Leukocyte Distribution in the Open Eye Tears of Normal and Dry Eye Subjects

Postnikoff, Cameron; Huisingh, Carrie; McGwin, Gerald; Nichols, Kelly K.

doi:10.1080/02713683.2018.1500611

Cited by 18 publications

(22 citation statements)

References 30 publications

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“…In this study, human subjects collected samples of sterile saline rinse applied to the ocular surface upon awakening in the morning. Using saline eye wash to collect microbiome samples is novel and less invasive than swabs of the ocular surface that has previously been used for the collection of tear leukocytes 18,20 . In a series of pooled samples from both eyes, the closed eye tear microbiome in individuals with dry eye disease was distinct from that in healthy controls.…”

Section: Discussionmentioning

confidence: 99%

“…The evidence may also suggest the immune system of the closed eye cannot regulate the ocular surface microbiome during sleep. In the closed eye, preliminary evidence suggests higher numbers of neutrophils with an enhanced degranulation response in dry eye disease 18,20 , indicating a dysregulated immune response. This dysregulation may permit increased microbial diversity.…”

Section: Discussionmentioning

confidence: 99%

“…Subjects were trained on the first visit to perform a daily eye wash. Briefly, as described previously 18 , 39 , 40 , subjects were provided one 50 mL sterile centrifuge tube, and two 10 mL sterile polypropylene syringes (BD Biosciences; San Jose, CA, USA) with 5 mL of sterile saline per syringe. Immediately after awakening, subjects were instructed to wash their eyes by dispensing the saline gently across the ocular surface, with the runoff collected in a sterile Eppendorf tube.…”

Section: Methodsmentioning

confidence: 99%

“…The closed dry eye microbiome remains distinct despite daily eye wash. Daily eye wash on awakening with sterile saline is a proposed therapy for dry eye disease 18 . We tested if the microbial community composition could be normalized by the prescription of daily saline eye wash.…”

Section: Cohortmentioning

confidence: 99%

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The closed eye harbours a unique microbiome in dry eye disease

Willis

Postnikoff

Freeman

et al. 2020

Sci Rep

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Dry eye affects millions of individuals. In experimental models, dry eye disease is associated with T helper cell 17-mediated inflammation of the ocular surface that may cause persistent damage to the corneal epithelium. However, the initiating and perpetuating factors associated with chronic inflammation of the ocular surface remain unclear. The ocular microbiota alters ocular surface inflammation and may influence dry eye disease development and progression. Here, we collected serial samples of tears on awakening from sleep, closed eye tears, during a randomized clinical trial of a non-pharmaceutical dry eye therapy and used 16S rRNA metabarcoding to characterize the microbiome. We show the closed dry eye microbiome is distinct from the healthy closed eye microbiome, and that the microbiome remains distinct despite daily saline eye wash upon awakening. The ocular microbiome was described only recently, and this report implicates a distinct microbiome in ocular disease development. Our findings suggest an interplay between microbial commensals and inflammation on the ocular surface. This information may inform future studies of the pathophysiological mechanisms of dry eye disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Cohortmentioning

confidence: 99%

See 2 more Smart Citations

The closed eye harbours a unique microbiome in dry eye disease

Willis

Postnikoff

Freeman

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, human subjects collected samples of sterile saline rinse applied to the ocular surface upon awakening in the morning. Using saline eye wash to collect microbiome samples is novel and less invasive than swabs of the ocular surface, which has been routinely used for the collection of tear leukocytes 18,20 . In a series of pooled samples from both eyes, the closed eye tear microbiome in individuals with dry eye disease was distinct from that in healthy controls.…”

Section: Discussionmentioning

confidence: 99%

The closed eye harbors a unique microbiome in dry eye disease

Willis

Postnikoff

Freeman

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Dry eye affects millions of individuals. In experimental models, dry eye disease is associated with T helper cell 17-mediated inflammation of the ocular surface that may cause persistent damage to the corneal epithelium. However, the initiating and perpetuating factors associated with chronic inflammation of the ocular surface remain unclear. The ocular microbiota alters ocular surface inflammation and may influence dry eye disease development and progression. Here, we collected serial samples of closed eye tears during a randomized clinical trial of a non-pharmaceutical dry eye therapy and used 16S rRNA metabarcoding to characterize the microbiome. We show the closed dry eye microbiome is distinct from the healthy closed eye microbiome. The ocular microbiome was described only recently, and this report implicates a distinct microbiome in ocular disease development. Our findings suggest an interplay between microbial commensals and inflammation on the ocular surface. This information may inform future studies of the pathophysiological mechanisms of dry eye disease.

show abstract

Hue and Cry

Prashar¹

2019

Shed Tears for Diagnostics

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Leukocyte Distribution in the Open Eye Tears of Normal and Dry Eye Subjects

Cited by 18 publications

References 30 publications

The closed eye harbours a unique microbiome in dry eye disease

The closed eye harbours a unique microbiome in dry eye disease

The closed eye harbors a unique microbiome in dry eye disease

Hue and Cry

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