Levels of insulin-like growth factor-binding protein-2 and insulin-like growth factor-II in maternal serum, amniotic fluid and extraembryonic coelomic fluid at 9–20 weeks of pregnancy

Chard, T.; Blum, W. F.; Brunjes, J; Campbell, Denise; Wathen, N. C.

doi:10.1677/joe.0.1420379

Cited by 16 publications

(13 citation statements)

References 15 publications

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“…3 It is found in much higher concentrations in the fetal circulation, suggesting a vital role in augmenting the fetal response to IGF-I and, particularly, IGF-II. 3 The fall in maternal IGFBP-2 concentrations from pre-conception to 12 weeks of gestation shown in our study has also been reported by Chard et al 40 Adjusting IGFBP-2 concentrations for haemodilution in the same manner as for IGF-II has a profound e¡ect on the changes during pregnancy. Absolute values show a signi¢cant fall in IGFBP-2 from conception until the second trimester, followed by a small increase that does not return to pre-conception values at any point.…”

Section: Discussionsupporting

confidence: 88%

Longitudinal changes of insulin-like growth factors and their binding proteins throughout normal pregnancy

et al. 2004

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Section: Discussionsupporting

confidence: 88%

Longitudinal changes of insulin-like growth factors and their binding proteins throughout normal pregnancy

et al. 2004

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“…Conversely, in human, mid-trimester amniotic fluid levels of CBG are much lower than even nonpregnant serum levels [24]. Steroid and peptide hormones [25, 26]and various proteins [27, 28]in human amniotic fluid originate from the decidua and chorionic trophoblast and not the mother. Sex hormone-binding globulin (SHBG) is also well known as a specific steroid-binding protein for androgen and estrogen [29], and is regulated differently in the amniotic and maternal compartments [30].…”

Section: Discussionmentioning

confidence: 99%

Evidence for the Synthesis of Corticosteroid-Binding Globulin in Human Placenta

Misao¹,

Iwagaki²,

Sun³

et al. 1999

Horm Res Paediatr

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We demonstrated the expression of corticosteroid-binding globulin (CBG) in human placenta using reverse transcription-polymerase chain reaction-Southern blot analysis and immunohistochemical and immunoblotting studies. In the RT-PCR-Southern blot analysis, only one predicted PCR product was detected without nonspecific products in all samples of human placenta and 3A (tPA-30-1) human placental cells. In Western blot analysis, polyclonal anti-CBG antibodies recognized a protein of approximately 55 kD in the protein extracts prepared from 3A (tPA-30-1) cells. Additionally, CBG mRNA expression was demonstrated by in situ hybridization in the syncytiotrophoblasts. Immunohistochemical studies performed on the placenta demonstrated the presence of specific immunoreactivity in the syncytiotrophoblast layer surrounding the chorionic villi. These findings suggest that CBG is synthesized in human placenta during pregnancy in addition to its synthesis in the liver.

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“…The control groups included 25 healthy children (age 3.0-17.1 years, average 9.3 years) as well as 15 children at their initial Radioimmunoassay (RIA) IGFBP-1, -2 and -3 were determined by radioimmunoassay as described previously (Blum et al, 1990;Chard et al, 1994).…”

Section: Materials and Methods Patient Samplesmentioning

confidence: 99%

Elevated serum levels of IGFBP-2 found in children suffering from acute leukaemia is accompanied by the occurrence of IGFBP-2 mRNA in the tumour clone

Wex

Vorwerk²,

Mohnike³

et al. 1998

Br J Cancer

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Summary Insulin-like growth factor-binding proteins (IGFBPs) are important modulators of IGF action. In 50 children suffering from acute lymphoblastic leukaemia (ALL), we studied the serum levels of IGFBP-1,-2 and-3. The mean standard deviation score (SDS) values were estimated to be 0.7, 3.1 and -1.7 for the IGFBP-1,-2 and-3, respectively, compared with the normal range defined by a SDS from -2 to +2. IGFBP-1 and-3 were normal, but for IGFBP-2 we found a significantly elevated serum level compared with control groups (P < 0.05). However, during chemotherapy this increased serum IGFBP-2 normalized. In addition, we found a correlation between higher serum levels and the detection rate of the IGFBP-2 transcript in corresponding cells. In patients with ALL, the detection rates of IGFBP-2 mRNA were estimated to be 72% and 35% at the time of diagnosis and at day 33 of chemotherapy respectively; in the control groups (healthy children and children at their initial presentation of diabetes mellitus), the values were 28% and 33% respectively. Based on the correlation between IGFBP-2 serum levels and the corresponding gene expression as well as the normalization of IGFBP-2 levels during chemotherapy, we concluded that the increased serum level mainly originated from the tumour clone itself. Furthermore, possible functional consequences of elevated IGFBP-2 were outlined.

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Levels of insulin-like growth factor-binding protein-2 and insulin-like growth factor-II in maternal serum, amniotic fluid and extraembryonic coelomic fluid at 9–20 weeks of pregnancy

Cited by 16 publications

References 15 publications

Longitudinal changes of insulin-like growth factors and their binding proteins throughout normal pregnancy

Longitudinal changes of insulin-like growth factors and their binding proteins throughout normal pregnancy

Evidence for the Synthesis of Corticosteroid-Binding Globulin in Human Placenta

Elevated serum levels of IGFBP-2 found in children suffering from acute leukaemia is accompanied by the occurrence of IGFBP-2 mRNA in the tumour clone

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