Levetiracetam-mediated improvement of decreased NMDA-induced glutamate release from nerve terminals during hypothermia

Pastukhov, Artem; Borisova, Tatiana

doi:10.1016/j.brainres.2018.06.032

Cited by 10 publications

(6 citation statements)

References 40 publications

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“…It has also been found that sCT reduces intracellular calcium levels in response to high potassium stimulation (Welch and Olson 1991). Calcium plays a vital role in glutamate-induced cytotoxicity (Pastukhov and Borisova 2018). The current study found that sCT raised the cell viability and inhibited apoptosis after glutamate-induced cytotoxicity in C6 cells.…”

Section: Discussionsupporting

confidence: 63%

The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play

Taşkıran

Ergül

2021

Metab Brain Dis

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Recent evidence has shown that salmon calcitonin (sCT) has positive effects on the nervous system.However, its effect and mechanisms on glutamate-induced cytotoxicity are still unclear. The current experiment was designed to examine the effect of sCT on glutamate-induced cytotoxicity in C6 cells, involving the in ammatory and nitric oxide stress pathways. The study used the C6 glioma cell line. Four cell groups were prepared to evaluate the effect of sCT on glutamate-induced cytotoxicity. The control group was without any treatment. Cells in the glutamate group were treated with 10 mM glutamate for 24 h. Cells in the sCT group were treated with various concentrations (3, 6, 12, 25, and 50 µg/mL) of sCT for 24 hours. Cells in the sCT + glutamate group were pre-treated with various concentrations of sCT for 1 hour and then exposed to glutamate for 24 hours. The cell viability was evaluated with an XTT assay. Nuclear factor kappa b (NF-kB), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), neuronal nitric oxide synthase (nNOS), nitric oxide (NO), cyclic guanosine monophosphate (cGMP), caspase-3, and caspase-9 levels in the cells were measured by ELISA kits. Apoptosis was detected by ow cytometry method. sCT at all concentrations signi cantly improved the cell viability in C6 cells after glutamateinduced cytotoxicity (p < 0.001). Moreover, sCT signi cantly reduced the levels of NF-kB (p < 0.001), TNFα, and IL-6 levels (p < 0.001). sCT also decreased nNOS, NO, and cGMP levels (P < 0.001). Furthermore, it decreased the apoptosis rate and increased the live-cell rate in the ow cytometry (P < 0.001). In conclusion, sCT has protective effects on glutamate-induced cytotoxicity in C6 glial cells by inhibiting in ammatory and nitric oxide pathways. sCT could be a useful supportive agent for people with neurodegenerative symptoms.

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Section: Discussionsupporting

confidence: 63%

The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play

Taşkıran

Ergül

2021

Metab Brain Dis

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“…LEV had no effect on AMPA-induced glutamate release [29], which may explain that AMPAR was not involved in LEV-induced theta activity. Whereas others reported that LEV reduced the EPSC amplitude via affecting the presynaptic voltage-dependent calcium channel [30].…”

Section: Discussionmentioning

confidence: 88%

“…Thus, we currently have no clear explanation about why LEV-induced theta was not involved in AMPAR. NMDAR involvement of LEV-induced theta may be explained by the observation that LEV increased selectively NMDA-induced glutamate release [29].…”

Section: Discussionmentioning

confidence: 99%

Levetiracetam induction of theta frequency oscillations in rodent hippocampus in vitro

Xing

Xie

et al. 2020

Can. J. Physiol. Pharmacol.

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Levetiracetam(LEV) has been demonstrated to improve cognitive function. Hippocampal theta rhythm (4–12 Hz) is associated with a variety of cognitively related behaviors, such as exploration in both humans and animal models. We investigated the effects of LEV on the theta rhythm in the rat hippocampal CA3 in hippocampal slices in vitro. We found that LEV increased the theta power in a dose-dependent manner. The increase in theta power can be blocked by GABAA receptor(GABAAR) or NMDA receptor(NMDAR) antagonists but not by AMPA receptor antagonist, indicating the involvement of GABAAR and NMDAR in the induction of theta activity. Interestingly, LEV enhancement of theta power can be also blocked by taurine or GABA-A agonist THIP, indicating that LEV induction of theta may be related to the indirect boosting of GABA action via reduction of extrasynaptic GABAAR activation. Furthermore, the increased theta power can be partially reduced by mACh receptor(mAChR) antagonist atropine but not by nACh receptor antagonists, suggesting that mAChR activation provides excitatory input into local network responsible for LEV induced theta. Our study demonstrated that LEV induced a novel theta oscillation in vitro, which may have implications in treatment of the neuronal disorders with impaired theta oscillation and cognitive function.

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“…Wistar rats, males, with a body weight of 250–300 g, were kept in animal facilities of the Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, as described in [ 22 ] according to the European guidelines, international laws, and policies. Rats were housed in a temperature-controlled room (22–23 °C) and provided with water and dry food pellets ad libitum.…”

Section: Methodsmentioning

confidence: 99%

Transient coating of γ-Fe₂O₃ nanoparticles with glutamate for its delivery to and removal from brain nerve terminals

Paliienko¹,

Pastukhov²,

Babič³

et al. 2020

Beilstein J. Nanotechnol.

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Glutamate is the main excitatory neurotransmitter in the central nervous system and excessive extracellular glutamate concentration is a characteristic feature of stroke, brain trauma, and epilepsy. Also, glutamate is a potential tumor growth factor. Using radiolabeled ʟ-[14C]glutamate and magnetic fields, we developed an approach for monitoring the biomolecular coating (biocoating) with glutamate of the surface of maghemite (γ-Fe2O3) nanoparticles. The nanoparticles decreased the initial rate of ʟ-[14C]glutamate uptake, and increased the ambient level of ʟ-[14C]glutamate in isolated cortex nerve terminals (synaptosomes). The nanoparticles exhibit a high capability to adsorb glutamate/ʟ-[14C]glutamate in water. Some components of the incubation medium of nerve terminals, that is, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and NaH2PO4, decreased the ability of γ-Fe2O3 nanoparticles to form a glutamate biocoating by about 50% and 90%, respectively. Only 15% of the amount of glutamate biocoating obtained in water was obtained in blood plasma. Albumin did not prevent the formation of a glutamate biocoating. It was shown that the glutamate biocoating is a temporal dynamic structure at the surface of γ-Fe2O3 nanoparticles. Also, components of the nerve terminal incubation medium and physiological fluids responsible for the desorption of glutamate were identified. Glutamate-coated γ-Fe2O3 nanoparticles can be used for glutamate delivery to the nervous system or for glutamate adsorption (but with lower effectiveness) in stroke, brain trauma, epilepsy, and cancer treatment following by its subsequent removal using a magnetic field. γ-Fe2O3 nanoparticles with transient glutamate biocoating can be useful for multifunctional theranostics.

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Levetiracetam-mediated improvement of decreased NMDA-induced glutamate release from nerve terminals during hypothermia

Cited by 10 publications

References 40 publications

The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play

The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play

Levetiracetam induction of theta frequency oscillations in rodent hippocampus in vitro

Transient coating of γ-Fe₂O₃ nanoparticles with glutamate for its delivery to and removal from brain nerve terminals

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Levetiracetam-mediated improvement of decreased NMDA-induced glutamate release from nerve terminals during hypothermia

Cited by 10 publications

References 40 publications

The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play

The effect of salmon calcitonin against glutamate-induced cytotoxicity in the C6 cell line and the roles the inflammatory and nitric oxide pathways play

Levetiracetam induction of theta frequency oscillations in rodent hippocampus in vitro

Transient coating of γ-Fe2O3 nanoparticles with glutamate for its delivery to and removal from brain nerve terminals

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Product

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Transient coating of γ-Fe₂O₃ nanoparticles with glutamate for its delivery to and removal from brain nerve terminals