Levels of glycolytic enzymes and metabolites in the red blood cells of the red-legged pademelon, Thylogale stigmatica (macropodidae: marsupialia)

Agar, N.S.; Spencer, Peter B. S.

doi:10.1016/0305-0491(93)90188-b

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…However, the exception was the bridled nailtail wallaby (Table 3). Very high lactate production rates with autologous plasma have previously been seen in the red legged pademelon (Agar and Spencer, 1993a) and the spectacled hare-wallaby (Agar and Spencer, 1993b). RBC of eutherian mammals have also been shown to produce large quantities of lactate when incubated with their own plasma (Kim, 1983).…”

Section: Resultsmentioning

confidence: 88%

Comparative erythrocyte metabolism in marsupials and monotremes

Parkinson

Whittington

Spencer

et al. 1995

Comparative Biochemistry and Physiology Part C: Pharmacology, T

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Concentrations of ATP and DPG, activities of 10 enzymes and the glycolytic rates were measured in the erythrocytes of 11 species of marsupials and two species of monotremes. Mean DPG concentrations were greater in the erythrocytes of marsupials than those of eutherian mammals. The opposite is true of ATP. Significant findings from the results of enzyme activities were: high activity of hexokinase (7.39 ± 0.82 EU/g Hb) in the short-beaked echidna, pyruvate kinase (37.49 ± 1.0 EU/g) Hb in bridled nailtail wallaby and glucose-6-phosphate dehydrogenase (G6PD; 41.66 ± 1.24 EU/g Hb) in black-striped wallaby. About 6-to 7-fold difference in the activity of G6PD levels between the two species of wombats was confirmed. Glucose phosphate isomerase activity was also shown to be twice as high in the red cells of the common wombat compared with those of the southern hairy nosed wombat. There were wide variations in the glycolytic rate among the species examined.

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Section: Resultsmentioning

confidence: 88%

Comparative erythrocyte metabolism in marsupials and monotremes

Parkinson

Whittington

Spencer

et al. 1995

Comparative Biochemistry and Physiology Part C: Pharmacology, T

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“…GST activity was especially low in the RBC of the allied rock wallaby (0.77 ± 0.11 EU/g Hb) and the southern hairy nosed wombat (0.83 ± 0.13 EU/g Hb). Low GST activity has also been reported in one other marsupial, the red-legged pademelon (0.290 ± 0.023 EU/g Hb) (Agar and Spencer, 1993). Why these species should have such low levels of GST in their erythrocytes is unknown.…”

Section: Glutathione-s-transferase (Gst)mentioning

confidence: 95%

“…GSH-Px activity varies significantly between different eutherian and marsupial species (Harvey and Kaneko, .1975;Maral et at., 1977;Suzuki, 1982;Wightman et at., 1986;Agar and Godwin, 1991;Agar and Spencer, 1993;Kurata et at., 1993b). In the present study, considerable variation was also observed in the RBC of the species studied.…”

Section: Glutathione Peroxidase (Gsh-px)mentioning

confidence: 99%

Comparative study of the antioxidant defence systems in the erythrocytes of australian marsupials and monotremes

Whittington

Parkinson

Spencer

et al. 1995

Comparative Biochemistry and Physiology Part C: Pharmacology, T

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A comparison of the erythrocyte (RBC) antioxidant metabolites and enzymes in nine marsupial and two monotreme species was carried out. Reduced glutathione (GSH) concentrations were comparable with those reported for other marsupial and eutherian species. An important finding was that the erythrocytes of the southern hairy nosed wombat regenerated GSH faster than the erythrocytes from its close relative, the common wombat. The activities of glutathione-S-transferase, NADH-methaemoglobin reductase, superoxide dismutase, and glutathione peroxidase (GSH-Px), showed similar levels and extents of variation as those observed in other marsupial and eutherian species. Catalase activities in the marsupials were lower than those measured in the two monotreme species and much lower than those reported in eutherian species. A negative correlation, significant at P < 0.05, was observed between GSH-Px and catalase activities in the RBC of the marsupials. Since both these enzymes "detoxify" H 2 0 2 , there appears to be a reciprocal relationship between the activities of these enzymes in marsupial RBC.

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