Leukoencephalopathy Associated with Parvovirus Infection in Cretan Hound Puppies

Schaudien, Dirk; Polizopoulou, Z. S.; Koutinas, A. F.; Schwab, Stephanie; Porombka, Doris; Baumgärtner, Wolfgang; Herden, Christiane

doi:10.1128/jcm.01582-09

Cited by 31 publications

(38 citation statements)

References 53 publications

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“…It is noteworthy that five of twelve samples belonging to this cluster were from brains of puppies with clinical signs of parvovirosis. As already reported, CPV can be detected in the brain in experimental and natural infections [ 9 , 30 ]. The 12 CPV-2b strains account for about 44% of those examined, and they formed a separate cluster in the phylogenetic tree, indicating regional genetic variation of CPV-2 strains [ 17 ].…”

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confidence: 58%

First molecular characterization of canine parvovirus strains in Sardinia, Italy

et al. 2017

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Canine parvovirus type 2 (CPV-2) is responsible of acute hemorrhagic gastroenteritis in young dogs. CPV-2 emerged in 1978 in the USA, but new antigenic types, CPV-2a, 2b and 2c, have completely replaced the original type. In this study, we analyzed 81 animals collected in Sardinia, Italy. The VP2 sequence analysis of 27 positive samples showed that all antigenic CPV-2 types are circulating. CPV-2b seems to be the most widespread variant, followed by CPV-2a. Furthermore, 12 CPV-2b strains displayed further amino acid substitutions and formed a separate cluster in a phylogenetic tree, indicating regional genetic variation.

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confidence: 58%

First molecular characterization of canine parvovirus strains in Sardinia, Italy

et al. 2017

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“…Most of the viruses belonging to the Parvoviridae family replicate in mitotically active cells such as intestinal crypt epithelial cells; however, recent studies demonstrated the presence of parvoviruses in typically nonmitotic cells such as neurons. 11,34,36 Furthermore, the pathogenesis of bocaviruses with respect to tissue tropism and replication is not well documented. In this way, the CBoV-2 infection in these puppies resembled that of CBoV-1, which shows viral inclusion bodies in enterocytes.…”

Section: Discussionmentioning

confidence: 99%

Canine Bocavirus Type 2 Infection Associated With Intestinal Lesions

Piewbang

Puff

et al. 2018

Vet Pathol

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Bocaviruses are small nonenveloped DNA viruses belonging to the Bocaparvovirus genus of the Parvoviridae family and have been linked to both respiratory and enteric disease in humans and animals. To date, 3 bocaviruses, canine bocaviruses 1 to 3 (CBoV-1-3), have been shown to affect dogs with different disease manifestations reported for infected animals. We used next-generation sequencing to identify a novel strain of canine CBoV-2 (CBoV TH-2016) in a litter of puppies that died in Thailand from acute dyspnea and hemoptysis, for which no causal pathogen could be identified in routine assays. Analysis of the complete coding sequences of CBoV TH-2016 showed that this virus was most closely related to a strain previously identified in South Korea (isolate 14D193), with evidence of genetic recombination in the VP2 gene with related strains from South Korea and Hong Kong. Use of quantitative polymerase chain reaction showed the presence of CBoV TH-2016 in several tissues, suggesting hematogenous virus spread, while only intestinal tissue was found to be positive by in situ hybridization and electron microscopy. Histologic small intestinal lesions associated with CBoV TH-2016 infection were eosinophilic intranuclear inclusion bodies within villous enterocytes without villous atrophy or fusion, similar to those previously considered pathognomonic for CBoV-1 infection. Therefore, this study provides novel insights in the pathogenicity of canine bocavirus infections and suggests that a novel recombinant CBoV-2 may result in atypical findings of CBoV infection. Although the specific cause of death of these puppies remained undetermined, a contributory role of enteric CBoV TH-2016 infection is possible.

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“…Liquid phase immune-electron microscopy was performed on a suspension of intestinal sample material using a high titred polyclonal serum against canine parvovirus (CPV). Immunohistochemistry to detect CPV and canine distemper virus (CDV) antigen was performed on sections of small intestine, spleen, lymph node and bone marrow using a monoclonal mouse anti-CPV1-2A1 antibody (Custom Monoclonal Antibodies International, Sacramento, CA) and a monoclonal mouse anti-CDV nucleoprotein antibody (D110, kindly provided by A. Zurbriggen, Berne, Switzerland) as previously described (Schaudien et al, 2010;von Rü den et al, 2012). In addition, to investigate involvement of bacterial agents, a sample of small intestine of one puppy was incubated on standard agar under aerobic and anaerobic conditions (Department of Microbiology, University of Veterinary Medicine Hannover, Germany).…”

Section: Routine Virological Laboratory Diagnosticmentioning

confidence: 99%

Novel canine bocavirus strain associated with severe enteritis in a dog litter

Bodewes

Lapp²,

Hahn³

et al. 2014

Veterinary Microbiology

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Bocaviruses are small non-enveloped viruses with a linear ssDNA genome, that belong to the genus Bocaparvovirus of the subfamiliy Parvovirinae. Bocavirus infections are associated with a wide spectrum of disease in humans and various mammalian species. Here we describe a fatal enteritis associated with infection with a novel strain of canine bocavirus 2 (CaBoV-2), that occurred in a litter of German wirehaired pointers. Necropsy performed on three puppies revealed an enteritis reminiscent of canine parvovirus associated enteritis, accompanied with signs of lymphocytolytic disease in bone marrow, spleen, lymph nodes and thymus. While other major causes of enteritis of young dogs, including canine parvovirus, were excluded, by random PCR in combination with next-generation sequencing, a novel CaBoV-2 strain was detected. Phylogenetic analysis of the genome of this novel canine bocavirus strain indicated that this virus was indeed most closely related to group 2 canine bocaviruses. Infection with canine bocavirus was confirmed by in situ hybridization, which revealed the presence of CaBoV-2 nucleic acid in the intestinal tract and lymphoid tissues of the dogs. In a small-scale retrospective analysis concerning the role of CaBoV-2 no additional cases were identified. The findings of this study provide novel insights into the pathogenicity of canine bocaviruses.

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Leukoencephalopathy Associated with Parvovirus Infection in Cretan Hound Puppies

Cited by 31 publications

References 53 publications

First molecular characterization of canine parvovirus strains in Sardinia, Italy

First molecular characterization of canine parvovirus strains in Sardinia, Italy

Canine Bocavirus Type 2 Infection Associated With Intestinal Lesions

Novel canine bocavirus strain associated with severe enteritis in a dog litter

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