Leukocyte Count and Differential Leukocyte Count of Carp (Cyprinus carpio Linn) after Infected by Aeromonas salmonicida

Afiyanti, Abdika Dwi; Yuliani, M. Gandul Atik; Handijatno, Didik

doi:10.5220/0007546705450549

Cited by 4 publications

(3 citation statements)

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“…The delayed peak (at 14 days) in the bath challenge suggested that adaptive immune response took longer to develop in the bath route. An increase in leukocytes after bacterial infections was observed in other fish species [30,[58][59][60], which is consistent with our finding after infection with M. viscosa (Figure 2).…”

Section: Discussionsupporting

confidence: 92%

Haemato-Immunological Response of Immunized Atlantic Salmon (Salmo salar) to Moritella viscosa Challenge and Antigens

Ghasemieshkaftaki,

Cao,

Hossain

et al. 2024

Vaccines

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Winter ulcer disease is a health issue in the Atlantic salmonid aquaculture industry, mainly caused by Moritella viscosa. Although vaccination is one of the effective ways to prevent bacterial outbreaks in the salmon farming industry, ulcer disease related to bacterial infections is being reported on Canada’s Atlantic coast. Here, we studied the immune response of farmed immunized Atlantic salmon to bath and intraperitoneal (ip) M. viscosa challenges and evaluated the immunogenicity of M. viscosa cell components. IgM titers were determined after infection, post boost immunization, and post challenge with M. viscosa. IgM+ (B cell) in the spleen and blood cell populations were also identified and quantified by 3,3 dihexyloxacarbocyanine (DiOC6) and IgM-Texas red using confocal microscopy and flow cytometry. At 14 days post challenge, IgM was detected in the serum and spleen. There was a significant increase in circulating neutrophils 3 days after ip and bath challenges in the M. viscosa outer membrane vesicles (OMVs) boosted group compared to non-boosted. Lymphocytes increased in the blood at 7 and 14 days after the ip and bath challenges, respectively, in OMVs boosted group. Furthermore, a rise in IgM titers was detected in the OMVs boosted group. We determined that a commercial vaccine is effective against M. viscosa strain, and OMVs are the most immunogenic component of M. viscosa cells.

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Section: Discussionsupporting

confidence: 92%

Haemato-Immunological Response of Immunized Atlantic Salmon (Salmo salar) to Moritella viscosa Challenge and Antigens

Ghasemieshkaftaki,

Cao,

Hossain

et al. 2024

Vaccines

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“…Regarding to positive control, similar results of leucocytosis were recorded after bacterial infection in Tilapia in order to tackle the infection (Mazrouh et al, 2015) which, may be attributed to lymphocytosis and neutrophilia. As well Afiyanti et al (2018) estimated the leukocytes and D.L.C in carp 3 days after A. salmonicida infection and found a significant leukocytosis, eosinophilia, neutrophilia, lymphocytosis and monocytosis and non-significant basophilic count. In treated groups the extract was capable of enhancing the act of the immune system by generating more leucocytes, as a result giving the fish more ability to restrain the bacterial growth in the fish body.…”

Section: Discussionmentioning

confidence: 89%

Trying to Use Antibiotics Alternatives to Raise Immune Efficiency and Growth Performance in Tilapia Nilotica

Khoris

El-Sherbeny

2022

Assiut Veterinary Medical Journal

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This study examined in vitro, the antibacterial activity of Malva parviflora leaves ethanolic extract against Pseudomonas fluorescens and its minimum inhibitory and minimum bactericidal-concentrations (MIC, MBC), identified its biological compounds responsible f or its efficacy by Gas Chromatography-Mass Spectrometer (GC-MS) device besides evaluated its effect in vivo on Nile tilapia fish as a medicinal plant to combat P. fluorescens challenge. Two hundred twenty-five fishes were divided into five groups; G1: negative control. G2: positive control; infected at the 60 days (1x103CFU/0.2ml I/M). G3, G4 and G5 were fed with (0.25, 0.5 and 1 g/kg) respectively (60 days), then infected. On the thirty, sixty days and post challenge, blood samples were collected. Sensitivity test revealed the susceptibility of P. fluorescens to the extract; MIC and MBC were 3.90 and 31.25 mg/ml respectively. Body weight, phagocyte nitric oxide and lysozyme activity increased significantly in all treated groups than controls especially G4 while both G3 and G4 increased the survival rate to 80% compared to G2 (40%). Total leucocytic, lymphocyte (L) and monocyte (M) counts increased significantly in all treated groups than G1 throughout the study. Aspartate (AST) and alanine aminotransferases (ALT), alkaline phosphatase (ALP), urea and creatinine showed no significant difference between G3 and G1, while G4 and G5 increased in a dose -dependent manner. Total protein (TP), albumin and globulin revealed no significant difference among groups. Malondialdehyde (MDA) showed the best decrease in G3 than G1. Post challenge all treated groups elicited a significant decrease than G2. Superoxide dismutase (SOD) and reduced glutathione (GSH) were significantly higher in all treated groups than the controls in varying degrees. Finally, the used extract enhanced the fish performance, immune system and antioxidant biomarkers without any side effects on liver and kidney funct ions at 0.25 g/kg, while 0.5 g/kg gave the highest weight gain.

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“…It has been reported that monocytes also have important functions in cytokine production ( Secombes 1991 ). Afiyanti et al . (2018) proposed that increased numbers of monocytes were associated with bacterial infection.…”

Section: Discussionmentioning

confidence: 99%

Hematological Evaluation of Three Common Teleosts in Relation to The Environmental Changes from Trang Province, Thailand

Archig Jeamah,

Sinlapachai Senarat,

Suparat Kongoh

et al. 2023

tlsr

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Hematological evaluation of fish is essential to the assessment of their physiological status. This study describes the morphometric analysis and comparison of blood cell characteristics in Zanarchopterus sp., Gerres filamentosus Cuvier, 1829 and Leiognathus decorus (De Vis, 1884). The species were collected at two locations off the coast of Trang Province, Thailand. A comparative hematological evaluation was made to assess the effects of environmental conditions on the blood of the fish. Ten individuals of each species were collected from a seagrass bed at Libong Island, where human activities are increasing, and from a secluded sandy beach. Their blood samples were analysed using the blood smear technique. Erythrocytes of all the studied fishes were either elliptical or oval. The morphometric data from both locations showed that erythrocytes were of similar size, except for those of Zanarchopterus sp. Fish from both stations showed several types of leukocytes, including neutrophils and lymphocytes.The highest proportion of leukocytes was made up of lymphocytes, followed by neutrophils. However, monocytes were only observed in fish from Libong Island and the erythrocytic nuclei of fish collected from Libong Island were both reniform and lobate. Our results show the potential of hematological evaluation as an early warning signal of environmental impacts on aquatic animals. The determination of baseline parameters could provide a tool for the monitoring of environmental quality.

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Leukocyte Count and Differential Leukocyte Count of Carp (Cyprinus carpio Linn) after Infected by Aeromonas salmonicida

Cited by 4 publications

References 0 publications

Haemato-Immunological Response of Immunized Atlantic Salmon (Salmo salar) to Moritella viscosa Challenge and Antigens

Haemato-Immunological Response of Immunized Atlantic Salmon (Salmo salar) to Moritella viscosa Challenge and Antigens

Trying to Use Antibiotics Alternatives to Raise Immune Efficiency and Growth Performance in Tilapia Nilotica

Hematological Evaluation of Three Common Teleosts in Relation to The Environmental Changes from Trang Province, Thailand

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