Levels of 13,14-dihydro-15-keto-PGF2α in biological fluids as measured by radioimmunoassay

Levine, Lawrence; Gutierrez‐Cernosek, Rose Mary

doi:10.1016/0090-6980(73)90005-1

Cited by 32 publications

(4 citation statements)

References 13 publications

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“…(see [138][139][140][141][142][143][144][145][146][147][148] and references therein), but several assays have also been developed for prostaglandins of the E, A, or B types (149)(150)(151)(152)(153)(154)(155)(156) and references therein) and for some prostaglandin metabolites (106,(157)(158)(159)(160)(161)(162)(163)(164). Most of these have been aimed at prostaglandins of the F type, mainly PGF 2.…”

Section: Gas Chromatography-mass Spectrometrymentioning

confidence: 99%

“…A fe w papers reporting radioimmunoassays fo r 15-keto-13,14-dihydro-PGF z. have appeared (157,(159)(160)(161) and also one fo r 15-keto-13,14-dihydro-PGEz (106). Thus the assays should preferably be aimed instead at the main metabolites, t h e 15-keto-13,14-dihydro compounds.…”

Section: Gas Chromatography-mass Spectrometrymentioning

confidence: 99%

“…The main problem encountered in this type of assay was the preparation of the radio-labeled ligand. From one laboratory an average level of 4800 pg/ml (range 1,000-26,000 pg/ml) was reported (159). Others have used a different method (157,161): incubation with a swine kidney preparation in the presence of inhibitors of prostaglandin biosynthesis (112).…”

Section: Gas Chromatography-mass Spectrometrymentioning

confidence: 99%

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Prostaglandins

Samuelsson¹,

Granström²,

Green³

et al. 1975

Annu. Rev. Biochem.

567

170

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Section: Gas Chromatography-mass Spectrometrymentioning

confidence: 99%

Section: Gas Chromatography-mass Spectrometrymentioning

confidence: 99%

Section: Gas Chromatography-mass Spectrometrymentioning

confidence: 99%

See 1 more Smart Citation

Prostaglandins

Samuelsson¹,

Granström²,

Green³

et al. 1975

Annu. Rev. Biochem.

567

170

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“…The same pathway that metabolizes PGE2 and PGD2 converts also PGF2α to form through modification by 15-PGDH and 15-oxo-prostaglandin Δ13-reductase, its initial, bi ologically inactive plasma metabolite, 13,14-dihydro-15 keto PGF2α (Figure 4) [71,72] 13,14-dihydro-15 keto PGF2α accumulates in human plasma to concentrations of 0.08-2 pmol/mL [36] but prevails only briefly. Further metabolism involves β-and ω-oxidatio and leads to tetranor-PGFM [73], which is detectable in urine, but also in plasma [74].…”

Section: Metabolites Of Pgf2αmentioning

confidence: 99%

Prostanoid Metabolites as Biomarkers in Human Disease

Idborg

Pawelzik

2022

Metabolites

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Prostaglandins (PGD2, PGE2, PGF2α), prostacyclin (PGI2), and thromboxane A2 (TXA2) together form the prostanoid family of lipid mediators. As autacoids, these five primary prostanoids propagate intercellular signals and are involved in many physiological processes. Furthermore, alterations in their biosynthesis accompany a wide range of pathological conditions, which leads to substantially increased local levels during disease. Primary prostanoids are chemically instable and rapidly metabolized. Their metabolites are more stable, integrate the local production on a systemic level, and their analysis in various biological matrices yields valuable information under different pathological settings. Therefore, prostanoid metabolites may be used as diagnostic, predictive, or prognostic biomarkers in human disease. Although their potential as biomarkers is great and extensive research has identified major prostanoid metabolites that serve as target analytes in different biofluids, the number of studies that correlate prostanoid metabolite levels to disease outcome is still limited. We review the metabolism of primary prostanoids in humans, summarize the levels of prostanoid metabolites in healthy subjects, and highlight existing biomarker studies. Since analysis of prostanoid metabolites is challenging because of ongoing metabolism and limited half-lives, an emphasis of this review lies on the reliable measurement and interpretation of obtained levels.

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Immobilized Enzymes and Proteins in Urinalysis

Boguslaski¹,

Smith²

1977

Biomedical Applications of Immobilized Enzymes and Proteins

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Levels of 13,14-dihydro-15-keto-PGF2α in biological fluids as measured by radioimmunoassay

Cited by 32 publications

References 13 publications

Prostaglandins

Prostaglandins

Prostanoid Metabolites as Biomarkers in Human Disease

Immobilized Enzymes and Proteins in Urinalysis

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