Leukocyte Migration in Adipose Tissue of Mice Null for ICAM‐1 and Mac‐1 Adhesion Receptors

Robker, Rebecca L.; Collins, Robert G.; Beaudet, Arthur L.; Mersmann, Harry J.; Smith, C. Wayne

doi:10.1038/oby.2004.114

Cited by 63 publications

(59 citation statements)

References 22 publications

(19 reference statements)

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“…4A). Region 1 contained lymphocytes consistent with the previously published results (7,32). ICAM-1 expression in this region was low, compared with cells in regions 2 and 3 (Fig.…”

Section: Resultssupporting

confidence: 92%

“…This is the first demonstration of relatively rapid changes in a specific population of macrophages in adipose tissue, corroborating longer term studies that have correlated increases in adiposity with increased F4/80ϩ macrophage infiltration into adipose tissue (32,50,53). Only the F4/80ϩ populations expressing CD11c and CD14 increase in response to a high-fat diet.…”

Section: Discussionsupporting

confidence: 82%

“…Of interest to the current study is the finding that macrophages accumulate in adipose tissue of animals on a high-fat diet in a sex-dependent fashion (32,50). In those studies, macrophages in adipose tissue were primarily identified by expression of F4/80 (22).…”

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confidence: 95%

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ICAM-1 expression in adipose tissue: effects of diet-induced obesity in mice

Brake

Smith

Mersmann

et al. 2006

American Journal of Physiology-Cell Physiology

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137

107

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“…4A). Region 1 contained lymphocytes consistent with the previously published results (7,32). ICAM-1 expression in this region was low, compared with cells in regions 2 and 3 (Fig.…”

Section: Resultssupporting

confidence: 92%

Section: Discussionsupporting

confidence: 82%

See 1 more Smart Citation

ICAM-1 expression in adipose tissue: effects of diet-induced obesity in mice

Brake

Smith

Mersmann

et al. 2006

American Journal of Physiology-Cell Physiology

Self Cite

137

107

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show abstract

“…Previous reports showed that the expression of adhesion molecules in adipose tissue was increased in the obese condition [5,6] and a different study indicated that HFD induced obese mice exhibited higher expression of Intercellular adhesion molecule 1(ICAM-1) and Vascular cell adhesion [7]. Thus, adhesion molecules affect obesity-induced macrophage infiltration into adipose tissue.…”

Section: Introduction *mentioning

confidence: 97%

The effects of either resveratrol or exercise on macrophage infiltration and switching from M1 to M2 in high fat diet mice

Jeong

Lee

Park

et al. 2015

J. Exerc. Nutr, Biochem.

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The effects of either resveratrol or exercise on macrophage infiltration and switching from M1 to M2 in high fat diet mice. JENB., Vol. 19, No. 2, pp.65-72, 2015 [Purpose] The aim of this study was to compare the effectiveness of either resveratrol supplementation or exercise training on macrophage infiltration and switching from M1 to M2 kupffer cells in high fat diet mice.[Methods] C57BL/6 mice were separated into 5 groups: normal diet (ND; n = 6), high-fat diet (HD; n = 6), high-fat diet with resveratrol (HR; n = 6), high-fat diet with exercise (HE; n = 6) or high-fat diet with resveratrol and exercise (HRE; n = 6). Resveratrol supplementation mice were orally gavaged with resveratrol (25mg/kg of body weight) dissolved in 50% propylene glycol. Exercise mice ran on a treadmill at 12-20 m/min for 30-60 min/day, 5 times/week for 12 weeks.[Results] After 12 weeks of intervention, the liver was analyzed. F4/80 expression was evaluated by western blot while CD11c and CD163 mRNA expressions were evaluated by RT-PCR. The weights of the body and liver were significantly increased in the HD and HR group compared to the ND group (p < 0.01). However, the weights were most effectively reduced in the HE and HRE groups compared to the HD group (p < 0.05). The macrophage marker, F4/80 expression was significantly lower in the HE and HRE groups compared to the HD group (p < 0.05). mRNA expression of the M1 macrophage marker, CD11c, in the HD group was significantly increased compared to the ND group (p < 0.01). mRNA expression of the M2 macrophage specific marker, CD163, in the HE and HRE groups were significantly increased compared to the HD group (p < 0.05). The mRNA expressions of TLR4, ICAM-1 and VCAM-1, which induce pro-inflammatory cytokine production, were strongly decreased in the HR, HE, and HRE groups compared to the HD group.[Conclusion] These results suggest that moderate exercise training inhibits macrophage infiltration and up regulation of CD163 expression. However, resveratrol supplementation is not enough to ameliorate obesity-induced macrophage infiltration and switching.

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“…Furthermore, the number of leukocytes in the adipose tissue of ICAM-1-deficient mice and Mac-1-deficient mice fed HFD were the same as in the WT mice fed HFD. Consequently, these adhesion receptors are not required for leukocyte migration into adipose tissue (34).…”

Section: Discussionmentioning

confidence: 99%

P-selectin glycoprotein ligand-1 deficiency is protective against obesity-related insulin resistance

Sato¹

2011

Okayama I. Z.

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OBJECTIVE-An inflammatory process is involved in the mechanism of obesity-related insulin resistance. Recent studies indicate that monocyte chemoattractant protein-1 (MCP-1) is a major chemokine that promotes monocyte infiltration into adipose tissues; however, the adhesion pathway in adipose tissues remains unclear. We aimed to clarify the adhesion molecules that mediate monocyte infiltration into adipose tissue. RESEARCH DESIGN AND METHODS-We used a DNA microarray to compare the gene expression profiles in epididymal white adipose tissues (eWAT) between db/db mice and C57/BL6 mice each fed a high-fat diet (HFD) or a low-fat diet (LFD). We investigated the change of insulin resistance and inflammation in eWAT in P-selectin glycoprotein ligand-1 (PSGL-1) homozygous knockout (PSGL-1 Ϫ/Ϫ ) mice compared with wild-type (WT) mice fed HFD.RESULTS-DNA microarray analysis revealed that PSGL-1, a major ligand for selectins, is upregulated in eWAT from both db/db mice and WT mice fed HFD. Quantitative real-time RT-PCR and immunohistochemistry showed that PSGL-1 is expressed on both endothelial cells and macrophages in eWAT of obese mice. PSGL-1 Ϫ/Ϫ mice fed HFD showed a remarkable reduction of macrophage accumulation and expression of proinflammatory genes, including MCP-1 in eWAT. Moreover, adipocyte hypertrophy, insulin resistance, lipid metabolism, and hepatic fatty change were improved in PSGL-1 Ϫ/Ϫ mice compared with WT mice fed HFD.CONCLUSIONS-These results indicate that PSGL-1 is a crucial adhesion molecule for the recruitment of monocytes into adipose tissues in obese mice, making it a candidate for a novel therapeutic target for the prevention of obesity-related insulin resistance.

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Leukocyte Migration in Adipose Tissue of Mice Null for ICAM‐1 and Mac‐1 Adhesion Receptors

Cited by 63 publications

References 22 publications

ICAM-1 expression in adipose tissue: effects of diet-induced obesity in mice

ICAM-1 expression in adipose tissue: effects of diet-induced obesity in mice

The effects of either resveratrol or exercise on macrophage infiltration and switching from M1 to M2 in high fat diet mice

P-selectin glycoprotein ligand-1 deficiency is protective against obesity-related insulin resistance

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