Levetiracetam normalisiert die Connexin 43 Expression und die interzelluläre Kommunikation in primären Astrozytenkulturen

Ladage, Kerstin; Haghikia, Aiden; Hinkerohe, Daniel; Smikalla, D.; Dermietzel, Rolf; Faustmann, Pedro M.

doi:10.1055/s-2004-833116

Cited by 1 publication

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“…Expression of Cx43 was determined by using a rank scale, which consisted of four defined ranks: 25%, 50%, 75%, and 100% Cx43 positivity per culture (Faustmann al., 2003). The Cx43 expression detected by immunocytochemical labeling was validated by Western blotting (data not shown) (Ladage et al, 2004).…”

Section: Immunofluorescencementioning

confidence: 99%

Effects of cytokines on microglial phenotypes and astroglial coupling in an inflammatory coculture model

Hinkerohe

Smikalla

Haghikia

et al. 2005

Glia

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Cytokines play an important role in the onset, regulation, and propagation of immune and inflammatory responses within the central nervous system (CNS). The main source of cytokines in the CNS are microglial cells. Under inflammatory conditions, microglial cells are capable of producing pro- and antiinflammatory cytokines, which convey essential impact on the glial and neuronal environment. One paramount functional feature of astrocytes is their ability to form a functionally coupled syncytium. The structural link, which is responsible for the syncytial behavior of astrocytes, is provided by gap junctions. The present study was performed to evaluate the influence of inflammation related cytokines on an astroglial/microglial inflammatory model. Primary astrocytic cultures of newborn rats were cocultured with either 5% (M5) or 30% (M30) microglial cells and were incubated with the following proinflammatory cytokines: tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), interferon-gamma (IFN-gamma), and the antiinflammatory cytokines transforming growth factor-beta1 (TGF-beta1) and IFN-beta. Under these conditions, i.e., incubation with the inflammatory cytokines and the high fraction of microglia (M30), microglial cells revealed a significant increase of activated round phagocytotic cells accompanied by a reduction of astroglial connexin 43 (Cx43) expression, a reduced functional coupling together with depolarization of the membrane resting potential (MRP). When the antiinflammatory mediator TGF-beta1 was added to proinflammatory altered M30 cocultures, a reversion of microglial activation and reconstitution of functional coupling together with recovery of the astroglial MRP was achieved. Finally IFN-beta, added to M5 cocultures was able to prevent the effects of the proinflammatory cytokines TNF-alpha, IL-1beta, and IFN-gamma.

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Section: Immunofluorescencementioning

confidence: 99%