Levansucrase production by Zymomonas mobilis : Optimization of process parameters and fructooligosaccharide production

Abstract

This article investigates the production and optimization of levansucrase using synthetic medium by Zymomonas mobilis NRRL B‐14023. Response surface methodology was used to investigate the effects of three different fermentation parameters on levansucrase enzyme production. Maximum levansucrase activity of 13.3 µmol glucose/min was obtained at the optimum levels of process variables (pH 4.90, initial sucrose concentration 159.01 g/L and fermentation temperature 30.3°C). Response surface methodology was found to be useful in optimizing and determining the interactions among process variables. The purity of levansucrase used in enzyme assays were analyzed by SDS‐PAGE and a dominant protein band of size around 47 kDa was obtained. The sequence of the produced enzyme was determined by peptide mass fingerprinting. The presence of kestose in the reaction mixture showed that Z. mobilis levansucrase can produce fructooligosaccharides. This study fulfills the lack of mathematical and statistical approaches in optimizing the levansucrase production by Z. mobilis. Practical applications Levansucrases are responsible for the production of valuable fructans; fructooligosaccharides (FOS) and levan. Fructooligosaccharides are sugar polymers three times less sweet than sucrose and naturally present in some foods. FOS have important promising use in the food industry due to their properties such as solubility in water, stability during storage, low calorie value, and prebiotic effects. In this research, the production of microbial levansucrase was optimized for potential industrial applications by response surface methodology. Future studies will focus on conversion of intrinsic sucrose of foods to FOS using levansucrase.

The Complex GH32 Enzyme Orchestra from Priestia megaterium Holds the Key to Better Discriminate Sucrose-6-phosphate Hydrolases from Other β-Fructofuranosidases in Bacteria

“…Except for the levansucrase of Z. mobilis, most of the enzymes known to produce 6-kestotriose are from yeast or fungal origin, complicating the industrial production process of 6-kestotriose on a greater scale. – Therefore, SacAP3 may be a promising alternative. In addition, future structure–function studies on SacAP3 may contribute to our understanding on which active site residues are crucial in the formation of either β-(2 → 1) or β-(2 → 6) linkages …”

Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4

“…FOS are natively produced in Z. mobilis by SacB enzyme (Bekers et al 2002;Santos-Moriano et al 2015;Erdal et al 2017;Taştan et al 2019). As an attempt to modulate the composition of FOS mixture, two different strains were constructed.…”

Zymomonas mobilis as an emerging biotechnological chassis for the production of industrially relevant compounds

“…These compounds are carbohydrates composed of fructose residues with a terminal glucose molecule residue linked by β(2 → 1) glycosidic bonds (Flores-Maltos et al 2016). As already mentioned, FOS are produced from sucrose by Z. mobilis SacB enzyme (Bekers et al 2002;Santos-Moriano et al 2015;Erdal et al 2017;Taştan et al 2019) (Fig. 2).…”