Leukotriene C4 production by normal-density and low-density eosinophils of atopic individuals and other patients with eosinophilia1

Kauffman, Hf; Vanderbelt, B; Demonchy, Jgr; Boelens, Hendrik M.; Koeter, Gh; Devries, K

doi:10.1016/s0091-6749(87)80157-4

Cited by 51 publications

(25 citation statements)

References 22 publications

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“…Stimulation of human and animal eosinophils with various soluble and nonsoluble agents leads to the formation of various arachidonic acid-derived lipids, including LTC 4 [63,69,[115][116][117], PGE [118,119], prostaglandin F 1 (PGF 1 ) and TxA 2 [64,[119][120][121][122][123], PGD 2 and prostaglandin F 2α (PGF 2α ) [64,123]. In addition, eosinophils have been shown to synthesize large amounts of PAF [117,[124][125][126].…”

Section: Oxygen Metabolitesmentioning

confidence: 99%

“…For instance, hypodense cells obtained from asthmatic subjects produced a lower amount of reactive oxygen metabolites in response to zymosan and phorbol myristate acetate (PMA), as assessed by nitro blue tetrazolium (NBT) reduction and chemiluminescence [4,147]. In addition, low-density eosinophils obtained from asthmatic subjects produced only half the amount of LTC 4 compared with normodense cells [115]. Furthermore, BAL, eosinophils from asthmatic subjects obtained 18 h post-allergen challenge showed a reduced capacity to generate prostanoids compared to blood eosinophils [163].…”

Section: Functional Heterogeneitymentioning

confidence: 99%

See 1 more Smart Citation

Pulmonary immune cells in health and disease: the eosinophil leucocyte (Part I)

Kroegel¹,

Virchow²,

Luttmann³

et al. 1994

Eur Respir J

129

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P Pu ul lm mo on na ar ry y i im mm mu un ne e c ce el ll ls s i in n h he ea al lt th h a an nd d d di is se ea as se e: : t th he e e eo os si in no op ph hi il l l le eu uc co oc cy yt te e ( (P Pa ar rt t I I) ) ABSTRACT: Increasing evidence has accumulated to suggest that eosinophils play a key role in the pathogenesis of asthma and other pulmonary diseases by damaging infiltrated bronchial tissue and lung parenchyma. The first part of this review on eosinophils describes the cellular characteristics and properties of the cell, which help in understanding its role in disease. The article focuses on origin, maturation and differentiation of the eosinophil, its morphological and phenotypical properties, as well as its preformed and newly generated mediators of inflammation. The cause and putative significance of eosinophil heterogeneity in respect to function and density will also be discussed. In addition, the naturally occurring mediators through which eosinophils are activated and communicate with other inflammatory cells are outlined.The first part closes with new aspects of eosinophil recruitment from the circulation into perivascular tissue, including nonselective and putative selective adhesion mechanisms and chemotaxis.

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Section: Oxygen Metabolitesmentioning

confidence: 99%

Section: Functional Heterogeneitymentioning

confidence: 99%

Pulmonary immune cells in health and disease: the eosinophil leucocyte (Part I)

Kroegel¹,

Virchow²,

Luttmann³

et al. 1994

Eur Respir J

129

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“…Leukotrienes, specially LTB4, have been shown to be potent eosinophil chemoattractants both in vivo (Faccioli et al, 1991) and in vitro (Czarnetzki & Mertensmeier, 1985;Sehmi et al, 1991). Interestingly, human eosinophils also release LTC4 which may contribute to bronchoconstriction in airway disease (Kauffman et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Effect of a 5‐lipoxygenase inhibitor, ZM 230487, on cutaneous allergic inflammation in the guinea‐pig

Teixeira

Hellewell

1994

British J Pharmacology

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Leukotrienes have potent biological effects in vitro and in vivo and are found in tissue and in biological fluids in various pathological conditions including allergic diseases. Leukotriene B4 (LTB4) is a potent stimulus for eosinophil accumulation and activation and there is much interest in determining its importance in mediating the accumulation of eosinophils at sites of allergic inflammation in vivo. In this study, we investigated the effects of a potent 5‐lipoxygenase inhibitor, ZM 230487, on the accumulation of eosinophils and on local oedema formation in cutaneous inflammation in the guinea‐pig. The i.d. injection of increasing concentrations of arachidonic acid (AA) led to a dose‐dependent accumulation of 111In‐eosinophils but oedema formation was only significant at the top dose of AA tested (3 × 10−8mol per site). Co‐injection of ZM 230487 with AA inhibited 111In‐eosinophil accumulation up to 99% but the small oedema response to AA was only partially inhibited. AA‐induced oedema formation was only effectively inhibited when a combination of a PAF antagonist, an antihistamine and ZM 230487 was used. Local administration of the cyclo‐oxygenase inhibitor, ibuprofen, partially inhibited AA‐induced oedema formation suggesting that vasodilator prostaglandins may be released following i.d. injection of AA. AA‐induced 111In‐eosinophil accumulation was also partially inhibited by ibuprofen. PAF‐induced 111In‐eosinophil accumulation was partially suppressed by local administration of ZM 230487. In contrast, LTB4‐induced 111In‐eosinophil accumulation was enhanced by ZM 230487. These data suggest that locally‐released leukotrienes may modulate mediator‐induced eosinophil accumulation. ZM 230487 had no effect on PAF‐or LTB4‐induced oedema formation. ZM 230487 significantly inhibited the accumulation of 111In‐eosinophils, but did not affect local oedema formation, in a passive cutaneous anaphylaxis (PCA) reaction. However, the PAF antagonist WEB 2086 either alone or in combination with ZM 230487 had no effect on 111In‐eosinophil accumulation or oedema formation in the PCA reaction. In conclusion, it appears that a product of 5‐lipoxygenase, probably LTB4, is important for the accumulation of 111In‐eosinophils, but not local oedema formation, in the PCA reaction in guinea‐pig skin. These data support a major role for LTB4 in allergic inflammation in the guinea‐pig and make this animal (and the PCA model) suitable for studying the effects of inhibitors of leukotriene synthesis or action in vivo.

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“…There is strong evidence that the eosino phil plays an important role in the late broncho-ob structive reaction [2][3][4], especially by its ability to syn thesize leukotriene C4 (LTC4) [5][6][7]. LTC4 is a strong broncho-obstructive compound which is a compo nent of the slow reaction substance of anaphylaxis [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…LTC4 is a strong broncho-obstructive compound which is a compo nent of the slow reaction substance of anaphylaxis [8,9]. The eosinophilic cell population in asthmatic patients differs in density compared to nonatopic healthy subjects [6,10,11]. Conflicting results have been published in the literature.…”

Section: Introductionmentioning

confidence: 99%

Cellular Communication in Leukotriene C<sub>4</sub> Production between Eosinophils and Neutrophils

Kloprogge

Deleeuw²,

Demonchy³

et al. 1989

Int Arch Allergy Immunol

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Eosinophilic granulocytes as well as neutrophilic granulocytes produced leukotriene C₄ (LTC₄) on stimulation with 1 μM A23187 (Ca²⁺ ionophore). In healthy volunteers, the LTC₄ production in eosinophils was about 3 times the production in neutrophils. Within 15 min >90% of the LTC₄ was released into the supernatant. Stimulation of eosinophils and neutrophils together resulted in a synergistic increase in LTC₄ production of 306 ± 40%. LTC₄ synthesis by hypodense eosinophils was also enhanced if stimulated in the presence of neutrophils. These findings suggest a communication between eosinophils and neutrophils, which may play a role in bronchial asthma.

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Leukotriene C4 production by normal-density and low-density eosinophils of atopic individuals and other patients with eosinophilia1

Cited by 51 publications

References 22 publications

Pulmonary immune cells in health and disease: the eosinophil leucocyte (Part I)

Pulmonary immune cells in health and disease: the eosinophil leucocyte (Part I)

Effect of a 5‐lipoxygenase inhibitor, ZM 230487, on cutaneous allergic inflammation in the guinea‐pig

Cellular Communication in Leukotriene C<sub>4</sub> Production between Eosinophils and Neutrophils

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