Levels of aqueous humor trace elements in patients with open-angle glaucoma

Hohberger, Bettina; Chaudhri, M. Anwar; Michalke, Bernhard; Lucio, Marianna; Nowomiejska, Katarzyna; Schlötzer‐Schrehardt, Ursula; Grieb, Paweł; Ręjdak, Robert; Jünemann, Anselm

doi:10.1016/j.jtemb.2017.10.003

Cited by 49 publications

(37 citation statements)

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“…The essential metals Cu and Zn play vital roles in retinal cell survival and are crucial for the normal functioning of antioxidant enzymes. Considering oxidative stress, iron as Fe(II), promotes formation of free radicals, especially in the retina [18,19]. From our data, we found no evidence of abnormal Zn in CMVR patients, at least in this patient population.…”

Section: Discussionmentioning

confidence: 45%

Abnormal levels of aqueous humor trace elements in patients with cytomegalovirus retinitis

Zhou

Wang

et al. 2019

Eye

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Purpose To investigate the alterations of trace elements levels in aqueous humor of patients with cytomegalovirus retinitis (CMVR). Methods A total of 15 eyes of 11 patients with CMVR and 24 eyes of 24 patients with senile cataract as control group were enrolled. Aqueous humor samples were assessed for calcium (Ca), potassium (K), magnesium (Mg), sodium (Na), phosphorus (P), titanium (Ti), vanadium (V), chromium (Cr), manganese (Mn), iron (Fe), nickel (Ni), copper (Cu), zinc (Zn), selenium (Se), strontium (Sr), and lead (Pb) by using inductively coupled-plasma-mass-spectrometry. Meanwhile, we examined the concentration of the CMV DNA load by using PCR and the concentration of interleukin (IL)-8 by using a cytometric bead array. Results In patients with CMVR, the aqueous humor levels of P and Cu were significantly higher than those of controls (p < 0.001, p < 0.001, respectively). However, levels of K and Mg were significantly lower in patients with CMVR (p < 0.001, p < 0.001, respectively). The Spearman correlation test showed that the concentration of IL-8 in the aqueous humor was significantly associated with the aqueous level of Cu (p = 0.009, r = 0.646) and Se (p = 0.031, r = 0.558). In addition, the concentration of CMV DNA load in the aqueous humor was significantly associated with the aqueous level of Ca (p = 0.027, r = −0.568), Mn (p = 0.020, r = 0.593), and Cu (p = 0.043, r = 0.527). Conclusions Our preliminary results demonstrated that the abnormal aqueous levels of trace elements (P and Cu) in CMVR patients. Thus, the roles of trace element changes in the development of CMVR and the influence of intraocular trace element for the prognosis of CMVR warrant further investigations.

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Section: Discussionmentioning

confidence: 45%

Abnormal levels of aqueous humor trace elements in patients with cytomegalovirus retinitis

Zhou

Wang

et al. 2019

Eye

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“…The pathophysiology of glaucoma is known to be multifactorial. In addition to oxidative stress (2), vascular dysregulation (3), or morphological alterations of the trabecular meshwork (4), several further factors may be involved. Intraocular pressure (IOP) is the main risk factor for glaucoma.…”

Section: Introductionmentioning

confidence: 99%

Autoantibodies Activating the β2-Adrenergic Receptor Characterize Patients With Primary and Secondary Glaucoma

et al. 2019

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Recently, agonistic autoantibodies (agAAb) activating the β2-adrenergic receptor were detected in primary open-angle glaucoma (POAG) or ocular hypertension (OHT) patients and were linked to intraocular pressure (IOP) (1). The aim of the present study was to quantify β2-agAAb in the sera of glaucoma suspects and patients with primary and secondary glaucoma. Patients with OHT (n = 33), pre-perimetric POAG (pre-POAG; n = 11), POAG (n = 28), and 11 secondary OAG (SOAG) underwent ophthalmological examinations including examinations with Octopus G1 perimetry and morphometry. Twenty-five healthy individuals served as controls. Serum-derived IgG samples were analyzed for β2-agAAb using a functional bioassay. The beat-rate-increase of spontaneously beating cultured neonatal rat cardiomyocytes was monitored with 1.6 beats/15 s as cut-off. None of the sera of normal subjects showed β2-agAAb. In POAG or OHT patients increased beating rates of 4.1 ± 2.2 beats/15 s, and 3.7 ± 2.8 beats/15 s were detected (p > 0.05). Glaucoma patients with (POAG) and without perimetric (pre-POAG) defects did not differ (pre-POAG 4.4 ± 2.6 beats/15 s, POAG 4.1 ± 2.0 beats/15 s, p > 0.05). Patients with SOAG yielded mean beating rates of 4.7 ± 1.7 beats/15 s (p > 0.05). β2-agAAb were seen in 73% of OHT, 82% of pre-POAG, 82% of POAG, and 91% SOAG patients (p < 0.001). Clinical data did not correlate with beating rate (p > 0.05). The robust β2-agAAb seropositivity in patients with OHT, pre-POAG, POAG, and SOAG suggest a primary common role for β2-agAAb starting early in glaucoma pathophysiology and turned out to be a novel marker identifying all patients with increased IOP independent of glaucoma stage and entity.

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“…ICP-MS experiments showed that mice with glaucoma had lower retinal Fe, Mg, Ca, Mn and Zn concentrations than pre-glaucomatous mice. More recently, the aqueous humor levels of cadmium, iron, manganese, cobalt, copper and zinc were measured in the aqueous humor samples of patients with primary open-angle glaucoma (POAG) and pseudo-exfoliation glaucoma (PEXG) by Flow-Injection-Inductively-Coupled-Plasma-Mass-Spectrometry (FI-ICP-MS) [28]. It was found that there were significant differences in the aqueous humor levels of zinc and iron between glaucoma and control patients.…”

Section: Introductionmentioning

confidence: 99%

Levels of Trace Elements in the Aqueous Humor of Cataract Patients Measured by the Inductively Coupled Plasma Optical Emission Spectrometry

et al. 2019

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Trace elements play an important role in the pathogenesis of several serious ophthalmological disorders, such as glaucoma, age-related macular degeneration (AMD), diabetic retinopathy, cataract, etc. This study aimed to measure alterations of chemical elements’ (67) levels in the aqueous humor of patients undergoing cataract surgery. The pilot study included 115 patients, (age 74 ± 7.27, female 64.35%, male 35.65%). The aqueous levels of elements were measured by the use of the inductively coupled plasma optical emission spectrometry (ICP-OES), quality controlled with certified standards. The classification of elements based on their concentration was achieved by hierarchical cluster analysis. This is the first screening study that quantifies over 60 elements which are present in the fluid from the anterior chamber of the eye of cataract patients. The obtained results can be suitable for understanding and identifying the causes that may play a role in the initiation and progression of lens opacity.

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Levels of aqueous humor trace elements in patients with open-angle glaucoma

Cited by 49 publications

References 50 publications

Abnormal levels of aqueous humor trace elements in patients with cytomegalovirus retinitis

Abnormal levels of aqueous humor trace elements in patients with cytomegalovirus retinitis

Autoantibodies Activating the β2-Adrenergic Receptor Characterize Patients With Primary and Secondary Glaucoma

Levels of Trace Elements in the Aqueous Humor of Cataract Patients Measured by the Inductively Coupled Plasma Optical Emission Spectrometry

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