Leukotriene B4 receptor BLT-mediated phosphorylation of NF-κB and CREB is involved in IL-8 production in human mast cells induced by Trichomonas vaginalis-derived secretory products

SNAP23-Dependent Surface Translocation of Leukotriene B 4 (LTB 4 ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secreted LTB 4

“…Therefore, we suggest that T. vaginalis-secreted LTB 4 plays a crucial role in exocytotic degranulation in HMC-1 cells. It has been reported that the TvSP induce IL-8 production in mast cells via BLT1 (21). We also found that pretreatment of BLT1 antagonist or BLT1 siRNA inhibited surface CD63 upregulation and histamine release in HMC-1 cells induced by TvSP (Fig.…”

“…In a previous study, we found that T. vaginalis itself secretes LTB 4 (21). This study demonstrated for the first time that SNAP23-dependent surface translocation of BLT1 is important for NOX2-dependent exocytotic degranulation in human mast cells induced by T. vaginalis-secreted LTB 4 .…”

“…These results suggest that T. vaginalis-derived leukotrienes play an important role in the mast cell responses. (21). BLT1 is a kind of G protein-coupled receptor (GPCR) that has a high affinity for lipid mediator LTB 4 in mast cells.…”

“…Upon activation by stimulation by chemotactic factors or chemokines, mast cells arrive in the inflamed tissues. These mast cells are further activated by the proinflammatory microenvironment, which ultimately leads to diverse cellular responses, such as adhesion to extracellular matrix proteins, neutrophils, or T cells, degranulation, and production of cytokines, chemokines, and/or cysteinyl leukotrienes (17)(18)(19)(20)(21)(22). Among these responses, degranulation plays a direct role in eliciting mast cell-mediated tissue inflammatory responses (23).…”

“…TvSP induced upregulation of CD63 expression and reactive oxygen species (ROS) generation in HMC-1 cells via activation of mitogen-activated protein kinases (MAPKs) (35). T. vaginalis-derived LTB 4 can induce HMC-1 cells to release IL-8 via LTB 4 receptor (BLT)-dependent activation of NF-B and CREB (21). Similarly, in neutrophils, NF-B and CREB were involved in IL-8 production induced by TvSP in BLT-dependent signaling (36,37).…”