Leveraging the biosorption potential of Leptolyngbya boryana for Cr (VI) removal from aqueous solution

Yadav, Ajit Pratap Singh; Goswami, Lalit; Dwivedi, Vinay; Kumar, Satyendra

doi:10.1016/j.clet.2021.100198

Cited by 21 publications

(7 citation statements)

References 44 publications

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“…We previously characterized these strains morphologically and biochemically. 17 Pathogenic and antibiotic-treated L. boryana strains were excluded from this study.…”

Section: Collection Of Samplesmentioning

confidence: 99%

“…The morphology (colony morphology and color identification) of isolated L. boryana strains was characterized as described previously. 17 For Cr reductase analysis, isolated and morphologically characterized L. boryana strains were inoculated in a large volume (5 L) of BG-II medium and maintained at 37°C for 10 days with constant agitation in at 100 rpm. L. boryana colonies were harvested by centrifugation (1000 rpm at 4°C for 30 min).…”

Section: Morphological and Biochemical Analysismentioning

confidence: 99%

“…16 In our previous study, we reported the successful isolation and characterization of L. boryana from tannery soils. 17 In the present study, we aimed to characterize the Cr reductase (ChrR) gene and investigate its variability in the context of Cr (VI) stress. The findings of this study may provide a more suitable, effective, eco-friendly, sustainable, and cost-effective biological treatment of wastewater from the leather industry.…”

Section: Introductionmentioning

confidence: 99%

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ChrR Gene Variability in Cr-stressed Leptolyngbya boryana for the Biotransformation of Cr (VI) to Cr (III)

Yadav¹,

Dwivedi²,

Kumar³

et al. 2023

J. Pure Appl. Microbiol.

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Chromium (VI) is a well-known pollutant that is present in industrially polluted soil and water, and has been reported to be mutagenic and carcinogenic. In the present study, we investigated the effective use of Leptolyngbya boryana (cyanobacterium) as an eco-friendly option to overcome Cr (VI) toxicity in tannery effluents. The main objective of this study was to identify the Cr reductase (ChrR) gene and its variability in the context of Cr (VI) stress. Industrial polluted soil samples were collected and processed according to standard protocols for ChrR variation and 16S rDNA gene analysis. Genomic DNA was isolated from the collected samples and the ChrR and 16S rDNA genes were amplified by PCR. Amplified 16S rDNA was sequenced and aligned with known sequences. In the present study, a strong correlation was established between the nucleotide sequences of the ChrR and 16S rDNA genes. The Minimum Inhibitory Concentration (MIC) was determined for Cr (VI), and pure strains of L. boryana were identified and isolated from soil samples. Cr (VI)-stressed conditions and their genetic variability were confirmed by sequencing. In conclusion, the L. boryana strain has been identified an eco-friendly option for overcoming Cr (VI) toxicity in tannery effluents.

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“…We previously characterized these strains morphologically and biochemically. 17 Pathogenic and antibiotic-treated L. boryana strains were excluded from this study.…”

Section: Collection Of Samplesmentioning

confidence: 99%

Section: Morphological and Biochemical Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ChrR Gene Variability in Cr-stressed Leptolyngbya boryana for the Biotransformation of Cr (VI) to Cr (III)

Yadav¹,

Dwivedi²,

Kumar³

et al. 2023

J. Pure Appl. Microbiol.

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“…The screened Leptolyngbya boryana strains were grown in the Erlenmeyer asks containing liquid BG-II media with growth conditions: 16:8 light: dark cycle; 30 ± 2°C and the 6 irradiances of 3000-4000 lux (cool white light). Further, the isolates were routinely cultured and maintained via sub-culturing under its metabolically active state after every 20 days as mentioned previously (Yadav et al, 2021).…”

Section: Cyanobacteria and Culture Conditionsmentioning

confidence: 99%

“…The morphology (colony morphology and colour identi cation) of isolated Leptolyngbya boryana strains were performed as described previously (Yadav et al, 2021). The biochemical estimation of Gram's stain, Catalase activity, Sucrose utility and Indole test were also performed in all strains as described (Sundari et al, 2013).…”

Section: Morphological and Biochemical Analysismentioning

confidence: 99%

Genetic Variability Screening of the Leptolyngbya Boryana with Expressed ChrR Gene for the Biotransformation of Cr (VI) to Cr (III) Reduction

Yadav¹,

Dwivedi²,

Kumar³

2021

Preprint

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Chromium is well known pollutant for its mutagenicity, and carcinogenicity in humans. Excessive uses of chromium in leather tanning industries, stainless-steel production, and wood preservatives have resulted as chromium contamination in soil and water. This investigation indicates the effective use of Leptolyngbya boryana as an eco-friendly option to overcome Chromium (VI) toxicity in tannery effluents. The main objective of this research was to find out ChrR gene and its variability in the context of Cr (VI) stress. This is a novel study in the relation of Leptolyngbya boryana. Industrial polluted soil samples were collected and processed according to the standard protocols for ChrR variation and 16S rRNA gene. DNA was isolated and amplified through PCR. Amplified DNA was sequenced and aligned with the known sequences. In this study a strong co-relation was established in the nucleotide sequences of ChrR and 16S rRNA genes. MIC was determined for Cr (VI) and pure strains of Leptolyngbya boryana were identified and isolated from soil. In the present study presence of ChrR gene variability was recorded in Leptolyngbya boryana which is a cyanobacterium in the soil of tannery effluent under Cr (VI) stressed condition and its gene variability was confirmed by sequencing. We can conclude that Leptolyngbya boryana strain could be eco-friendly option to overcome Chromium (VI) toxicity in tannery effluents.

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Reduction of Hexavalent Chromium [Cr(VI)] by Heavy Metal Tolerant Bacterium Alkalihalobacillus clausii CRA1 and Its Toxicity Assessment Through Flow Cytometry

2021

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Leveraging the biosorption potential of Leptolyngbya boryana for Cr (VI) removal from aqueous solution

Cited by 21 publications

References 44 publications

ChrR Gene Variability in Cr-stressed Leptolyngbya boryana for the Biotransformation of Cr (VI) to Cr (III)

ChrR Gene Variability in Cr-stressed Leptolyngbya boryana for the Biotransformation of Cr (VI) to Cr (III)

Genetic Variability Screening of the Leptolyngbya Boryana with Expressed ChrR Gene for the Biotransformation of Cr (VI) to Cr (III) Reduction

Reduction of Hexavalent Chromium [Cr(VI)] by Heavy Metal Tolerant Bacterium Alkalihalobacillus clausii CRA1 and Its Toxicity Assessment Through Flow Cytometry

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