (CD11b), and aggregation (platelet-neutrophil aggregates). Neutrophil phagocytosis and rolling were examined via functional assays. The results show neutrophil structure (morphology and surface receptors) and function (activation, aggregation, phagocytosis, rolling) were significantly altered after HMSS exposure. These alterations include cell membrane damage, loss of surface receptors (CD62L and CD162), initiation of activation and aggregation, upregulation of phagocytic ability and increased rolling speed. The alterations were the most severe after 175 Pa exposure. HMSS caused damage and activation of neutrophils, potentially impairing normal neutrophil function, leading to weakened immune defense and increasing a patient's vulnerability to infections.

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Section: Discussionmentioning

confidence: 99%

Neutrophil Structural and Functional Alterations After High Mechanical Shear Stress Exposure

et al. 2023

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“…One of the most important group of adhesion molecules on leukocytes are the β2-integrins. β2-integrins are transmembrane proteins consisting of the β-chain CD18 which forms heterodimers with the four α-chains: CD11a, CD11b, CD11c, and CD11d, forming 4 different receptors e.g., CD11a/CD18 (LFA-1, α L β 2 , ITAL Ag), CD11b/CD18 (Mac-1, α M β 2 , CR3, ITAM Ag), CD11c/CD18 (p150p95, α X β 2 , CR4, ITAX Ag), CD11d/CD18 (α D β 2 , ITAD Ag) (1).…”

Section: Introductionmentioning

confidence: 99%

A β2-Integrin/MRTF-A/SRF Pathway Regulates Dendritic Cell Gene Expression, Adhesion, and Traction Force Generation

et al. 2019

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β2-integrins are essential for immune system function because they mediate immune cell adhesion and signaling. Consequently, a loss of β 2 -integrin expression or function causes the immunodeficiency disorders, Leukocyte Adhesion Deficiency (LAD) type I and III. LAD-III is caused by mutations in an important integrin regulator, kindlin-3, but exactly how kindlin-3 regulates leukocyte adhesion has remained incompletely understood. Here we demonstrate that mutation of the kindlin-3 binding site in the β2-integrin (TTT/AAA-β2-integrin knock-in mouse/KI) abolishes activation of the actin-regulated myocardin related transcription factor A/serum response factor (MRTF-A/SRF) signaling pathway in dendritic cells and MRTF-A/SRF-dependent gene expression. We show that Ras homolog gene family, member A (RhoA) activation and filamentous-actin (F-actin) polymerization is abolished in murine TTT/AAA-β2-integrin KI dendritic cells, which leads to a failure of MRTF-A to localize to the cell nucleus to coactivate genes together with SRF. In addition, we show that dendritic cell gene expression, adhesion and integrin-mediated traction forces on ligand coated surfaces is dependent on the MRTF-A/SRF signaling pathway. The participation of β2-integrin and kindlin-3-mediated cell adhesion in the regulation of the ubiquitous MRTF-A/SRF signaling pathway in immune cells may help explain the role of β2-integrin and kindlin-3 in integrin-mediated gene regulation and immune system function.

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In silico transcriptomic mapping of integrins and immune activation in Basal-like and HER2+ breast cancer

et al. 2021

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Leukocyte Beta2-Integrins; Genes and Disease

Cited by 5 publications

References 56 publications

Neutrophil Structural and Functional Alterations After High Mechanical Shear Stress Exposure

Neutrophil Structural and Functional Alterations After High Mechanical Shear Stress Exposure

A β2-Integrin/MRTF-A/SRF Pathway Regulates Dendritic Cell Gene Expression, Adhesion, and Traction Force Generation

In silico transcriptomic mapping of integrins and immune activation in Basal-like and HER2+ breast cancer

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