Levels of steroidogenic acute regulatory protein and mitochondrial membrane potential in granulosa cells of older poor-responder women

Fatum, Muhammad; Yivgi-Ohana, Natalie; David, Samuel; Orly, Joseph; Simon, Alexander; Laufer, Neri

doi:10.1016/j.fertnstert.2007.10.027

Cited by 10 publications

(4 citation statements)

References 23 publications

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“…3 Mitochondrial gene expression ratio in patients who received hMG was statistically higher than that of patients under recFSH granulosa cells that contain substantially lower levels of normal mitochondria as compared with women 34 years or younger [23,24]. Moreover, Fatum et al [25] hypothesized that during the process of reproductive aging, mitochondrial membrane potential may deteriorate as a result of free-radical accumulation.…”

Section: Discussionmentioning

confidence: 99%

Difference in mitochondrial gene expression in granulosa cells between recombinant FSH and hMG cycles under in vitro fertilization and transfer

Saito

Yamashita

Higuchi

et al. 2013

Reprod Medicine & Biology

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Purpose Examination of the mitochondrial mRNA expression in granulosa cells from an unspecified population of infertile patients to evaluate whether recombinant follicle stimulating hormone (recFSH) is more effective in producing higher quality embryo rates compared with human menopausal gonadotropin (hMG). Method Thirty-nine patients who underwent the in vitro fertilization and embryo transfer program were retrospectively examined. Patients were administered recFSH (n = 18) or hMG (n = 20) in a long protocol where GnRH agonist was used. Granulosa cells were obtained during oocyte retrieval and examined for mitochondria mRNA expression ratio against GAPDH. Expressions of mitochondria mRNA were evaluated by real-time PCR analysis. Results The high-quality embryo rate in the hMG cycle was higher than in the recFSH cycle, and the total dose of hMG showed a positive correlation with the expression level of mitochondrial genes in granulosa cells. Moreover, mitochondria mRNA expression was higher in the hMG cycle than in the recFSH cycle. Conclusions Compared with recFSH, hMG induces a higher mitochondrial gene expression ratio in granulosa cells at the time of oocyte retrieval and, therefore, may lead to higher quality embryo rates.

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Section: Discussionmentioning

confidence: 99%

Difference in mitochondrial gene expression in granulosa cells between recombinant FSH and hMG cycles under in vitro fertilization and transfer

Saito

Yamashita

Higuchi

et al. 2013

Reprod Medicine & Biology

View full text Add to dashboard Cite

show abstract

“…Additionally, POR patients tend to have lower peak levels of estradiol (E2) and fewer granulosa cells than young women with normal fertility. The situation leads to significantly lower pregnancy rates per ovarian pickup when compared to normal responders in the same age group [60][61][62]. These findings emphasize the importance of exploring strategies that improve not only the quality of oocytes but also the overall ovarian environment in the development of treatments for women facing infertility due to aging.…”

Section: The Process Of Ovarian Decay In Aging Women Is a Multi-facto...mentioning

confidence: 98%

Facilitation of Ovarian Response by Mechanical Force—Latest Insight on Fertility Improvement in Women with Poor Ovarian Response or Primary Ovarian Insufficiency

Chang

2023

IJMS

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The decline in fertility in aging women, especially those with poor ovarian response (POR) or primary ovarian insufficiency (POI), is a major concern for modern IVF centers. Fertility treatments have traditionally relied on gonadotropin- and steroid-hormone-based IVF practices, but these methods have limitations, especially for women with aging ovaries. Researchers have been motivated to explore alternative approaches. Ovarian aging is a complicated process, and the deterioration of oocytes, follicular cells, the extracellular matrix (ECM), and the stromal compartment can all contribute to declining fertility. Adjunct interventions that involve the use of hormones, steroids, and cofactors and gamete engineering are two major research areas aimed to improve fertility in aging women. Additionally, mechanical procedures including the In Vitro Activation (IVA) procedure, which combines pharmacological activators and fragmentation of ovarian strips, and the Whole Ovary Laparoscopic Incision (WOLI) procedure that solely relies on mechanical manipulation in vivo have shown promising results in improving follicle growth and fertility in women with POR and POI. Advances in the use of mechanical procedures have brought exciting opportunities to improve fertility outcomes in aging women with POR or POI. While the lack of a comprehensive understanding of the molecular mechanisms that lead to fertility decline in aging women remains a major challenge for further improvement of mechanical-manipulation-based approaches, recent progress has provided a better view of how these procedures promote folliculogenesis in the fibrotic and avascular aging ovaries. In this review, we first provide a brief overview of the potential mechanisms that contribute to ovarian aging in POI and POR patients, followed by a discussion of measures that aim to improve ovarian folliculogenesis in aging women. At last, we discuss the likely mechanisms that contribute to the outcomes of IVA and WOLI procedures and potential future directions.

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“…( 19 ) demonstrated lower StAR expression and P4 production in the luteinized GCs of patients with DOR. In addition, it was previously reported that granulosa cells of elderly women (38-41 years) display defective mitochondria and fewer lipid droplets than the cells of a younger group ( 20 , 21 ) and a reduced mitochondrial DNA content ( 22 ). Therefore, it might be speculated that ovarian hypo-response to stimulation of young women reflects an ovarian premature senescence.…”

Section: Relationship Between Ovarian Sensitivity To Fsh and Steroido...mentioning

confidence: 99%

Subtle perturbations of ovarian steroidogenesis in patients classified as Poseidon Group 3. Which consequences for therapeutic strategy?

Hugues

2024

Front. Endocrinol.

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The optimal strategy for stimulation of young women with a low ovarian reserve is still a challenging issue because the physio-pathogeny of this disorder is often unknown. As androgen production by the ovary plays a crucial role in folliculogenesis, it was tempting to speculate that subtle perturbations in ovarian steroidogenesis might participate to the low responsiveness to gonadotrophins. Indeed, in vitro analysis of human luteinized granulosa cells has recently provided evidence for some enzymatic deficits in steroidogenesis and altered response to gonadotrophins. Therefore, improving androgen environment of women classified in Poseidon Group 3 should be considered. In this clinical situation, the potential benefit of androgen supplementation or stimulation of theca cells by LH-activity products are respectively discussed.

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Levels of steroidogenic acute regulatory protein and mitochondrial membrane potential in granulosa cells of older poor-responder women

Cited by 10 publications

References 23 publications

Difference in mitochondrial gene expression in granulosa cells between recombinant FSH and hMG cycles under in vitro fertilization and transfer

Difference in mitochondrial gene expression in granulosa cells between recombinant FSH and hMG cycles under in vitro fertilization and transfer

Facilitation of Ovarian Response by Mechanical Force—Latest Insight on Fertility Improvement in Women with Poor Ovarian Response or Primary Ovarian Insufficiency

Subtle perturbations of ovarian steroidogenesis in patients classified as Poseidon Group 3. Which consequences for therapeutic strategy?

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