Levels of protein fractions in blood serum of periparturient goats

Abstract

The aim of the study was to compare contents of individual protein fractions determined by electrophoresis in blood serum of healthy periparturient goats. Eight clinically healthy white shorthaired goats were examined. Blood samples of these goats were taken from v. jugularis three weeks and two weeks before the anticipated parturition, on the parturition day, 7 days after the parturition, and 28 days after the parturition. Individual protein fractions, albumins, α1-, α2-, β1-, β2-and γ-globulins were identified by electrophoretic analysis of blood serum. Percentage shares of individual protein fractions were converted from total protein values to g·l -1 values. The results indicated that individual serum protein fractions in periparturient goats showed significant dynamics of change. There were no significant differences between samples taken before the parturition and on the parturition day (except for the increase of β2 from 2.5 ± 0.3 g·l -1 observed 3 weeks before the parturition to 3.2 ± 0.4 g·l -1 measured 2 weeks before the parturition, and the decrease of γG levels from 9.5 ± 2.6 g·l -1 observed three weeks before the parturition to 8.1 ± 1.7 g·l -1 found on the parturition day), yet most indicators measured after the parturition were significantly higher (p < 0.05) than on the parturition day (values in g·l -1 observed on the parturition day versus values measured 28 days after the parturition: total protein 60.6 ± 4.3 vs. 71.3 ± 2.4, albumins 37.4 ± 2.9 vs. 42.7 ± 1.0, α2 3.4 ± 0.5 vs. 4.1 ± 0.8, β1 5.6 ± 0.5 vs. 6.3 ± 0.9, γG 8.1 ± 1.7 vs. 12.3 ± 1.9). The results bring new knowledge on the values of individual fractions of serum proteins in healthy goats, which is necessary for diagnostic interpretation of pathological findings in animals and also for experimental studies.

Hormonal Profile, Antioxidant Status and Some Biochemical Parameters during Pregnancy and Periparturient Period in Dromedary She Camel

“…Serum globulin showed no significant alterations around parturition, this result disagreed with most findings as Janku et al [63] because this period could be associated mainly with the production of colostrum and other immunological changes typical for the periparturient period but this observation agreed with Saleh et al [61]. The change of total protein, albumin and globulin may be due to the transfer of albumin and γ-globulins and total protein from blood to the mammary glands [64].…”

Serum proteins and their diagnostic utility in veterinary medicine: a review

“…Variations in the serum protein profile were found in ewes during pregnancy and lactation, as well as in periparturient goats (Janku et al 2011;Chiaradia et al 2012;Piccione et al 2012b). Changes in the concentrations of protein fractions during the last phase of pregnancy and early postpartum in dairy cows are less well documented.…”

“…On the other hand, variations in the serum protein profile and concentrations may occur also under some physiological conditions (Weaver et al 2000;Janku et al 2011).…”

Hemato-Biochemical Profiling in Relation to Metabolic Disorders in Transition Dairy Cows

“…On other hand potassium level not changed in three groups, Serum total proteins as parturition approach usually decreased may be due to the fetus synthesizes all its proteins from the amino acids derived from the dam and fetal muscle growth reaching its maximum size at late pregnancy (Jainudee, and Hafez, 1994). Serum globulins showed a significant decrease during transition period, which associated mainly with the production of colostrum that rich in antibodies that derived from γ-globulins and other immunological changes occurred around parturition (Janku et al, 2011). Serum total protein, albumin and urea are indicators of diet protein intake (Toharmat et al, 1999).…”