Levels of the human hepatocyte growth factor in serum of patients with various liver diseases determined by an enzyme-linked immunosorbent assay

Tsubouchi, Hirohito; Niitani, Yoshiyuki; Hirono, Shuichi; Nakayama, Hiroyuki; Gohda, Eiichi; Arakaki, Naokatu; Sakiyama, Osami; Takahashi, Kozo; Kimoto, Masayoshi; Kawakami, Shuichi; Setoguchi, Minami; Tachikawa, Tetsuya; Shin, Sadahito; Arima, Terukatsu; Daikuhara, D.D.S. Yasushi

doi:10.1002/hep.1840130102

Cited by 290 publications

(117 citation statements)

References 10 publications

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“…This is consistent with data showing that all the other MET mutants preserve their responsiveness to the ligand Michieli et al, 1999). Since the MET receptor ligand is present in plasma and tissues (Masumoto and Yamamoto, 1991;Sonnenberg et al, 1993;Tsubouchi et al, 1991), ligand availability is probably not a limiting step to activation.…”

Section: Discussionsupporting

confidence: 85%

Somatic mutations of the MET oncogene are selected during metastatic spread of human HNSC carcinomas

et al. 2000

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A metastatic cancer develops by accumulation of mutations in genes that control growth, survival and spreading. The latter genes have not yet been identi®ed. In lymph node metastases of head and neck squamous cell carcinomas (HNSCC), we found mutations in the MET oncogene, which encodes the tyrosine kinase receptor for Scatter Factor, a cytokine that stimulates epithelial cell motility and invasiveness during embryogenesis and tissue remodeling. We identi®ed two somatic mutations: the Y1230C, known as a MET germline mutation which predisposes to hereditary renal cell carcinoma, and the Y1235D that is novel and changes a critical tyrosine, known to regulate MET kinase activity. The mutated MET receptors are constitutively active and confer an invasive phenotype to transfected cells. Interestingly, cells carrying the MET mutations are selected during metastatic spread: transcripts of the mutant alleles are highly represented in metastases, but barely detectable in primary tumors. These data indicate that cells expressing mutant MET undergo clonal expansion during HNSCC progression and suggest that MET might be one of the long sought oncogenes controlling progression of primary cancers to metastasis. Oncogene (2000) 19, 1547 ± 1555.

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Section: Discussionsupporting

confidence: 85%

Somatic mutations of the MET oncogene are selected during metastatic spread of human HNSC carcinomas

et al. 2000

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“…The ELISA described in this report is a sandwich method 20 and is highly specific for mature (active) HGF and does not cross-react to the single-chain form of HGF. 7 After vascular damage, enzymatically active thrombin is produced and is incorporated into the thrombus.…”

Section: Hgf and Coagulationmentioning

confidence: 99%

Prognostic Value of Serum Hepatocyte Growth Factor in Patients With Acute Coronary Syndromes

et al. 1999

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“…26 HGF messenger RNA in the fulminant hepatitis phase, fell in the chronic hepa-(mRNA) is expressed in various tissues. 27 It has already been titis phase, and was intermediate or high during the hepreported that HGF production increases in rats injured by atoma phase.…”

Section: Cinoma (Hcc) Cells and The Cytoplasm Of The Nonepithe-mentioning

confidence: 99%

Hepatocyte Growth Factor and C– Met Expression in Long–Evans Cinnamon Rats With Spontaneous Hepatitis and Hepatoma

et al. 1996

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mal accumulation of copper in the liver of LEC rats, 40 times The Long-Evans Cinnamon (LEC) rat is characterized greater than that in control Long-Evans Agouti rats, whereas by the spontaneous onset of acute and chronic hepatitis, the levels of ceruloplasmin in LEC rat serum were markedly followed by occurrence of liver cancer, and is thus able low. [4][5] It is worth noting that these features of the liver disorto provide a unique experimental model for human geder in LEC rats are very similar to those that characterize netical liver disease, Wilson's disease. HepatocyteWilson's disease. [4][5][6][7] Recently, Wu et al. 8 cloned complemengrowth factor (HGF) is a potent hepatotrophic factor in tary DNAs for a rat gene (Atp7b) homologous to the human liver regeneration, and its expression is up-regulated inWilson's disease gene (ATP7B) and identified a partial deleresponse to liver injuries. We found that the plasma HGF tion in the Atp7b gene in the LEC rat. level in LEC rats rose markedly during the fulminant Hepatocyte growth factor (HGF) was first identified in the hepatitis phase, fell during the phase of chronic/cholanserum of partially hepatectomized rats 9 and purified from rat giofibrosis, and fluctuated during the hepatoma phase.platelets 10 and human plasma. 11,12 HGF is a ligand for the cImmunohistological staining of the liver revealed that met protooncogene product of receptor-type tyrosine kinase, 13-17 the number of HGF-positive cells increased remarkably and is considered to be a major hepatotrophic factor in the during the fulminant hepatitis phase, and that many of induction of mitosis of hepatocytes during liver regenerathese cells were localized at the portal triads. Fewer tion. [18][19][20] Acting as a motogen, HGF induces cell move-HGF-positive cells were observed during the phase of ment, 21,22 and, while acting as a morphogen, it induces the chronic hepatitis. The surface of the hepatocellular carformation of tissue-like structures [23][24][25] for various cell types. cinoma (HCC) cells and the cytoplasm of the nonepithe-The serum levels of HGF are elevated not only in human lial cells in cancerous liver tissues were HGF-positive.patients with fulminant hepatitis, but also in those with The HGF-messenger RNA (mRNA) level in the liver rose chronic hepatitis and cirrhosis. 26 HGF messenger RNA in the fulminant hepatitis phase, fell in the chronic hepa-(mRNA) is expressed in various tissues. 27 It has already been titis phase, and was intermediate or high during the hepreported that HGF production increases in rats injured by atoma phase. The expression of c-met mRNA was strong hepatectomy or CCl 4 , and that nonparenchymal cells produce in the tissues of LEC rats with fulminant hepatitis and, Both the levels of HGF mRNA and the activity of especially, in the cholangiofibrosis tissues. c-met mRNA HGF increase prior to liver regeneration. 28 It has also been was also detected in HCCs. These results suggest that reported that recombinant HGF has promoted remarkable the HGF-c-met system may pla...

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Levels of the human hepatocyte growth factor in serum of patients with various liver diseases determined by an enzyme-linked immunosorbent assay

Cited by 290 publications

References 10 publications

Somatic mutations of the MET oncogene are selected during metastatic spread of human HNSC carcinomas

Somatic mutations of the MET oncogene are selected during metastatic spread of human HNSC carcinomas

Prognostic Value of Serum Hepatocyte Growth Factor in Patients With Acute Coronary Syndromes

Hepatocyte Growth Factor and C– Met Expression in Long–Evans Cinnamon Rats With Spontaneous Hepatitis and Hepatoma

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