Level of serum lactate dehydrogenase in oral submucous fibrosis

Abstract

<p class="abstract"><strong>Background:</strong> Oral cancer is one of the most common form of malignancies in India. In many cases it develops at the site of premalignant lesion. Of all oral premalignant conditions, oral submucous fibrosis (OSMF) is of greater concern because of its disabling nature and relative greater chances of malignant transformation. Transformation of normal tissue to premalignant lesion and further to oral cancer results in alteration in glycolytic pathway and hence the lactate dehydrogenase (LDH) levels. The aim of this study was to estimate the LDH levels in serum of subjects with OSMF and to compare them with healthy controls and to correlate the relationship between pathogenesis of OSMF and the LDH enzyme.</p><p class="abstract"><strong>Methods:</strong> It is a case control study. The study included 40 diagnosed cases of OSMF and 40 matched healthy controls. Venous blood of 3 ml was collected in both cases and controls. Serum was separated by centrifugation and LDH was estimated by using standard kits. Statistical analysis was done using student ‘t’ test. Pearson's correlation was performed to establish the relationship between study variables. </p><p class="abstract"><strong>Results:</strong> It was observed that serum LDH levels were significantly increased in cases of OSMF as compared to controls (p <0.005).</p><p><strong>Conclusions:</strong> Serum LDH was significantly increased in OSMF and can be used as a valuable biochemical marker in prognosis of OSMF.</p>

Lactate Dehydrogenase as a Biomarker in Oral Submucous Fibrosis: A Systematic Review and Meta-Analysis

“…After carefully going through the 15 complete texts, we eliminated the three articles (n=3) that did not specifically address any oral potentially malignant disorder. [1,5,10,[20][21][22][23][24][25][26][27][28][29][30][31].Finally, we included 12 articles in the systematic review as they matched the eligibility criteria (Figure 1).…”

“…In all of the examined studies, OSCC and OPMD categories had higher serum and salivary concentrations of LDH than the healthy individuals' group [5,10,20,[22][23][24][25][26][27]28,30,31]. Three eligible studies [10,23,28] evaluated higher LDH levels in the OL group when compared to the control group but lower when compared to OSCC.…”

“…Two selected studies [ 6 , 25 ] mentioned the study duration. All the studies evaluated oral submucous fibrosis out of which three studies [ 23 , 25 , 26 ] compared OSMF with OL, two studies [ 23 , 27 ] compared OSMF with OSCC and OL, three studies [ 10 , 28 , 30 ] compared OSMF with OSCC and one study [ 1 ] compared OSMF with OSCC and individuals with tobacco chewing habits.…”

“…All of the chosen studies had eligibility and exclusion criteria. Four out of 12 studies evaluated the salivary LDH level [ 10 , 22 , 25 , 31 ] and three out of 12 studies [ 26 , 27 , 30 ] evaluated the serum LDH level. All the studies collected the unstimulated whole saliva except one [ 20 ] collected the stimulated whole saliva.…”

“…Three eligible studies [ 10 , 23 , 28 ] evaluated higher LDH levels in the OL group when compared to the control group but lower when compared to OSCC. In all of the included trials, the concentration of serum and salivary LDH were higher in OSMF cases in comparison to the controls [ 5 , 10 , 12 , 20 , 22 - 28 , 30 ]. All the studies showed statistically significant “p” value.…”