Leukocyte reduction filters as an alternative source of peripheral blood leukocytes for research

Ferdowsi, Shirin; Abbasi-Malati, Zahra; Pourfathollah, Ali Akbar

doi:10.1016/j.htct.2020.10.963

Cited by 7 publications

(4 citation statements)

References 50 publications

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“…15,19 Leukoreduction (LR) is also an alternative to transfused blood components, the use of LR aims to reduce transfusion reactions by not only filtering leukocytes, but also filtering proinflammatory mediators in whole blood cells (WBC) used. 20,21…”

Section: Results Autoimmune Hemolytic Anemiamentioning

confidence: 99%

Blood Transfusion Therapy in Patients with Autoimmune Hemolytic Anemia

Irma

2022

bul.farmatera

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Management therapy of autoimmune hemolytic anemia (AIHA) with severe clinical symptoms requires supportive therapy such as blood components transfusion to improve hemodynamic conditions. This literature study aims to explain the role of blood transfusion therapy in AIHA cases. The method used is through searching articles in national and international journals published in the last 10 years that discuss transfusion in AIHA cases using the Google Scholar application. From the search results, it can be concluded that blood transfusion is a supportive therapy in cases of AIHA with symptoms of severe hypoxemia that interfere with hemodynamics and work function of body organ systems and findings of Hb levels < 7 mg/dL with the selection of blood components in the form of packed red cells (PRC), washed packed red cells (WPRC) and Leucoreduction (LR).

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Section: Results Autoimmune Hemolytic Anemiamentioning

confidence: 99%

Blood Transfusion Therapy in Patients with Autoimmune Hemolytic Anemia

Irma

2022

bul.farmatera

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“…To reduce transfusion-associated reactions, leukocytes are removed from donated blood with leucocyte reduction filters, which are a useful source of leukocytes for research [17]. In the Department of Transfusion Medicine in the Universiti Malaya Medical Centre in Kuala Lumpur, Malaysia, units of 400–450 ml donated blood are leukofiltered through Composelect 4F in-line leukoreduction filters (Fresenius HemoCare, Germany).…”

Section: Methodsmentioning

confidence: 99%

Replication and innate immune responses of two chikungunya virus genotypes in human monocyte-derived macrophages

Lau

Chua

Chan

et al. 2023

Journal of General Virology

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Chikungunya virus (CHIKV) is a re-emerging mosquito-borne virus, which causes epidemics of fever, joint pain and rash. There are three genotypes: West African, East/Central/South/Africa (ECSA) and Asian, with the latter two predominant globally. Genotype-specific differences in clinical presentations, virulence and immunopathology have been described. Macrophages are key cells in immune responses against CHIKV. Circulating blood monocytes enter tissue to differentiate into monocyte-derived macrophages (MDMs) in response to CHIKV infection at key replication sites such as lymphoid organs and joints. This study analyses differences in replication and induced immune mediators following infection of MDMs with Asian and ECSA CHIKV genotypes. Primary human MDMs were derived from residual blood donations. Replication of Asian (MY/06/37348) or ECSA (MY/08/065) genotype strains of CHIKV in MDMs was measured by plaque assay. Nineteen immune mediators were measured in infected cell supernatants using multiplexed immunoassay or ELISA. MY/08/065 showed significantly higher viral replication at 24 h post-infection (h p.i.) but induced significantly lower expression of proinflammatory cytokines (CCL-2, CCL-3, CCL-4, RANTES and CXCL-10) and the anti-inflammatory IL-1Ra compared to MY/06/37348. No differences were seen at later time points up to 72 h p.i. During early infection, MY/08/065 induced lower proinflammatory immune responses in MDMs. In vivo, this may lead to poorer initial control of viral infection, facilitating CHIKV replication and dissemination to other sites such as joints. This may explain the consistent past findings that the ECSA genotype is associated with greater viremia and severity of symptoms than the Asian genotype. Knowledge of CHIKV genotype-specific immunopathogenic mechanisms in human MDMs is important in understanding of clinical epidemiology, biomarkers and therapeutics in areas with co-circulation of different genotypes.

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“…Due to technical reasons, LRSCs used to isolate buffy coats in this study were stored overnight at 4 • C before processing. The advantage of leukapheresis compared to whole-blood samples is the large yield and fast isolation of mononuclear cells, allowing one to test for largescale experimental research setups [45]. All donors gave their written informed consent before the leukocyte reduction chambers were used for MNC isolation.…”

Section: Study Samplesmentioning

confidence: 99%

Quantitative and Functional Assessment of the Influence of Routinely Used Cryopreservation Media on Mononuclear Leukocytes for Medical Research

Haider

Hoberstorfer

Salzmann

et al. 2022

IJMS

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Quantitative and functional analysis of mononuclear leukocyte populations is an invaluable tool to understand the role of the immune system in the pathogenesis of a disease. Cryopreservation of mononuclear cells (MNCs) is routinely used to guarantee similar experimental conditions. Immune cells react differently to cryopreservation, and populations and functions of immune cells change during the process of freeze–thawing. To allow for a setup that preserves cell number and function optimally, we tested four different cryopreservation media. MNCs from 15 human individuals were analyzed. Before freezing and after thawing, the distribution of leukocytes was quantified by flow cytometry. Cultured cells were stimulated using lipopolysaccharide, and their immune response was quantified by flow cytometry, quantitative polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay (ELISA). Ultimately, the performance of the cryopreservation media was ranked. Cell recovery and viability were different between the media. Cryopreservation led to changes in the relative number of monocytes, T cells, B cells, and their subsets. The inflammatory response of MNCs was altered by cryopreservation, enhancing the basal production of inflammatory cytokines. Different cryopreservation media induce biases, which needs to be considered when designing a study relying on cryopreservation. Here, we provide an overview of four different cryopreservation media for choosing the optimal medium for a specific task.

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Leukocyte reduction filters as an alternative source of peripheral blood leukocytes for research

Cited by 7 publications

References 50 publications

Blood Transfusion Therapy in Patients with Autoimmune Hemolytic Anemia

Blood Transfusion Therapy in Patients with Autoimmune Hemolytic Anemia

Replication and innate immune responses of two chikungunya virus genotypes in human monocyte-derived macrophages

Quantitative and Functional Assessment of the Influence of Routinely Used Cryopreservation Media on Mononuclear Leukocytes for Medical Research

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