Leverage principle of retardation signal in titration of double protein via chip moving reaction boundary electrophoresis

Zhang, Liu Xia; Cao, Yi Ren; Xiao, Hua; Liu, Peter; Liu, Shao Rong; Meng, Qing Hua; Fan, Liu Yin; Cao, Cheng Xi

doi:10.1016/j.bios.2015.09.001

Cited by 23 publications

(15 citation statements)

References 38 publications

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“…Herein, we outlined the electrophoresis titration (ET) model of counting ligands capped on NPs by relying on the protein ET, , enzyme catalysis ET, and photocatalysis ET via moving reaction boundary (MRB). Figure and Figure S1 show the NPs-ET model for counting the ligands on the NPs surface.…”

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Facile Counting of Ligands Capped on Nanoparticles via a Titration Chip of Moving Reaction Boundary Electrophoresis

et al. 2019

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Absolute quantification of ligand capped on the surface of nanoparticles (NPs) has faced a great challenge without the use of complex inner standards (CIS). Herein, we proposed a facile electrophoresis titration (ET) model, designed an ET device, and developed a relevant method for counting the ligand on NPs without the use of CIS, based on moving reaction boundary (MRB). Furthermore, we conducted the relevant ET runs by using 3-mercaptopropionic acid (MPA) and quantum dots (QDs) as the model ligand and NPs, respectively. The experiments revealed that the ligand content of 1518 ± 295 obtained via an ET was close to the one of 1408 ± 117 determined via NMR, validating the ET model. Moreover, the experiments showed fair stability (RSD < 5.62%) and simplicity of ET without the use of CIS. Evidently, the ET model opens a window for facile assay of ligand capped on NPs.

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“…Reaction creates a sharp anodic-moving acid–base neutralized MRB, which can be observed via the phenolphthalein indicator in the channel. In the model, there is always a balance between the number of ligands titrated and the equivalent amount of titrant consumed − (see the Supporting Information).…”

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Facile Counting of Ligands Capped on Nanoparticles via a Titration Chip of Moving Reaction Boundary Electrophoresis

et al. 2019

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“…Protein is the basic unit that makes up any living organism. Protein and amino acid detection are crucial in medical diagnosis (Yoo et al, 2014), food security (Setford et al, 2002), and biotechnology (Zhang et al, 2016). These molecules are also compatible with 2D materials as nucleic acids.…”

Section: Other Chemical Sensingmentioning

confidence: 99%

Chemical Sensors Based on Two-Dimensional (2D) Materials for Selective Detection of Ions and Molecules in Liquid

2019

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Up until now, two-dimensional (2D) materials have been researched vigorously for application to sensing ions and molecules in liquid due to their unique structural, chemical, and electronic properties. Features of 2D materials such as high surface area-to-volume ratios and various reaction sites are ideal characteristics for fabricating state-of-the-art high-performed chemical sensors. This review particularly focuses on the detection of pH, metal ions, and biomolecules in liquid media. The final goal of the ion/molecule sensors is a development of the electronic tongue or taste sensors that can be used in medical, food, biotechnology, and health applications. Herein, we introduce recent advances in the field of ion/molecule sensors in liquid media based on 2D materials, especially concentrating in graphene and MoS2, and will emphasize the opportunities and challenges of these unique sensing materials and devices.

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“…Protein, an important component in human cells and tissues, participates in the physiological activities of the body, catalyzes the cell processes and plays important roles in the research of bioengineering, medical diagnosis, treatment and proteomics [ 1 , 2 , 3 ]. Some protein receptors can specifically bind to small organic molecules [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Label-Free Fluorescence Molecular Beacon Probes Based on G-Triplex DNA and Thioflavin T for Protein Detection

Xue

Zhou

2021

Molecules

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Protein detection plays an important role in biological and biomedical sciences. The immunoassay based on fluorescence labeling has good specificity but a high labeling cost. Herein, on the basis of G-triplex molecular beacon (G3MB) and thioflavin T (ThT), we developed a simple and label-free biosensor for protein detection. The biotin and streptavidin were used as model enzymes. In the presence of target streptavidin (SA), the streptavidin hybridized with G3MB-b (biotin-linked-G-triplex molecular beacon) perfectly and formed larger steric hindrance, which hindered the hydrolysis of probes by exonuclease III (Exo III). In the absence of target streptavidin, the exonuclease III successively cleaved the stem of G3MB-b and released the G-rich sequences which self-assembled into a G-triplex and subsequently activated the fluorescence signal of thioflavin T. Compared with the traditional G-quadruplex molecular beacon (G4MB), the G3MB only needed a lower dosage of exonuclease III and a shorter reaction time to reach the optimal detection performance, because the concise sequence of G-triplex was good for the molecular beacon design. Moreover, fluorescence experiment results exhibited that the G3MB-b had good sensitivity and specificity for streptavidin detection. The developed label-free biosensor provides a valuable and general platform for protein detection.

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Leverage principle of retardation signal in titration of double protein via chip moving reaction boundary electrophoresis

Cited by 23 publications

References 38 publications

Facile Counting of Ligands Capped on Nanoparticles via a Titration Chip of Moving Reaction Boundary Electrophoresis

Facile Counting of Ligands Capped on Nanoparticles via a Titration Chip of Moving Reaction Boundary Electrophoresis

Chemical Sensors Based on Two-Dimensional (2D) Materials for Selective Detection of Ions and Molecules in Liquid

Label-Free Fluorescence Molecular Beacon Probes Based on G-Triplex DNA and Thioflavin T for Protein Detection

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