Leukocyte depletion enhances cultured endothelial retention on vascular prostheses

Emerick, Scott; Herring, Malcolm B.; Arnold, Michael A.; Baughman, Sally; Reilly, Kathleen H.; Glover, John

doi:10.1067/mva.1987.avs0050342

Cited by 10 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1,2 We can hypothesize that the latter is caused by both expression of leukocyte adhesion molecules and production of cytokines by ECs activated by the underlying polymer surface.…”

Section: Introductionmentioning

confidence: 99%

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Zijpp

Poot

Feijén

2003

J Biomedical Materials Res

View full text Add to dashboard Cite

Small-diameter vascular grafts rapidly fail as a result of blood coagulation and platelet deposition. Endothelial cells lining the inner side of blood vessels can provide the graft lumen with an antithrombogenic surface. One of the remaining problems is cell detachment after restoration of blood flow, because of infiltration of leukocytes that respond to an inflammatory-like activation of the endothelial cells. This endothelial activation is possibly caused by the surface characteristics of the underlying polymer. To get more insight into the effects of the polymer surface on endothelial cell activation, we seeded human umbilical vein endothelial cells (HUVECs) in various densities and subsequently grew them on tissue culture polystyrene (TCPS; hydrophilic) and polystyrene (PS; hydrophobic) surfaces. To improve cell adhesion, surfaces were coated with purified fibronectin prior to cell seeding. During proliferation, the expressions of the leukocyte adhesion molecules ICAM-1 and VCAM-1 were determined. Results indicate that ICAM-1 expression is not influenced by the character of the polymer surface, and that VCAM-1 expression is slightly higher on the TCPS surface. Expressions of both adhesion molecules are influenced by the seeding density and time of proliferation. At low seeding densities (< or = 10,000 cells/cm(2)), a relatively low percentage of nonexogenously activated cells expressed ICAM-1 during the first 3 days of proliferation compared to higher seeding densities. Although less pronounced, this was also observed for the percentage of cells expressing VCAM-1. During proliferation, the amount of ICAM-1 per endothelial cell increased, whereas the expression of VCAM-1 remained low. The absence of large differences in leukocyte adhesion molecule expression by endothelial cells grown on TCPS or PS is possibly caused by coating of the surfaces with fibronectin. It is known that surface hydrophilicity influences protein adsorption. Although this had no or little effect on leukocyte adhesion molecule expression, endothelial cell growth was affected, because proliferation was slower on the hydrophobic PS.

show abstract

“…1,2 We can hypothesize that the latter is caused by both expression of leukocyte adhesion molecules and production of cytokines by ECs activated by the underlying polymer surface.…”

Section: Introductionmentioning

confidence: 99%

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Zijpp

Poot

Feijén

2003

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Whereas control grafts exhibited occasional white cells, the density of white cells on bare areas of sodded graft surfaces was significantly different. Herring et al 19 and Emerick et al 20 indicate that significant activation and attraction of leukocytes occur after implantation of endothelialized prosthetic grafts. We therefore suggest that a major factor in the early healing of endothelial cell transplanted grafts may be the sequestration of leukocytes to areas of graft that have not been completely endothelialized.…”

Section: Discussionmentioning

confidence: 99%

Microvascular endothelial cell sodding of 1-mm expanded polytetrafluoroethylene vascular grafts.

Ahlswede

Williams

1994

Arterioscler Thromb

View full text Add to dashboard Cite

The formation of an endothelial cell lining on the inner surface of polymeric grafts may reduce the inherent thrombogenicity of synthetic implants. Endothelial cell transplantation onto the luminal surface of grafts has been suggested as one method of creating new endothelial cell linings on grafts. The purpose of this study was to morphologically evaluate the very early events of healing (between 4 and 14 days) of 1-mm-internal-diameter expanded polytetrafluoroethylene (ePTFE) grafts that were treated with autologous microvessel endothelial cells at the time of graft implantation. We evaluated the development of new intimal linings in microvascular endothelial cell-sodded 1-mm ePTFE vascular grafts and compared their healing characteristics with noncell-treated grafts by using a rat aortic graft model. Endothelial cells were isolated from intraperitoneal fat pads of female rats and transplanted onto the grafts by using a pressure sodding method. One-centimeter-long grafts were immediately implanted as interpositional grafts in the aorta. Non-celltreated grafts were also implanted. Grafts were explanted 4, 7, and 14 days after implantation and were evaluated by light and scanning electron microscopy. Morphometric analysis of the graft surfaces revealed the cellular coverage in sodded grafts to be 93.7±8.7% and in nonsoddcd grafts, 1.1±1.9%. Areas not covered by cells exhibited thrombus and bare graft. The luminal lining of cells exhibited morphological characteristics, indicating they were antithrombogenic, based on morphological criteria, and exhibited characteristics of endothelium. We conclude that microvascular endothelial cell sodding of microvascular ePTFE grafts results in a dramatic acceleration of the formation of a luminal cell lining that covers more than 90% of the luminal surface of grafts by 7 days. While control grafts exhibited a thrombogenic surface, sodded grafts exhibited an antithrombogenic cell lining, as indicated by the absence of adherent platelets, white cells, and fibrin. (Arterioscler Thromb. 1994;14:25-31.)

show abstract

“…Since our own investigations have also shown no significant effect on endothelial leukocyte adhesion molecule expression during EC culture on an artificial surface, it is our belief that the activated ECs found in implanted grafts (Emerick & Herring, 1989) are stimulated by the presence of an 'external' endothelial activator in the blood or the surrounding tissue after implantation of the endothelialized artificial graft. Judged by the finding that ECs attached to an artificial surface can still respond to an activating stimulus, this activator could very well be a cytokine.…”

Section: Endothelial Activationmentioning

confidence: 98%

“…In vivo experiments have shown that loss of seeded endothelial cells is not simply due to shear forces associated with blood flow, but also indicate that massive leukocyte (white blood cell) infiltration is involved in endothelial cell detachment (Emerick et al, 1989).…”

Section: Endothelialization Of Small-diameter Vascular Prosthesesmentioning

confidence: 99%

Endothelialization of Small-Diameter Vascular Prostheses

Zijpp¹,

A.A.²,

Feijen³

2003

Archives of Physiology and Biochemistry

View full text Add to dashboard Cite

In the field of arterial vascular reconstructions there is an increasing need for functional small-diameter artificial grafts (inner diameter < 6 mm). When autologous replacement vessels are not available, for example because of the bad condition of the vascular system in the patient, the surgeon has no other alternative than to implant a synthetic polymerbased vessel. After implantation the initial major problem concerning these vessels is the almost immediate occlusion, due to blood coagulation and platelet deposition, under the relatively low flow conditions. As the search for the perfect bio-inert polymer has not revealed a material with suitable properties for this application, improved performance of small-diameter artificial blood vessels is now being sought in the biological field. The poor blood-compatibility of an artificial vascular graft is not simply because of its coagulation-stimulating or platelet-activating properties, but more due to its inability to actively participate in the prevention of blood coagulation and platelet deposition. As these functions are naturally performed by endothelial cells, the utilization of these cells seems inevitable for the construction of a functional small-diameter artificial blood vessels. This review describes the current status of the use of endothelial cells to improve the performance of artificial vascular prostheses.

show abstract

Leukocyte depletion enhances cultured endothelial retention on vascular prostheses

Cited by 10 publications

References 0 publications

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Microvascular endothelial cell sodding of 1-mm expanded polytetrafluoroethylene vascular grafts.

Endothelialization of Small-Diameter Vascular Prostheses

Contact Info

Product

Resources

About