Leukotriene transformation by guinea-pig lungs

Sirois, Pierré; Brousseau, Yvon

doi:10.1016/s0262-1746(83)80004-3

Cited by 15 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The LT-LS could therefore be either LTD4 or LTE4, which are both capable of contracting the assay tissues, although their potency is different (Lewis et al, 1981). Since LTC4 is rapidly metabolized to LTD4 by guinea-pig lung homogenates (Sirois & Brousseau, 1983) and LTD4 is the major component of guinea-pig slow-reacting substance of anaphylaxis (SRS-A; Morris et al, 1982), in our experiments the LT-LS is most probably LTD4. It is relevant to note that synthesis of SRS-A (probably a mixture of LTC4 and LTD4) by lung in response to ionophore stimulation has already been demonstrated (Piper & Seale, 1979).…”

Section: Discussionmentioning

confidence: 60%

“…The LT-LS could therefore be either LTD4 or LTE4, which are both capable of contracting the assay tissues, although their potency is different (Lewis et al, 1981). Since LTC4 is rapidly metabolized to LTD4 by guinea-pig lung homogenates (Sirois & Brousseau, 1983) (Piper & Seale, 1979). It is interesting that the other two stimuli (AA and bradykinin) did not cause the formation of a leukotriene-like substance, since in the lung parenchymal strip of the guinea-pig, incubation with AA leads to the formation of LTC4 and LTD4 (Burka & Saad, 1984).…”

Section: Ratios Of Eicosanoids Formedmentioning

confidence: 99%

See 1 more Smart Citation

Differential release of eicosanoids by bradykinin, arachidonic acid and calcium ionophore A23187 in guinea‐pig isolated perfused lung

Bakhle

Moncada

Nucci³

et al. 1985

British J Pharmacology

View full text Add to dashboard Cite

IThe effects of infusions of bradykinin (0.2 fLM), calcium ionophore A23 187 (0.5 LM) and arachidonic acid (13 LM) on the release of eicosanoids from the guinea-pig isolated perfused lung were investigated using radioimmunoassay for thromboxane B2 (TXB2), 6-oxo-prostaglandin Flm (6-oxo-PGF,.), PGE2, leukotriene B4 (LTB4) and LTC4 and bioassay using the superfusion cascade. 2 Bradykinin released more 6-oxo-PGF,, than TXB2, whereas arachidonic acid and ionophore released more TXB2 than 6-oxo-PGF,,.3 The time course of eicosanoid release varied with the stimulus: bradykinin and arachidonic acid produced an immediate release, whereas the ionophore showed a slower onset of release. 4 Although the amounts of LTB4 and LTC4 released by the ionophore were very low according to radioimmunoassays, there was evidence from the bioassay of release of a leukotriene-like substance, thought to be LTD4. 5 The leukotriene antagonist FPL 55712 lacks specificity in the guinea-pig trachea; at the concentration used (2 tiM) it antagonized contractions of the tracheal strip to PGE2 as well as to LTC4. 6 Our results show that in the guinea-pig perfused lung the metabolism of exogenous arachidonic acid is both qualitatively and quantitatively different from the metabolism of endogenous arachidonic acid; furthermore, the profile of eicosanoid production is stimulus-dependent.

show abstract

Section: Discussionmentioning

confidence: 60%

“…The LT-LS could therefore be either LTD4 or LTE4, which are both capable of contracting the assay tissues, although their potency is different (Lewis et al, 1981). Since LTC4 is rapidly metabolized to LTD4 by guinea-pig lung homogenates (Sirois & Brousseau, 1983) (Piper & Seale, 1979). It is interesting that the other two stimuli (AA and bradykinin) did not cause the formation of a leukotriene-like substance, since in the lung parenchymal strip of the guinea-pig, incubation with AA leads to the formation of LTC4 and LTD4 (Burka & Saad, 1984).…”

Section: Ratios Of Eicosanoids Formedmentioning

confidence: 99%

Differential release of eicosanoids by bradykinin, arachidonic acid and calcium ionophore A23187 in guinea‐pig isolated perfused lung

Bakhle

Moncada

Nucci³

et al. 1985

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…Previous reports [22,23] have shown that the activity of dipeptidase in normal plasma is higher than that of y-glutamyl transpeptidase. Our data both in tlivo and in vitro, corroborate this finding and indicate a decreased activity of both y-glutamyl transpeptidase and dipeptidase in HbSS disease.…”

Section: Discussionmentioning

confidence: 82%

Plasma and urinary leukotrienes in sickle cell disease: possible role in the inflammatory process

Ibe

Kurantsin‐Mills

Raj

et al. 1994

Eur J Clin Investigation

View full text Add to dashboard Cite

Sickle cell (HbSS) disease is associated with rheological and inflammatory stresses within the microcirculation. In order to determine the role of leukotrienes in the inflammatory processes in HbSS patients, we analysed plasma and urine levels of leukotrienes (LT); LTB4, LTC4, LTD4, and LTE4 as indicators of their in vivo metabolism. Plasma and urine level samples of 15 HbSS patients in steady-state and age-matched healthy, homozygous (HbAA) controls were extracted for leukotrienes and quantitated by HPLC. Control plasma level of leukotrienes (mean +/- SEM, ng ml-1) were: LTB4, 8.95 +/- 0.26; LTC4, 7.24 +/- 0.21; LTD4, 11.42 +/- 0.40; and LTE4, 14.51 +/- 0.50. Corresponding values for HbSS patients were: LTB4, 6.15 +/- 0.42; LTC4, 13.61 +/- 1.45; LTD4, 6.44 +/- 0.51 and LTE4, 4.97 +/- 0.37. The differences were significant at P < 0.05. Urine levels (mean +/- SEM, ng mmol-1 creatinine), for controls were: LTB4, 10.60 +/- 0.35; LTC4, 360.0 +/- 9.82. Values for HbSS urine were: LTB4, 27.50 +/- 3.33; LTC4, 356.0 +/- 17.87; LTD4, 69.90 +/- 14.51. LTD4 was not detected in control urine. These results suggest that sickle cell patients may exhibit impaired ability to catabolize LTC4 in plasma during steady state conditions. This altered metabolism may contribute to the persistent stress of the microcirculation, and is probably related to the abnormal microvascular rheology of sickle blood cells.

show abstract

Synthesis of Prostaglandins and Leukotrienes Effects of Ethanol

Murphy

Westcott

1985

Recent Developments in Alcoholism

View full text Add to dashboard Cite

Leukotriene transformation by guinea-pig lungs

Cited by 15 publications

References 20 publications

Differential release of eicosanoids by bradykinin, arachidonic acid and calcium ionophore A23187 in guinea‐pig isolated perfused lung

Differential release of eicosanoids by bradykinin, arachidonic acid and calcium ionophore A23187 in guinea‐pig isolated perfused lung

Plasma and urinary leukotrienes in sickle cell disease: possible role in the inflammatory process

Synthesis of Prostaglandins and Leukotrienes Effects of Ethanol

Contact Info

Product

Resources

About