Leukocyte-platelet-rich plasma (L-PRP) impairs the osteoconductive capacity of the autograft associated to changes in the immunolocalization of TGF-β1 and its co-expression with Wnt10b and CD34 cells

Giovanini, Allan Fernando; Deliberador, Tatiana Miranda; Nemeth, José Eduardo Tannuri; Crivellaro, Viviane Rozeira; Portela, Germán; Filho, Marco Antonio de Oliveira; Araújo, Maria Christina Barbosa de; Zielak, João César; Gonzaga, Carla Castiglia

doi:10.1016/j.jcms.2013.01.003

Cited by 15 publications

(7 citation statements)

References 48 publications

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“…Several studies have supported leukocyte-rich PRP, arguing that the WBC potentiate the release of cytokines from platelets to improve healing, and also confers antimicrobial properties to reduce infection rates, as demonstrated in vitro [4,15,24,25]. Others argue that the release of these cytokines causes a highly inflammatory reaction, predisposing to fibrosis and structurally weaker tissue, while the purported antimicrobial effects have not been noted in in vivo studies [1,11,16,21,30]. Additionally, as it pertains to intra-articular use, leukocyte-rich PRP has been shown to cause increased post-injection pain, cell death, and synoviocyte activation than leukocyte-poor PRP [2].…”

Section: Discussionmentioning

confidence: 99%

Commercial Separation Systems Designed for Preparation of Platelet-Rich Plasma Yield Differences in Cellular Composition

Degen

Bernard

Oliver³

et al. 2017

HSS Journal®: The Musculoskeletal Journal of Hospital for Speci

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Section: Discussionmentioning

confidence: 99%

Commercial Separation Systems Designed for Preparation of Platelet-Rich Plasma Yield Differences in Cellular Composition

Degen

Bernard

Oliver³

et al. 2017

HSS Journal®: The Musculoskeletal Journal of Hospital for Speci

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“…The inclusion of leukocytes within platelet rich plasma products has always been a matter of debate. The deleterious effects of leukocytes have been widely described [ 31 – 34 ]. In contrast to platelets, white blood cells are mainly considered to contain and produce inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

Leukocyte Inclusion within a Platelet Rich Plasma-Derived Fibrin Scaffold Stimulates a More Pro-Inflammatory Environment and Alters Fibrin Properties

et al. 2015

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One of the main differences among platelet-rich plasma (PRP) products is the inclusion of leukocytes that may affect the biological efficacy of these autologous preparations. The purpose of this study was to evaluate whether the addition of leukocytes modified the morphological, biomechanical and biological properties of PRP under normal and inflammatory conditions. The release of pro-inflammatory cytokines from plasma rich in growth factors (PRGF) and leukocyte-platelet rich plasma (L-PRP) scaffolds was determined by enzyme-linked immunosorbent assay (ELISA) and was significantly increased under an inflammatory condition when leukocytes were included in the PRP. Fibroblasts and osteoblasts treated with L-PRP, under an inflammatory situation, underwent a greater activation of NFĸB pathway, proliferated significantly less and secreted a higher concentration of pro-inflammatory cytokines. These cellular events were assessed through Western blot and fluorimetric and ELISA methods, respectively. Therefore, the inclusion of leukocytes induced significantly higher pro-inflammatory conditions.

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“…Few studies evaluated the use of L‐PRP for the regeneration of calvarial bone defects showing that it may be unfavourable for craniofacial bone repair (Cerci, Portela, Cunha, Grossi & Zielak, ; Giovanini et al., , ; Portela, Cerci, Pedrotti, Araujo & Deliberador, ; Yin, Qi, Zhang, Sheng & Xu, ).…”

Section: Resultsmentioning

confidence: 99%

The use of bioactive factors to enhance bone regeneration: A narrative review

Donos

Dereka

Calciolari

2019

J Clinic Periodontology

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Aim This review critically appraises the available knowledge on the pre‐clinical and clinical use of bioactive factors for bone regeneration in the cranial and maxillofacial area. Materials and Methods The use of growth factors, amelogenins and autologous platelet concentrates (APCs) for bone regeneration was reviewed in a systematic manner. More specifically, pre‐clinical and clinical studies on ridge preservation, alveolar ridge augmentation, regeneration of peri‐implant defects and sinus augmentation models were considered. Results Amongst different bioactive factors, the highest pre‐clinical and clinical evidence of a positive effect on bone formation is associated with rhBMP‐2 and the lowest with amelogenins. While APCs seem to accelerate clinical healing and reduce postoperative discomfort, there is insufficient and contrasting evidence of a significant effect on hard tissue regeneration for the different clinical applications. Conclusions Although there is increasing evidence that bioactive factors might enhance the bone regeneration process, the great heterogeneity of the available studies and the limited number of RCTs do not allow to draw robust conclusions. Issues that still need to be investigated include the optimal carriers for bioactive agents (direct vs. indirect), the dosage, the timing of administration, as well as the possibility of combining different agents to promote synergistic effects.

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Leukocyte-platelet-rich plasma (L-PRP) impairs the osteoconductive capacity of the autograft associated to changes in the immunolocalization of TGF-β1 and its co-expression with Wnt10b and CD34 cells

Cited by 15 publications

References 48 publications

Commercial Separation Systems Designed for Preparation of Platelet-Rich Plasma Yield Differences in Cellular Composition

Commercial Separation Systems Designed for Preparation of Platelet-Rich Plasma Yield Differences in Cellular Composition

Leukocyte Inclusion within a Platelet Rich Plasma-Derived Fibrin Scaffold Stimulates a More Pro-Inflammatory Environment and Alters Fibrin Properties

The use of bioactive factors to enhance bone regeneration: A narrative review

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