Levels of 7α‐hydroxy‐4‐cholesten‐3‐one in plasma reflect rates of bile acid synthesis in man

Axelson, Magnus; A, Aly; Sjövall, Jan

doi:10.1016/0014-5793(88)80944-x

Cited by 137 publications

(92 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The coef®cient of variation in duplicate samples was 4.8%. Routine clinical chemistry determination of serum cholesterol levels and cholesterol determined by gas ± liquid chromatography were highly correlated, r Pearson 0.95, P`0.01, 7a-hydroxy-4-cholesten-3-one was determined by HPLC according to Axelson and coworkers (Axelson et al, 1988). The variation coef®cient for duplicate samples was 7%.…”

Section: Methodsmentioning

confidence: 97%

Will recommended changes in fat and fibre intake affect cholesterol absorption and sterol excretion? An ileostomy study

2000

View full text Add to dashboard Cite

Objective: To study cholesterol absorption and excretion in ileostomy subjects with different intakes of saturated fat and dietary ®bre. Design: Short-term experimental study, with four controlled diets in repeated measurements. Setting: Out-patients at metabolic-ward kitchen. Subjects: Nine healthy volunteers with conventional ileostomy after colectomy because of ulcerative colitis. Interventions: Four diet periods, each of 3 days duration: high saturated fat and low dietary ®bre (STAND); reduced saturated fat (RESAT); high saturated fat and high ®bre (FATFIB); and reduced saturated fat and high ®bre (RESATFIB). Main outcome measures: Absorption of cholesterol, and ileal excretion of cholesterol, bile acids, fat and energy. Differences between diets evaluated with Friedman's two-way analysis of variance by rank sum with Bonferroni adjustment, and post hoc differences assessed by rank sum comparison. Results: RESAT and RESATFIB reduced fractional cholesterol absorption by 7% and 10%; RESATFIB and FATFIB increased net cholesterol excretion by 46% and 54% respectively. Further, RESATFIB increased net sterol excretion by 18%, all compared to STAND (P`0.05 for all). All three intervention diets contained more phytosterols than STAND (P`0.05), and the phytosterol content was inversely correlated to fractional cholesterol absorption (r 7 0.77, P`0.01). Conclusions: Current nutrition recommendations to reduce saturated fat and increase dietary ®bre affect sterol excretion additively. The effect on cholesterol absorption might be partly explained by the content of dietary plant sterols. Sponsorship: Supported by grants from the Gothenburg Medical Society, grant numbers 94a086 and 99a082, and by the University of Gothenburg.

show abstract

Section: Methodsmentioning

confidence: 97%

Will recommended changes in fat and fibre intake affect cholesterol absorption and sterol excretion? An ileostomy study

2000

View full text Add to dashboard Cite

show abstract

“…7a-HC in serum, as a measure of 7a-hydroxylase activity and thus of bile acid synthesis, was determined by highperformance liquid chromatography (Axelson et al, 1988) with minor modifications (Andersson et al, 2002). CV from duplicate samples was 7.4%.…”

Section: Methodsmentioning

confidence: 99%

Oat bran rapidly increases bile acid excretion and bile acid synthesis: an ileostomy study

2007

View full text Add to dashboard Cite

Objective: To study whether oat bran with native b-glucans increases bile acid excretion and bile acid synthesis as measured by serum concentrations of 7a-hydroxy-4-cholesten-3-one (7a-HC). Design: Short-term interventional crossover study evaluating cholesterol absorption, ileal excretion of cholesterol and bile acids, and serum levels of cholesterol and bile acid metabolites. Differences between diets evaluated with Wilcoxon's signed rank-sum test.Setting: Outpatients at a metabolic-ward kitchen. Subjects: Nine volunteers with conventional ileostomies. Methods: Two 3-day-diet periods, with controlled, blinded basal diet including 75 g extruded oat bran breakfast cereal daily, with either 11.6 g native or hydrolysed b-glucans. Results: Native oat bran increased median excretion of bile acids by 144% (P ¼ 0.008). Cholesterol excretion remained unchanged, cholesterol absorption decreased by 19% (P ¼ 0.013), whereas the sum of bile acid and cholesterol excretion increased by 40% (P ¼ 0.008) compared with hydrolysed oat bran. 7a-HC reflecting bile acid synthesis increased by 57% (P ¼ 0.008) within 24 h of consumption, whereas serum lathosterol concentration reflecting cholesterol synthesis increased by 12% (P ¼ 0.015). Conclusions: Oat bran with native b-glucans increases bile acid excretion within 24 h of consumption and this increase can also be detected by rising serum concentrations of 7a-HC. Thus, 7a-HC could be used for rapid detection of dietary effects on bile acid metabolism. These effects could possibly be explained by entrapment of whole micelles in the gut owing to higher viscosity.

show abstract

“…Data are expressed as mg/mg Chol. 7a-Hydroxy-4-cholesten-3-one (7a-HCO) was determined in serum using HPLC as previously described by Axelson et al (1988).…”

Section: Laboratory Methodsmentioning

confidence: 99%

Lipoprotein metabolism in patients with anorexia nervosa: a case–control study investigating the mechanisms leading to hypercholesterolaemia

et al. 2004

Self Cite

View full text Add to dashboard Cite

Hypercholesterolaemia is a common finding in patients with anorexia nervosa (AN). To investigate the type, frequency and pathophysiological mechanisms of changes in lipoprotein metabolism in AN we performed a cross-sectional study in fifty-eight female patients (mean age 24·2 years, BMI 15·3 (SD 1·5) kg/m 2 ) and fifty-eight healthy age-matched controls (CO; BMI 22·2 (SD 1·7) kg/m 2 ). Total cholesterol and LDL-cholesterol were higher in AN (5·5 (SD 1·3) v. 5·0 (SD 0·8) mmol/l, P¼0·023; 3·6 (SD 1·1) v. 3·2 (SD 0·7) mmol/l, P¼0·025 respectively). LDL particles were significantly more enriched in cholesterol and triacylglycerol in AN. In multiple regression analysis with LDL-cholesterol as the dependent and BMI, total body fat ( %), lathosterol:cholesterol ratio (endogenous cholesterol synthesis), 7a-hydroxy-4-cholesten-3-one (bile acid synthesis), non-esterified glycerol, free triiodothyronine and free thyroxine as independent variables, BMI was the only significant predictor in CO (R 2 0·36, overall P¼0·001). In AN the variability of LDL-cholesterol was significantly predicted by total body fat, free thyroxine, BMI, free triiodothyronine and non-esterified glycerol (R 2 0·55, overall P,0·001). Subgroup analysis between restricting (AN-R) and binge-eating -purging patients (AN-B) indicated that in AN-R changes in lipoproteins, BMI and total body fat were more pronounced. AN-R patients had lower bile acid synthesis than AN-B (P¼0·02). We conclude that elevated cholesterol concentrations in AN are generally due to an increase in LDL-cholesterol, which is mostly determined by the severe loss of body fat and the resulting changes in thyroid hormones, increased lipolysis and decreased endogenous cholesterol synthesis with resulting decrease in LDL removal. The clinical subtype of AN plays a major role in the mechanisms leading to hypercholesterolaemia.Anorexia nervosa: Lipoprotein metabolism: Hypercholesterolaemia

show abstract

Levels of 7α‐hydroxy‐4‐cholesten‐3‐one in plasma reflect rates of bile acid synthesis in man

Cited by 137 publications

References 15 publications

Will recommended changes in fat and fibre intake affect cholesterol absorption and sterol excretion? An ileostomy study

Will recommended changes in fat and fibre intake affect cholesterol absorption and sterol excretion? An ileostomy study

Oat bran rapidly increases bile acid excretion and bile acid synthesis: an ileostomy study

Lipoprotein metabolism in patients with anorexia nervosa: a case–control study investigating the mechanisms leading to hypercholesterolaemia

Contact Info

Product

Resources

About