Levels of Antibiotic Resistance Genes in Manure, Biosolids, and Fertilized Soil

Munir, Mariya; Xagoraraki, Irene

doi:10.2134/jeq2010.0209

Cited by 205 publications

(110 citation statements)

References 44 publications

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“…Residual solid materials recovered from municipal wastewater treatment systems are rich in microorganisms and a diversity of antibiotic resistance genes (18,21). No more viable enteric bacteria were detected in soil or on vegetables at harvest from plots receiving either biosolids or untreated sewage sludge than in control plots that were fertilized inorganically only (see Tables S4 and S5 in the supplemental material).…”

Section: Discussionmentioning

confidence: 99%

“…Biosolids entrain sorbed antibiotics, including various fluoroquinolones, macrolides, and tetracycline, into soil (17). Soils fertilized with biosolids are enriched with antibiotic resistance genes and with integrons associated with genetic mobility (18,19). There is a risk, therefore, that human consumption of crops grown in ground fertilized with human waste will increase exposure to antibiotic-resistant bacteria and genetic determinants.…”

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confidence: 99%

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Impact of Fertilizing with Raw or Anaerobically Digested Sewage Sludge on the Abundance of Antibiotic-Resistant Coliforms, Antibiotic Resistance Genes, and Pathogenic Bacteria in Soil and on Vegetables at Harvest

Rahube

Marti

Scott

et al. 2014

Appl Environ Microbiol

167

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The consumption of crops fertilized with human waste represents a potential route of exposure to antibiotic-resistant fecal bacteria. The present study evaluated the abundance of bacteria and antibiotic resistance genes by using both culture-dependent and molecular methods. Various vegetables (lettuce, carrots, radish, and tomatoes) were sown into field plots fertilized inorganically or with class B biosolids or untreated municipal sewage sludge and harvested when of marketable quality. Analysis of viable pathogenic bacteria or antibiotic-resistant coliform bacteria by plate counts did not reveal significant treatment effects of fertilization with class B biosolids or untreated sewage sludge on the vegetables. Numerous targeted genes associated with antibiotic resistance and mobile genetic elements were detected by PCR in soil and on vegetables at harvest from plots that received no organic amendment. However, in the season of application, vegetables harvested from plots treated with either material carried gene targets not detected in the absence of amendment. Several gene targets evaluated by using quantitative PCR (qPCR) were considerably more abundant on vegetables harvested from sewage sludge-treated plots than on vegetables from control plots in the season of application, whereas vegetables harvested the following year revealed no treatment effect. Overall, the results of the present study suggest that producing vegetable crops in ground fertilized with human waste without appropriate delay or pretreatment will result in an additional burden of antibiotic resistance genes on harvested crops. Managing human exposure to antibiotic resistance genes carried in human waste must be undertaken through judicious agricultural practice.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Impact of Fertilizing with Raw or Anaerobically Digested Sewage Sludge on the Abundance of Antibiotic-Resistant Coliforms, Antibiotic Resistance Genes, and Pathogenic Bacteria in Soil and on Vegetables at Harvest

Rahube

Marti

Scott

et al. 2014

Appl Environ Microbiol

167

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“…Anthropogenic activities such as RWI and other agricultural practices may contribute to expansion of soil antibiotic resistance (Munir and Xagoraraki, 2011;Cytryn, 2013), and potentially threaten human health through transfer of ARGs and ARB into human-associated pathogens (Forsberg et al, 2012) and food chain (Marti et al, 2013;Wang et al, 2015a). In this study, the numbers and fold changes of the detected ARGs were found to be significantly greater in urban parks with RWI than those without RWI, suggesting that RWI could enhance the diversity and enrichment of soil ARGs.…”

Section: Impacts Of Rwi On the Patterns Of Args In Urban Park Soilsmentioning

confidence: 58%

Impacts of reclaimed water irrigation on soil antibiotic resistome in urban parks of Victoria, Australia

Han

Shi

et al. 2016

Environmental Pollution

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a b s t r a c tThe effluents from wastewater treatment plants have been recognized as a significant environmental reservoir of antibiotics and antibiotic resistance genes (ARGs). Reclaimed water irrigation (RWI) is increasingly used as a practical solution for combating water scarcity in arid and semiarid regions, however, impacts of RWI on the patterns of ARGs and the soil bacterial community remain unclear. Here, we used high-throughput quantitative PCR and terminal restriction fragment length polymorphism techniques to compare the diversity, abundance and composition of a broad-spectrum of ARGs and total bacteria in 12 urban parks with and without RWI in Victoria, Australia. A total of 40 unique ARGs were detected across all park soils, with genes conferring resistance to b-lactam being the most prevalent ARG type. The total numbers and the fold changes of the detected ARGs were significantly increased by RWI, and marked shifts in ARG patterns were also observed in urban parks with RWI compared to those without RWI. The changes in ARG patterns were paralleled by a significant effect of RWI on the bacterial community structure and a co-occurrence pattern of the detected ARG types. There were significant and positive correlations between the fold changes of the integrase intI1 gene and two b-lactam resistance genes (KPC and IMP-2 groups), but no significant impacts of RWI on the abundances of intI1 and the transposase tnpA gene were found, indicating that RWI did not improve the potential for horizontal gene transfer of soil ARGs. Taken together, our findings suggested that irrigation of urban parks with reclaimed water could influence the abundance, diversity, and compositions of a wide variety of soil ARGs of clinical relevance. One-sentence summary: Irrigation of urban parks with treated wastewater significantly increased the abundance and diversity of various antibiotic resistance genes, but did not significantly enhance their potential for horizontal gene transfer.

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“…Third, higher diversity in plant species may create an increased bacterial diversity. Moreover, previous studies found that there were more disturbances in residential soils (Mendes et al, 2011;Munir and Xagoraraki, 2011;Liu et al, 2012).…”

Section: Effect Of Different Land-use On Bacterial Community Compositionmentioning

confidence: 97%

Response of Soil Bacterial Community Structure to Land-use Conversion of Natural Forests in Maoershan National Forest Park, China

Peng¹,

Shah²,

Wang³

et al. 2017

mmr

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To compare and evaluate the soil bacterial community composition in Maoershan National Forest Park, we analyzed soil samples from four replicated land-use types (hardwood forests, cultivated lands, settlement and slash lands) using a denaturing gradient gel electrophoresis (DGGE) method based on 16s rRNA gene fragments. Forty-two DGGE bands were successfully excised for sequencing. Our results revealed that the conversion of natural forest to other land-use types had a significant effect on the soil bacterial community. Bacteroidetes was absent in forest soils. Beta-Proteobacteria was unique to settlement soils, whereas Cyanobacteria and Verrucomicrobia were absent in agricultural soils. Additionally, Acidobacteria and Proteobacteria (α, β, γ, δ classes) were the dominant bacterial communities in all soils. Thus, conversion of the forest land into other land-use types resulted in changes in the bacterial communities which might affect the productivity of the soil ecosystem. Together these results suggested that the utility of using sequence-based approaches to analyze bacterial communities provides detailed information on individual bacterial community composition and permit the robust assessment of the biogeographical patterns.

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Levels of Antibiotic Resistance Genes in Manure, Biosolids, and Fertilized Soil

Cited by 205 publications

References 44 publications

Impact of Fertilizing with Raw or Anaerobically Digested Sewage Sludge on the Abundance of Antibiotic-Resistant Coliforms, Antibiotic Resistance Genes, and Pathogenic Bacteria in Soil and on Vegetables at Harvest

Impact of Fertilizing with Raw or Anaerobically Digested Sewage Sludge on the Abundance of Antibiotic-Resistant Coliforms, Antibiotic Resistance Genes, and Pathogenic Bacteria in Soil and on Vegetables at Harvest

Impacts of reclaimed water irrigation on soil antibiotic resistome in urban parks of Victoria, Australia

Response of Soil Bacterial Community Structure to Land-use Conversion of Natural Forests in Maoershan National Forest Park, China

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