Levels of IgE in serum from normal children and allergic children as measured by an enzyme immunoassay

Lindberg, Roger E.; Arroyave, Carlos M.

doi:10.1016/0091-6749(86)90078-3

Cited by 56 publications

(31 citation statements)

References 23 publications

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“…No sex influence on the IgE levels could be demonstrated in the current study, a finding that concurs with some reports 29,32,38 and conflicts with others. 30,[39][40][41] …”

Section: Discussionsupporting

confidence: 77%

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Serum Polyclonal Immunoglobulin E Levels among an Apparently Healthy Iraqi Population: A Preliminary Report

Al-Diwan¹,

Al-Hadithi²,

Al‐Balaghi

et al. 1989

Ann Saudi Med

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The distribution of serum polyclonal IgE levels as determined by paper radioimmunosorbent test was studied in 150 nonallergic, presumably healthy individuals. Prior to natural logarithmic conversion, the distribution was strongly skewed to the left, and normal distribution followed logarithmic conversion. The range of serum polyclonal IgE levels was 1 to 2439 μg/L with a geometric mean value of 197 μg/L. Regression analysis of natural logarithm of serum polyclonal IgE levels with age revealed no significant association. Higher levels were demonstrated among individuals 10 to 14 years old than among other age groups. Variation of IgE levels among individuals and patients stemmed from differences in race, 24 age and sex, 6,25,26 assay methods, 27 and other factors including infections. Discrepancies in various studies may be due to poor definition of the study population, variability of environmental factors, use of nonstandardized reagents, and different statistical methods.This study is a preliminary report on the serum polyclonal IgE levels in a nonallergic presumably healthy Iraqi urban population. Materials and MethodsVenous blood samples were obtained from 150 apparently healthy subjects attending Al-Waih Pediatric Hospital to vaccinate their children. They gave no history of allergy, and their stool and urine examination revealed no helminthic infection. The mean age of these individuals was 26 ± 14.5 years (ranging from 8 months to 65 years) Serum Polyclonal Immunoglobulin E Levels Among an Apparently Healthy Iraqi Population: A Preliminary Report Annals of Saudi Medicine, Vol 9 No. 2; 1989with a male-to-female ratio of 1.3:1. Sera were separated from clotted blood by cen-trifugation, divided into aliquots, and stored at -20°C. Serum polyclonal IgE levels were determined by paper radioimmunosorbent test as described by Gleich et al, 28 using commercially available kits. Serum samples were assayed in duplicate within the same batch, and the values recorded were the mean of two determinations. In brief, anti-IgE coated filter paper disks were incubated overnight with test serum. After three washings, each disk was incubated with anti-IgE-125 I. After three more washings, the radioactivity percent on each disk was measured in a gamma counter. Results were expressed as percentage binding (the ratio between count per minutes of each disk and total radioactivity).The results were evaluated statistically by the Student t test and analysis of variance. P values less than 0.05 were considered statistically significant. ResultsThe frequency distribution of serum polyclonal IgE was strongly skewed to the left (Figure 1) with almost 31.3% of values below 120 μg/L. The data were transformed to natural logarithm to get a normal distribution (Figure 2).

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“…No sex influence on the IgE levels could be demonstrated in the current study, a finding that concurs with some reports 29,32,38 and conflicts with others. 30,[39][40][41] …”

Section: Discussionsupporting

confidence: 77%

“…It has predictive values in small children with hereditary predisposition as well. 29 Several authors have attempted to define normal levels and ranges in nonatopic "healthy" populations. However, the precise normal levels of serum IgE are difficult to determine as there are several factors affecting the level.…”

Section: Discussionmentioning

confidence: 99%

Serum Polyclonal Immunoglobulin E Levels among an Apparently Healthy Iraqi Population: A Preliminary Report

Al-Diwan¹,

Al-Hadithi²,

Al‐Balaghi

et al. 1989

Ann Saudi Med

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“…Some authors have found that there is a higher risk for atopic disease when IgE concentrations exceed 10 IU?mL -1 during the first year of life [8]. In a cohort study of allergic and nonallergic children from 1 yr until adolescence, significant differences were observed in IgE levels between the two groups up to 11 yrs of age [9]. In the present study, the mean IgE levels were significantly higher in infants with recurrent wheezing in all periods between birth and 1 yr of age.…”

Section: Discussionmentioning

confidence: 99%

Are immunoglobulin E levels associated with early wheezing? A prospective study in Brazilian infants

López¹,

Barros‐Mazon²,

Vilela³

et al. 2002

Eur Respir J

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The relationship between wheezing or asthma and serum immunoglobulin (Ig)E levels in early life is unclear. The aim of this study was to follow-up the IgE immune response in infants that did, or did not, develop recurrent wheezing during their first year of life.One-hundred and two randomised term neonates were included, in which IgE levels were quantified in cord blood samples, at 3, 6, 9 and 12 months of age. Specific IgE levels for food and inhalant allergens and the skin-prick test for inhalant allergens were also assessed at 6 and 12 months.During clinical follow-up, 32 (31%) infants presented with two or more wheezing episodes, while 70 (69%) had no wheezing. Total IgE levels were significantly higher up to 12 months in wheezing infants when compared to nonwheezing group. At 12 months, the specific IgE levels to cow9s milk, egg white and mites were higher in infants with recurrent wheezing. There was no influence of family history for atopy on IgE levels. The skin-prick tests were positive in 14% and 23% in wheezing infants at 6 and 12 months, respectively.These results indicate an early allergic sensitisation in wheezing infants, suggesting an altered immunoregulatory T-cell role in immunoglobulin E production. Eur Respir J 2002; 20: 640-645.

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“…While the low IgE levels observed in North America and Europe are consistent with low levels of helminth exposure, individuals in these populations with allergic diseases such as asthma do show elevated IgE levels (e.g., Bergmann et al 1995 ;Holford-Strevens et al 1984 ;Lindberg and Arroyave 1986 ). Variations on the "hygiene" and "old friends" hypotheses posit that a major factor in the rise of allergic, autoimmune, and infl ammatory disorders in industrialized populations today is a mismatch between a human immune system that coevolved with "old friends" (e.g., helminths and commensals) and a modern hygienic environment in which these "old friends" are largely absent (Rook 2008 ).…”

Section: Helminth-induced Immune Responses and Coinfection In Humansmentioning

confidence: 92%

Make New Friends and Keep the Old? Parasite Coinfection and Comorbidity in Homo sapiens

Martin

Blackwell

Gurven

et al. 2013

Primates, Pathogens, and Evolution

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Levels of IgE in serum from normal children and allergic children as measured by an enzyme immunoassay

Cited by 56 publications

References 23 publications

Serum Polyclonal Immunoglobulin E Levels among an Apparently Healthy Iraqi Population: A Preliminary Report

Serum Polyclonal Immunoglobulin E Levels among an Apparently Healthy Iraqi Population: A Preliminary Report

Are immunoglobulin E levels associated with early wheezing? A prospective study in Brazilian infants

Make New Friends and Keep the Old? Parasite Coinfection and Comorbidity in Homo sapiens

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