Leukocyte Depletion and Storage of Single‐Donor Platelet Concentrates

Weisbach, Volker; Putzo, Alexander; Zingsem, J.; Riewald, Matthias; Zimmermann, Robert; Eckstein, Reinhold; Riess, Hanno

doi:10.1046/j.1423-0410.1997.00020.x

Cited by 18 publications

(8 citation statements)

References 33 publications

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“…In this case, preSF of single–donor platelet apheresis improved the storage conditions thus leading to a more efficient use of TH. This is in contradiction to data presented recently on leukocyte depletion of single–donor platelet concentrates [28]. This discrepancy may be explained by the difference in WBC content at day 0 (one log more WBC per apheresis in our study as compared to that of Weisbach et al [28]) and by the different methods of preparation of single–donor PC in both studies, and also by the fact that the study of Weisbach et al [28]examined only 10 THs.…”

Section: Discussioncontrasting

confidence: 99%

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Benefit of Prestorage Leukocyte Depletion of Single–Donor Platelet Concentrates

et al. 1999

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Background and Objectives: The presence of contaminating white blood cells (WBCs) in platelet concentrates is associated with transfusion reactions and may adversely alter the quality of platelets during storage. Leukocyte depletion by filtration of platelets has been increasingly used to avoid these complications. However, the best time for filtration and the benefits of filtering single–donor platelet concentrates (thrombapheresis, TH) have yet to be clearly defined. Methods: In a randomized study of 202 TH collected with an Autopheresis C system, we determined whether prestorage filtration (preSF) of WBCs from TH as compared with poststorage (bedside) filtration (postSF) resulted in a better product. Levels of cytokines and C3a accumulating in the medium during storage, platelet activation state, in vivo platelet recovery, and transfusion reactions were compared in pre– and poststorage products. Results: As compared to preSF, significantly more postSF TH had detectable levels of tumor necrosis factor–α (TNF–α; 47 vs. 15%; p<0.0001) and interleukin 6 (13 vs. 3%; p = 0.02), lower pH (p<0.0001) and decreased levels of C3a (910 vs. 2,000 pg/ml; p<0.0001). Furthermore, platelet activation was increased in postSF TH (p = 0.022). PostSF TH tended to plug the bedside filter (27% of postSF TH delivered) from day 3 onward. There was also a significant difference in platelet recovery, postSF TH showing a lower corrected count increment (CCI; p = 0.0055) when taking into account the postSF TH that plugged filters (CCI = 0), but no difference when plugged TH were excluded. A correlation could be established between TNF–α levels and poor in vivo recovery (p<0.0001). Febrile nonhemolytic transfusion reactions were low in both groups (4 and 9%). Conclusion: These results indicate a benefit of preSF TH as compared with postSF TH based on the following parameters: decrease in cytokine levels, less platelet activation, maintenance of higher pH, and more efficient use of stored platelets (27% of postSF TH were lost because of plugging of filters). These results apply particularly to the Autopheresis C systems with its high initial WBC content.

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Section: Discussioncontrasting

confidence: 99%

“…These conclusions apply specifically to the Autopheresis C system with its high initial WBC content. They also argue for the promotion of the newer systems of plateletpheresis with lower WBC content [28, 30, 39]. …”

Section: Discussionmentioning

confidence: 99%

Benefit of Prestorage Leukocyte Depletion of Single–Donor Platelet Concentrates

et al. 1999

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“…The collection efficiency of the final system was slightly but significantly (2%) inferior to the standard procedure. The drop in platelet yield is much lower than the 10–81% platelet loss reported for fiber filtration [18, 19, 20, 21, 22, 23, 24, 25]. …”

Section: Discussionmentioning

confidence: 75%

“…This leukoreduction is usually done by filtration [17]. However, filtration leukoreduction of PC not onyl reduces the WBC count in the filtered product but also causes platelet losses of about 20% (10–81%) [18, 19, 20, 21, 22, 23]. It may even sacrifice the complete PC in the case of blocked filters [24, 25].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Platelet Apheresis Technique for the Preparation of Leukocyte-Reduced Platelet Concentrates

Zingsem¹,

Glaser²,

Weisbach³

et al. 1998

Vox Sanguinis

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Objectives: Reduction of the white blood cell (WBC) contamination in platelet concentrates (PC) protects patients from the immunological and infectious side effects of platelet transfusion caused by WBC. This can be done either by filtration of the PC or by improved apheresis techniques that yield WBC-poor preparations. Methods: To evaluate an improved technique for platelet collection, we carried out 201 separations in 89 healthy cytapheresis donors using the new COBE Spectra leukoreduction system (LRS) and compared the results with those of standard dual-needle separations obtained with the same cell separator. Results: A small but statistically significant difference was found in platelet collection efficiency in comparison with the standard non-LRS software procedures (LRS: 52.6 vs. 56.3% for the reference). However, median WBC contamination was only 0.01 × 10⁶ WBC per LRS product. This significant (p < 0.0005) improvement corresponds to a 10-fold reduction of WBC as compared with the standard dual-needle technique. Conclusions: The COBE Spectra LRS system produced PCs with a platelet collection efficiency nearly equal to previous techniques and with a residual WBC content satisfying even the most stringent criteria for WBC-depleted blood components. As this purity is achieved without important platelet loss, conventional fiber filtration no longer seems necessary in this kind of PC.

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“…[10][11][12][13][14] A literature search was also performed to identify studies that had reported ranges for the various test results performed on PLT products stored for 5 and/or 7 days. [15][16][17][18][19][20][21][22][23][24][25] The reported values in these studies were used as a reference range for comparative purposes.…”

Section: Laboratory Testingmentioning

confidence: 99%

In vitro evaluation of prestorage pooled leukoreduced whole blood–derived platelets stored for up to 7 days

Heddle

Barty²,

Sigouin³

et al. 2005

Transfusion

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Leukocyte Depletion and Storage of Single‐Donor Platelet Concentrates

Cited by 18 publications

References 33 publications

Benefit of Prestorage Leukocyte Depletion of Single–Donor Platelet Concentrates

Benefit of Prestorage Leukocyte Depletion of Single–Donor Platelet Concentrates

Evaluation of a Platelet Apheresis Technique for the Preparation of Leukocyte-Reduced Platelet Concentrates

In vitro evaluation of prestorage pooled leukoreduced whole blood–derived platelets stored for up to 7 days

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Leukocyte Depletion and Storage of Single‐Donor Platelet Concentrates

Cited by 18 publications

References 33 publications

Benefit of Prestorage Leukocyte Depletion of Single&ndash;Donor Platelet Concentrates

Benefit of Prestorage Leukocyte Depletion of Single&ndash;Donor Platelet Concentrates

Evaluation of a Platelet Apheresis Technique for the Preparation of Leukocyte-Reduced Platelet Concentrates

In vitro evaluation of prestorage pooled leukoreduced whole blood–derived platelets stored for up to 7 days

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Product

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Benefit of Prestorage Leukocyte Depletion of Single–Donor Platelet Concentrates

Benefit of Prestorage Leukocyte Depletion of Single–Donor Platelet Concentrates