Leukocyte-endothelial interactions within the ocular microcirculation in inflammation and infection

Al-Banna, Nadia; Toguri, James Thomas; Kelly, M. E.; Lehmann, Christian

doi:10.3233/ch-131780

Cited by 8 publications

(3 citation statements)

References 102 publications

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“…NO and platelet-activating factor (PAF)] are released. [1][2][3][4][5] These activities are intended to restore homeostasis in the body, which depends on the relevant repair processes in damaged tissues. Therefore, the reasons for the development of many chronic lesions, such as infective endocarditis (IE), diabetic foot ulcerations (DFU) or chronic wound infections (CWI), include both microbial infection and pathological changes within the host tissue, which disturb these physiological repair processes.…”

Section: Introductionmentioning

confidence: 99%

The immunomodulatory potential of Leonurus cardiaca extract in relation to endothelial cells and platelets

et al. 2017

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The immunomodulatory activity of Leonurus cardiaca L. polyphenol-rich extract (LCE) was tested in vitro on HUVECs to explore its potential therapeutic usefulness in the treatment of inflammatory lesions. The phytochemical composition of LCE, its antioxidant and cytotoxic activity, and the influence of LCE on NO and platelet-activating factor (PAF) secretion by HUVECs and platelet aggregation were all assessed. Total polyphenol contents in LCE reached 137.0 ± 0.8 mg/g, with hydroxycinnamic acid derivatives as the predominant phenolic compounds. LCE expressed antioxidant capacity, which was, however, 13- to 16-fold lower than the antioxidant activity of ascorbic acid. The plant extract was not cytotoxic up to a concentration 4500 µg/ml and did not exhibit proapoptotic activity. LCE significantly increased NO production in HUVECs in a concentration-dependent manner and led to the inhibition of PAF secretion induced by staphylococcal peptidoglycan. The extract used at the concentration of 100 µg/ml significantly reduced platelet aggregation in the presence of arachidonic acid. We provide in vitro data demonstrating the immunomodulatory potential of LCE, which may be beneficial in preventing the development of difficult-to-treat inflammatory lesions within chronically infected tissues.

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Section: Introductionmentioning

confidence: 99%

The immunomodulatory potential of Leonurus cardiaca extract in relation to endothelial cells and platelets

et al. 2017

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“…It was consisted with previous reports that LPS injury tissue and led cell necroptosis ( Li et al, 2016 ; Huang et al, 2019 ). In fact, LPS action on endothelial cells is intend to occur an inflammation within endothelial-neutrophil interactions ( Al-Banna et al, 2013 ; Shi et al, 2014 ), and IL-1 family is closely related with inflammation ( Dinarello, 2011 ; Liu et al, 2016 ; Gunther et al, 2017 ). In addition, we found that LPS damaged the transendothelial neutrophils and led the bacterial escape from cells ( Figure 1C ).…”

Section: Resultsmentioning

confidence: 99%

Endothelial-Derived Interleukin-1α Activates Innate Immunity by Promoting the Bactericidal Activity of Transendothelial Neutrophils

Liu

Zhang

et al. 2020

Front. Cell Dev. Biol.

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Migration of neutrophils across endothelial barriers to capture and eliminate bacteria is served as the first line of innate immunity. Bacterial virulence factors damage endothelium to produce inflammatory cytokines interacts with neutrophils. However, the mechanisms that behind endothelial-neutrophil interaction impact on the bactericidal activity remain unclear. Therefore, we aimed to find the target proteins on endothelial cells that triggered the bactericidal activity of transendothelial neutrophils. Herein, we built the infected models on rats and endothelial-neutrophil co-cultural system (Transwell) and discovered that endothelial-derived IL-1α promoted the survival of rats under Escherichia coli infection and enhanced the bactericidal activity of transendothelial neutrophils in vivo and in vitro. Results further showed that IL-1α was inhibited by lipopolysaccharide (LPS) in the endothelial-neutrophil interaction. We found that LPS mainly damaged cell membrane and induced cell necrosis to interrupt neutrophil migration from endothelial barrier. Thus, we used the isobaric tags for relative and absolute quantification (iTRAQ) method to identify different proteins of endothelial cells. Results showed that IL-1α targeted cellular plasma membrane, endoplasmic reticulum and mitochondrial envelope and triggered eleven common proteins to persistently regulate. During the early phase, IL-1α triggered the upregulation of cell adhesion molecules (CAMs) to promote neutrophil adhesion, while oxidative phosphorylation was involved in long time regulation to induce transmigration of neutrophils against bacteria. Our results highlight the critical mechanism of endothelial-derived IL-1α on promoting bactericidal activity of transendothelial neutrophils and the findings of IL-1α triggered proteins provide the potentially important targets on the regulation of innate immunity.

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“…The most common methods employed to evaluate the microcirculation of different tissues and organs are laser Doppler flowmetry, orthogonal polarization spectral imaging, side stream dark field imaging (SDF) and IVM (Černý et al ., ; Al‐Banna et al ., ; Eriksson et al ., ; Small et al ., ). The use of SDF technology as reported by our group has shown to be a useful tool to examine the iridial microcirculation (IMIC) in rats (Cerny et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of iris functional capillary density in experimental local and systemic inflammation

Arora

Islam

Wafa

et al. 2017

Journal of Microscopy

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The ocular microcirculation represents an important target to treat inflammatory diseases of eye, where impairment of microvascular blood flow plays key role as, for example, in anterior uveitis. To evaluate novel interventions targeting the microcirculation, appropriate and reliable tools to study this particular microvascular bed are needed. Intravital microscopy (IVM) belongs to several methods allowing evaluation of microcirculation experimentally, even in small animals. The aim of our study was to examine the iridial microcirculation (IMIC) in uveitis induced by local or systemic endotoxin administration in rats and mice by IVM and to propose new parameters to quantify the changes within the IMIC. Systemic inflammation was induced in rats by intravenous endotoxin administration, control group received normal saline intravenously. Local inflammation was induced in mice by intravitreal endotoxin administration, the control group received normal saline intravitreally. IVM of IMIC was performed in animals receiving systemic endotoxin prior injection and 1 and 2 h afterwards, respectively, in animals receiving intravitreal endotoxin/saline prior local injection and 5 h afterwards. Obtained video recordings were analyzed off-line. Functional capillary density (FCD) and dysfunctional capillary density (DCD) were evaluated for description of IMIC, and calculation of FCD/DCD ratio was performed. In systemic inflammation, FCD was significantly decreased compared to control animals. In local inflammation, the number of functional capillaries in the IMIC was significantly reduced following the endotoxin challenge. Analysis of the DCD revealed a significant increase in capillaries with reduced perfusion after intravitreal endotoxin administration and right shift of the FCD/DCD ratio was observed after endotoxin local injection. Detecting and quantifying changes in IMIC during systemic or local inflammation in experimental animals by IVM was feasible. Therefore, IVM of the IMIC represents a valuable tool to evaluate and quantify inflammatory changes in experimental eye disease.

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Leukocyte-endothelial interactions within the ocular microcirculation in inflammation and infection

Cited by 8 publications

References 102 publications

The immunomodulatory potential of Leonurus cardiaca extract in relation to endothelial cells and platelets

The immunomodulatory potential of Leonurus cardiaca extract in relation to endothelial cells and platelets

Endothelial-Derived Interleukin-1α Activates Innate Immunity by Promoting the Bactericidal Activity of Transendothelial Neutrophils

Evaluation of iris functional capillary density in experimental local and systemic inflammation

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