Level of Interleukin-17 in Gingival Crevicular Fluid of Patients with Chronic Periodontitis

Abstract

Aim: Interleukin-17 (IL-17) is a proinflammatory cytokine that plays an important role in inflammation and tissue destruction in periodontal disease. This study aimed to assess the level of IL-17 in gingival crevicular fluid (GCF) of patients with chronic periodontitis (CP) and healthy controls. Materials and methods:This case-control study was performed on 30 patients with CP (53% males, 47% females, mean age of 37.2 ± 5.95 years) and 30 healthy controls (53% males, 47% females, mean age of 30.63 ± 5.22 years). The GCF was collected using paper points. A paper point was inserted into the pocket and remained there for 30 seconds. It was then placed in a sterile tube containing 300 µL of phosphate buffered saline and stored at -70° C. Level of IL-17 was measured using enzyme-linked immune sorbent assay (ELISA). The level of IL-17 was compared between the two groups using independent sample t-test at 0.05 level of significance. Results:The mean GCF level of IL-17 was 53.46 pg/L in CP patients and 38.1 pg/L in healthy controls. This difference was statistically significant (p = 0.025). Conclusion:The CP patients had significantly higher GCF level of IL-17 compared to healthy controls. Clinical significance:The finding of this study highlight the role of IL-17 in the pathogenesis of periodontal disease. Within the limitations of the present study, it may be suggested that measurement of GCF level of IL-17 can serve as a bioindicator of periodontal destruction and gingival inflammation.

IL-23/IL-17 axis levels in gingival crevicular fluid of subjects with periodontal disease: a systematic review

“…The oldest study was from 2005 [ 39 ], and the most recent from 2022 [ 28 ]. Five (41.6%) studies were conducted in India [ 28 – 30 , 35 , 36 ], two (16.6%) studies in Iran [ 31 , 32 ], and other studies (8.3%) in Saudi Arabia [ 33 ], Japan [ 34 ], Egypt [ 37 ], Turkey [ 38 ], and Chile [ 39 ]. In addition, the title and journals of publication are shown (Table 2 ).…”

“…Nine (75%) studies collected GCF samples with absorbent paper strips [ 30 – 35 , 37 – 39 ] and 3 (25%) studies reported GCF collection using volumetric microcapillary pipettes [ 28 , 29 , 36 ]. Among the 12 included studies, 4 (33.3%) used ELISA kits without brand specification [ 30 , 32 – 34 ], two (16.6%) used R&D Systems ELISA kit [ 36 , 39 ], two others (16.6%) used BioSource ELISA kit [ 37 , 38 ], others (8.3%) used RayBiotech [ 28 ], Diaclone [ 29 ], Bender Med Systems [ 31 ] and eBioscience [ 35 ]. Most of the studies (75%) analyzed cytokine IL-17 levels individually [ 28 – 30 , 32 , 34 , 36 – 39 ], whereas, 2 others (17%) analyzed IL-23 levels [ 33 , 35 ] and only one (8%) analyzed the IL-23/IL-17 axis together [ 31 ] (Table 3 ).…”

Plaque Index and Gingival Index Evaluation in Subjects with Gingivitis after Two Months of Treatment with Two Different Probiotic Food Supply: A Retrospective Study

“…As regards the PI of group B, the difference between T0 and T4 is statistically significant for p <0.05, with t = -33. 16.…”

“…The latter act selectively against specific bacterial strains or species and appear capable of eliminating or interfering with the growth of other bacteria genetically "close" to the inhibiting bacterium. Furthermore, unlike viruses, are considered safe by the Food and Drug Administration for administration in humans and have therefore gained popularity in research in the field of probiotics [14]- [16].…”

“…-withstand oral conditions, -resist environmental defense mechanisms, -adhere to the saliva-coated surface, -colonize the mouth, -proliferate to inhibit oral pathogens [9][10][11][12][13][14][15][16][17].…”