Leukocytic Invasion of the Ovine Cervix at Parturition

Owiny, James; Gilbert, R.O.; Wahl, Corinne; Nathanielsz, Peter W.

doi:10.1177/107155769500200402

Cited by 13 publications

(11 citation statements)

References 23 publications

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“…ROS augment around lambing is likely to be due to the massive leucocyte cervical infiltration, as previously described by Owiny et al. () and to the increase in the enzymatic and metabolic activities characterizing lambing. In this study, ROS trend reflects oestrogen fluctuations typical of lambing, which increase approximately 48 hours before lambing and sharply decrease 24 hours after it (Osman et al.…”

Section: Ros and Pregnancysupporting

confidence: 59%

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Oxidative Stress During Pregnancy In The Sheep

Mutinati¹,

Piccinno²,

Roncetti³

et al. 2013

Reprod Domestic Animals

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During physiological pregnancy, all tissues and, mostly, placenta and foetus require high amounts of oxygen. Reactive oxygen species (ROS), generated both by mother and foetus, are implicated in foetal growth because they promote replication, differentiation and maturation of cells and organs. Nevertheless, ROS excess, if not properly counterbalanced, may lead to an alteration in cell constituents, with harmful effects both on mother and foetus.ROS exert a biphasic effect because adequate ROS concentration is essential for embryo development, implant, foetal defence against uterine infections, steroidogenesis, pregnancy maintainance and partum. On the other hand, an uncontrolled ROS generation, beyond physiological antioxidant defences, may lead to embryo resorption, placental degeneration with subsequent alteration in maternal-foetal exchanges, delay in foetal growth, pregnancy interruption, stillbirths. This review investigates the mechanisms underlying ROS generation and effects, throughout physiological and pathological pregnancy in sheep, with a look to antioxidants and their importance in such a critical phase of the reproductive cycle of the sheep.

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Section: Ros and Pregnancysupporting

confidence: 59%

“…This leucocyte migration is triggered by an increase in cytokine generation, among which interleukin 1, 6, 8 (IL‐1, IL‐6, IL‐8), TNF α and other chemotactic factors, in the uterus and cervix (Owiny et al. ; Golightly et al. ).…”

Section: Ros and Pregnancymentioning

confidence: 99%

Oxidative Stress During Pregnancy In The Sheep

Mutinati¹,

Piccinno²,

Roncetti³

et al. 2013

Reprod Domestic Animals

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show abstract

“…(2008) have established the physiological range of ROS in periparturient sheep, observing an augment of serum ROS concentrations as a consequence and a cause of the physiological inflammation, at lambing. In fact, a strong leucocyte infiltration has been reported to occur in the cervix of the ewe (Owiny et al. 1995).…”

Section: Ros and Parturitionmentioning

confidence: 99%

Roles of Reactive Oxygen Species in Female Reproduction

Rizzo

Roscino

Binetti

et al. 2011

Reprod Domestic Animals

151

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Reactive oxygen species (ROS) are highly reactive oxidizing agents. Cells, under aerobic conditions, have a defence system against ROS, and in normal circumstances, there is an appropriate balance between pro-oxidants and antioxidants. When an overproduction of ROS develops or the body fails to eliminate ROS in excess, oxidative stress arises, during which ROS accumulate and damage cells and tissues. Besides their noxious effects, accumulating data have shown that controlled and adequate ROS concentrations exert physiologic functions. Different studies have confirmed the presence of ROS and the transcripts of the various antioxidant enzymes in the female reproductive tract. When ROS production overwhelms antioxidant defences, oxidative stress occurs, which may deeply threaten the anatomical and functional integrity of the genital tract. This review addresses the main physiological and pathological roles exerted by ROS and their scavenging systems in several processes involved in the main physiological functions of the female reproductive tract of both women and domestic animals. Particularly, the involvement of the oxidant system in several reproductive processes is investigated, such as follicular development, ovarian steroidogenesis, ovulation, corpus luteum formation and function, luteolysis, germ cell function, maintenance of pregnancy and beginning of parturition.

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“…Several studies have documented that parturition is characterized by an influx of inflammatory cells into the reproductive tract in both humans and animal species, including mice and sheep [8][9][10][11] . Chemokines and cytokines are capable of attracting a wide variety of cells, including macrophages, neutrophils, lymphocytes and mast cells to the site of inflammation and, thus, may participate in the process of labor 12 .…”

Section: Introductionmentioning

confidence: 99%

Amniotic fluid levels of immunoreactive monocyte chemotactic protein-1 increase during term parturition

Esplin

Romero

Chaiworapongsa

et al. 2003

The Journal of Maternal-Fetal & Neonatal Medicine

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Objective: Parturition is characterized by an influx of inflammatory cells into gestational tissues, a phenomenon conducive to increased myometrial contractility, cervical ripening and decidual/ membrane activation. Monocyte chemotactic protein-1 (MCP-1), a potent chemoattractant and activator of monocytes/macrophages, is expressed in gestational tissues and, thus, may participate in the final common pathway of labor. This study was undertaken to determine whether the amniotic fluid concentrations of immunoreactive MCP-1 are altered with gestational age or spontaneous labor at term with and without prelabor rupture of the gestational membranes. We also sought to identify intrapartum differences in the concentrations of immunoreactive MCP-1 between the upper and lower amniotic fluid compartments. Methods: A cross-sectional study was conducted to assess the concentrations of immunoreactive MCP-1 in amniotic fluid. Amniotic fluid samples were obtained from 225 women as follows:(1) women undergoing mid-trimester (14-18 weeks of gestation) amniocentesis for genetic indications, whose pregnancy outcome was normal (n = 84); (2) women in labor (n = 52) and not in labor (n = 31) at term, with intact gestational membranes; (3) women with rupture of the gestational membranes in labor (n = 18) and not in labor (n = 26), at term; and (4) women in labor at term for whom paired amniotic fluid samples were obtained through transvaginal and transabdominal amniocenteses (n = 14). Immunoreactive MCP-1 was assessed with a specific and sensitive immunoassay that had been validated for amniotic fluid. Non-parametric statistics were used for analysis. Results: Immunoreactive MCP-1 was detected in all amniotic fluid samples. Spontaneous human parturition was associated with a significant increase in the amniotic fluid concentrations of immunoreactive MCP-1 (not in labor: median 595 pg/ml, range 183-3579 pg/ml vs. in labor: median 862 pg/ml, range 183-9609 pg/ml; p = 0.01). The median amniotic fluid concentrations of immunoreactive MCP-1 were significantly higher in the lower amniotic fluid compartment than in the upper amniotic fluid compartment (lower compartment: median 2913 pg/ml, range 1360-17 080 pg/ml vs. upper compartment: median 1603 pg/ml, range 1070-8062 pg/ml; p = 0.004.). Spontaneous rupture of the gestational membranes at term was not associated with a significant change in the amniotic fluid concentrations of immunoreactive MCP-1. Conclusions: Immunoreactive MCP-1 is a physiological constituent of the amniotic fluid. The amniotic fluid levels of immunoreactive MCP-1 increase during spontaneous labor at term. A topographic difference in the concentration of immunoreactive MCP-1 was observed in the amniotic cavity, with higher concentrations being noted in the lower amniotic fluid compartment, as compared with the upper amniotic fluid compartment. These findings support the hypothesis that MCP-1 may play a role in the final common pathway of spontaneous labor.

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Leukocytic Invasion of the Ovine Cervix at Parturition

Cited by 13 publications

References 23 publications

Oxidative Stress During Pregnancy In The Sheep

Oxidative Stress During Pregnancy In The Sheep

Roles of Reactive Oxygen Species in Female Reproduction

Amniotic fluid levels of immunoreactive monocyte chemotactic protein-1 increase during term parturition

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