Leukocyte-Poor CPD Blood

Abstract

A simple method is described for the preparation of leukocyte-poor red cell concentrates from fresh and stored CPD blood. Warm normal CPD blood is passed through a nylon wool filter. After centrifugation at 1,500g for 3 min, the plasma, the buffy coat and the top 30 ml of the red cell concentrate are eliminated. The average removal of leukocytes is better than 97%, the relation of remaining lymphocytes and granulocytes being 1:1. Red cell loss ranges around 20%. Advantages and disadvantages of the preparation are discussed.