Leukocyte Rheology Before and After Chemotactic Activation in some Venous Diseases

Caimi, Gregorio; Canino, Baldassare; Ferrara, Filippo; Montana, M.; Raimondi, Francesco; LoPresti, Rosalia

doi:10.1053/ejvs.1999.0916

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…In previous papers regarding several venous diseases, such as deep venous thrombosis [9,19], post-thrombotic syndrome [7,8] and leg venous ulcers [20], we demonstrated, especially after in vitro activation with 4-phorbol 12-myristate 13-acetate (PMA) and with N-formyl-methionyl-leucyl-phenilalanine (fMLP), an evident alteration of the two parameters that reflect the rheology of the polymorphonuclear cells: the membrane fluidity, examined employing the fluorescent probe TMA-DPH, and the cytosolic calcium concentration, explored using the fluorescent probe Fura-2AM. In subjects with deep venous thrombosis [9] and leg venous ulcers [20] we also found an altered profile of the polymorphonuclear integrins (CD11a, CD11b, CD11c and CD18) at baseline and after activation with PMA and fMLP.…”

Section: Discussionmentioning

confidence: 99%

Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers

Caimi¹,

Ferrara²,

Montana

et al. 2015

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Venous leg ulcers are common in subjects with chronic venous insufficiency. The increased intraluminal pressure causes alteration of the skin microcirculation, leukocyte activation and release of proteolytic enzymes leading to ulceration. An impaired expression and activity of matrix metalloproteases (MMPs) and their tissue inhibitors (TIMPs) might influence extracellular matrix degradation and deposition in chronic venous ulcers with the failure of the healing process. Our aim was to evaluate plasma concentration of gelatinases (MMP-2 and MMP-9) and their inhibitors (TIMP-1 and TIMP-2) in subjects with venous leg ulcers before and after the compression therapy. We enrolled 36 subjects (12 men and 24 women, mean age 67.38 ± 12.7 yrs) with non-infected venous leg ulcers (CEAP C6), which underwent a color Duplex scan examination of the veins and arteries of the inferior limbs and were treated with a multi-layer bandaging system. The ulcer healing was obtained in 23 subjects only (9 men and 14 women). We evaluated, on fasting venous blood, the plasma levels of MMP-2, MMP-9, TIMP-1 and TIMP-2 using ELISA kit, before and after the treatment. We observed a significant increase in plasma concentration of gelatinases and their inhibitors and in MMP-2/TIMP-2 ratio in subjects with leg ulcers in comparison with normal controls. In subjects with healed ulcers we found a decrease in MMP-9 and TIMP-1 levels and in MMP-2/TIMP-2 ratio compared to the baseline values, although higher levels of all the examined parameters in comparison with normal controls. In conclusion, plasma MMPs profile is impaired in subjects with venous leg ulcers and it improves after the healing, persisting anyway altered in respect to healthy controls.

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Section: Discussionmentioning

confidence: 99%

Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers

Caimi¹,

Ferrara²,

Montana

et al. 2015

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“…We previously examined, in DVT subjects, the behavior of some parameters reflecting the func-tional activity of the PMNs (membrane fluidity and cytosolic Ca 2+ content), before and after in vitro activation with 4-phorbol 12-myristate 13acetate (PMA) and N-formyl-methionyl-leucylphenylalanine (fMLP), demonstrating a systemic PMN functional abnormality (13). To our knowledge, there are few studies concerning the PMN integrin profile in venous diseases (14).…”

mentioning

confidence: 99%

Polymorphonuclear Leukocyte Integrinsin Deep Venous Thrombosis

Caimi

Canino

Ferrara

et al. 2005

Clin Appl Thromb Hemost

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The polymorphonuclear leukocytes (PMN) have a role in the pathophysiology of deep venous thrombosis (DVT). We examined the phenotypical expression of PMN beta(2M)-integrins (CD ll a, CDll b, CD 11c) in a group of 19 subjects with leg DVT. PMN cells were incubated with fluorescent monoclonal antibodies against CD11a, CD11b, CD11c, and the evaluation was made by flow cytofluorimetry. The same integrins were determined after in vitro activation with 4-phorbol 12-myristate 13-acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). In DVT subjects, at baseline, the phenotypical expression of CD11b was decreased and that of CD11c increased when compared with normal controls. In normal subjects PMN activation with PMA and fMLP led to a constant increase of all PMN adhesion molecules, while in DVT subjects the CDl l a did not show any change. These data might have therapeutical applications, especially with the aim of preventing post-thrombotic deterioration of vein function.

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“…In diabetic disease, the behavior of the PMN membrane fluidity and cytosolic Ca 2+ concentration seems to be controversial [27,28,29,30], although the examination of these parameters suggests that they are especially dependent on the extent of the record of cases and on the glicometabolic pattern. Regarding this last aspect, we know in fact that the PMN dysfunction results significantly correlated to the PMN metabolic profile that characterizes diabetes mellitus.…”

Section: Chronic Clinical Conditionsmentioning

confidence: 99%

Fluidity and cytosolic Ca2+ concentration of circulating polymorphonuclear leukocytes at baseline in some chronic and acute clinical conditions: review of our survey

Caimi¹,

Canino²,

Presti³

et al. 2016

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Abstract. Objective: In this mini-review we describe the behavior of polymorphonuclear leukocyte (PMN) membrane fluidity and of PMN cytosolic Ca 2+ concentration in some chronic and acute clinical conditions. Methods: PMN membrane fluidity was evaluated employing the fluorescent probe Fura-2AM, and PMN cytosolic Ca 2+ concentration was evaluated using the fluorescent probe TMA-DPH. Results: From the determination of these two parameters investigated on resting PMNs, an almost constant increase in PMN cytosolic Ca 2+ concentration in chronic clinical conditions, such as vascular atherosclerotic disease with and without diabetes mellitus, essential hypertension, chronic kidney disease, and diabetes mellitus of both types, and a decrease in PMN membrane fluidity in acute clinical conditions, such as juvenile acute myocardial infarction and acute ischemic stroke, are evident. Conclusion: The possible reasons for this different behavior are analyzed on the basis of pathophysiological considerations.

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Leukocyte Rheology Before and After Chemotactic Activation in some Venous Diseases

Abstract: there is a functional alteration of leukocytes in these patients whose mechanisms are not yet clear.

Cited by 5 publications

References 22 publications

Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers

Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers

Polymorphonuclear Leukocyte Integrinsin Deep Venous Thrombosis

Fluidity and cytosolic Ca2+ concentration of circulating polymorphonuclear leukocytes at baseline in some chronic and acute clinical conditions: review of our survey

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