Levels of Antioxidant Compound Glutathione in Moss from Industrial Areas

Varela, Z.; Debén, S.; Saxena, D. K.; Aboal, J.R.; Fernández, J.Á.

doi:10.3390/atmos9070284

Cited by 8 publications

(7 citation statements)

References 36 publications

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“…Para el sitio de Tollocan, que presentó la mayor acumulación de metales en estudio, las briofitas presentaron un aumento del 569 % en la producción de GHS, y en las ubicadas en Oxtotitlán este incremento fue del 116 % respecto de la concentración preexposición (Cuadro IV). Estos resultados concuerdan con lo reportado por Varela et al (2018), quienes encontraron mayor concentración de GHS en los musgos más cercanos a las fábricas, los cuales a su vez presentaron altas concentraciones de Cd, Pb y Ni. Finalmente, para estimar si existe correlación entre la concentración de Cu, Zn y Pb y la concentración-inducción de GSH, se realizaron correlaciones de Spearman entre cada metal y el GSH.…”

Section: Resultados Y Discusiónunclassified

“…Esto es indicativo de que, en presencia de altos niveles de metales, el GHS puede quelarlos y transformarlos en nuevos compuestos menos tóxicos con diferentes propiedades químicas, evitando así el daño celular. Así, este compuesto antioxidante permitiría que el L. angustata mantenga una presencia abundante en la ZMVT (Zepeda- Gómez et al 2012) en sitios altamente contaminados por metales, y pueda ser utilizado para el biomonitoreo de contaminación atmosférica por metales (Saxena y Saxena 2012, Varela et al 2018.…”

Section: Resultados Y Discusiónunclassified

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BIOMONITOREO ACTIVO CON Leskea angustata EN LA ZONA METROPOLITANA DEL VALLE DE TOLUCA

Lara-Almazán

Zarazúa-Ortega

García-Chávez

et al. 2021

RICA

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El objetivo de este trabajo fue evaluar los niveles de Cu, Zn y Pb en la zona metropolitana del valle de Toluca (ZMVT) empleando la técnica de biomonitoreo activo con bolsas con musgo (moss bags) con Leskea angustata como bioindicador, así como determinar si se producen fitoquelatinas (PC) y si hay correlación entre la concentración de éstas y de glutatión (GHS) con los metales presentes en el musgo. Se seleccionaron 10 sitios de monitoreo y en cada uno se colocaron dos bolsas con musgo para ser expuestas al ambiente de la ZMVT durante seis meses. Las concentraciones de metales se determinaron mediante la técnica de fluorescencia de rayos x en reflexión total (TXRF), mientras que el GHS y las PC se cuantificaron empleando la técnica de cromatografía de líquidos de ultra alta resolución acoplada a espectrometría de masas (UPLC-MS). Se evaluó la concentración de metales, PC y GHS antes y después de la exposición y se calculó la tasa de enriquecimiento. Los resultados muestran enriquecimientos de 2 a 30 para metales y de 116 a 569 para GHS. La concentración de PC siempre fue menor al límite de detección. El orden de la concentración total de metales en los sitios de monitoreo fue: Tollocan > Reforma > San Mateo Atenco > Alameda > Aeropuerto > San Cristóbal > Metepec > Ceboruco > Toluca Centro > Oxtotitlán. En general Tollocan, San Mateo Atenco, Alameda y Reforma fueros significativamente diferentes a los demás sitios. El GHS presentó un rango de concentración de 164 a 808 nM/g y presentó correlaciones positivas (r = 0.8) con el Cu, Zn y Pb, por lo que se infiere que la concentración de los metales en estudio tiene relación directa con su inducción. Con los resultados obtenidos en este trabajo se pude concluir que el GHS desempeña un papel fundamental en la bioacumulación de metales divalentes en Leskea angustata de la ZMVT e incrementa su potencial de acumulación, ya que es un mecanismo importante de destoxificación en estos organismos.

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Section: Resultados Y Discusiónunclassified

BIOMONITOREO ACTIVO CON Leskea angustata EN LA ZONA METROPOLITANA DEL VALLE DE TOLUCA

Lara-Almazán

Zarazúa-Ortega

García-Chávez

et al. 2021

RICA

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“…At the same time, other components of the defense system may be involved in counteracting the development of oxidative stress in the gametophytes of moss growing at site 3. In addition to SOD and catalase, the detoxification of ROS and LPO products with the participation of nonenzymatic antioxidants and the activation of the glutathione system are also important links in the cellular defense mechanism [32]. All of these components of the antioxidant system together can participate in the protection of bryo phyte cells from metal toxicity and are important for ensuring their resistance to metal contamination in areas with high traffic loads.…”

Section: Resultsmentioning

confidence: 99%

Accumulation of heavy metals and antioxidant defense system in the gametophyte of Didymodon rigidulus Hedw. in areas with high traffic loads

Polishchuk

Antonyak

2021

Biol. Stud.

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Background. Various modes of transport are among the main sources of environmental pollution with heavy metals and other pollutants. Bryophytes are known to accumulate heavy metals; however, metabolic changes in mosses under conditions of metal accumulation have not been extensively studied. The aim of this article was to analyze the accumulation of heavy metals, the process of lipid peroxidation (LPO) and the activity of enzymes of the antioxidant system in Didymodon rigidulus Hedw. moss collected in urban areas with an intense load created by road and railway transport. Materials and Methods. Gametophyte shoots were collected at three sampling sites in the city of Lviv (Ukraine). Site 1 was selected in a park zone, which was considered a control one; sites 2 and 3 were selected in areas with heavy road and rail traffic, respectively. Concentrations of chromium (Cr), nickel (Ni), lead (Pb), and zinc (Zn) in the moss material were determined by atomic absorption spectrophotometry. The levels of LPO products, namely lipid hydroperoxides and thiobarbituric acid reactive substances (TBARS), as well as superoxide dismutase (SOD) and catalase activities, were determined by standard methods. The results were processed using the methods of variation statistics. Results. Moss D. rigidulus growing in the park area (site 1) accumulated metals in the following order of decreasing concentration: Zn> Cr> Ni> Pb. Gametophyte shoots of D. rigidulus collected at site 2 accumulated higher levels of Pb, Zn and Ni (by 2.27, 1.78 and 1.45 times, respectively), and at site 3, higher levels of Pb and Zn (by 1.8 and 1.67 times, respectively) compared to gametophytes collected in the park zone. In the moss samples from these sites, no significant differences in the Cr content were found as compared to the control. Concentration of lipid hydroperoxides in the moss sampled at sites 2 and 3 was 4.26 and 3.75 times higher, respectively, compared to the control, and TBARS production was more intense in plant material from site 2 than from the control site. SOD and catalase activities were considerably increased in D. rigidulus moss from site 2 compared with those from the control area; however, the activity of both enzymes in the moss samples from site 3 did not significantly exceed the control levels. Conclusions. Both road and rail traffic loads contribute to the accumulation of heavy metals, especially Zn and Pb, in D. rigidulus moss growing in the surrounding areas. Under such conditions, LPO process is stimulated, which is more pronounced in moss growing in area with heavy road traffic. The increased activity of antioxidant enzymes (SOD and catalase) in moss growing in this area can play an important role in protecting bryophyte cells against metal-induced oxidative stress under conditions of intense metal accumulation.

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“…The glutathione-ascorbate cycle is one of the major antioxidant mechanisms in cells. Ascorbate peroxidase (APX) and glutathione reductase (GR) activities were shown to increase in response to different types of stress exposure in plants, and it was confirmed by analyzing the mosses [59,60] growing near metal-polluted industrial areas. We did not find synthesis of phytochelatins (PCs), heavy-metal binding peptides, in T. barbieri plants.…”

Section: Role Of Antioxidants In Cu Tolerance/toxicitymentioning

confidence: 86%

Mechanisms of Copper Toxicity and Tolerance in the Aquatic Moss Taxiphyllum barbieri

Bačkor,

Goga,

Singh

et al. 2023

Plants

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Aquatic habitats are very frequently polluted with different kinds of xenobiotics, including heavy metals. For biomonitoring studies of aquatic pollution, algae are frequently used, as they do not contain protective cuticle on the surface of their thalli and can accumulate pollutants over the whole surface of thalli. However, this is a feature of most cryptogams. For this reason, we assessed the sensitivity of the aquatic moss Taxiphyllum barbieri (Java moss) to copper excess in a short-term study. Moss T. barbieri belongs to the common aquatic plants originating from Southeast Asia. To test the sensitivity (or tolerance) of the moss to excess Cu, selected concentrations (50, 250 and 500 µM) were employed in our 24 h studies. Total and intracellular Cu accumulation positively correlated with Cu availability in the water. This total and intracellular Cu accumulation was negatively correlated with decreased intracellular K content. Excess Cu negatively affected the composition of assimilation pigments and soluble proteins. Cu caused increased peroxidation of membrane lipids assessed using TBARS assay. Excess Cu decreased GSH to GSSG ratio and ascorbic acid content. We did not observe phytochelatin synthesis in this moss. The roles of selected amino acids, their intermediates and derivatives, as well as S-containing nucleosides and phenolic acids in Cu homeostasis and toxicity or tolerance were evaluated. We assume that this moss has potential for future employment in water quality evaluation.

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Levels of Antioxidant Compound Glutathione in Moss from Industrial Areas

Cited by 8 publications

References 36 publications

BIOMONITOREO ACTIVO CON Leskea angustata EN LA ZONA METROPOLITANA DEL VALLE DE TOLUCA

BIOMONITOREO ACTIVO CON Leskea angustata EN LA ZONA METROPOLITANA DEL VALLE DE TOLUCA

Accumulation of heavy metals and antioxidant defense system in the gametophyte of Didymodon rigidulus Hedw. in areas with high traffic loads

Mechanisms of Copper Toxicity and Tolerance in the Aquatic Moss Taxiphyllum barbieri

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