Lewinella agarilytica sp. nov., a novel marine bacterium of the phylum Bacteroidetes, isolated from beach sediment

Abstract

Lewinella agarilytica sp. nov., a novel marine bacterium of the phylum Bacteroidetes, isolated from beach sediment Soon Dong LeeCorrespondence Soon Dong Lee sdlee@cheju.ac.kr Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea A Gram-negative, aerobic, light-orange-coloured, marine bacterium (designated SST-19 T ) was isolated from beach sediment in Jeju, Korea, and its taxonomic position was determined by means of a polyphasic approach. In a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain SST-19 T was positioned within the family 'Saprospiraceae', class Sphingobacteria, and formed a consistent cluster with Lewinella persica ATCC 23167 T (94.7 % sequence similarity). The levels of 16S rRNA gene sequence similarity between the isolate and recognized members of the family ranged from 84 to 89 %, with Lewinella cohaerens ATCC 23123 T , Lewinella nigricans ATCC 23147 T , Haliscomenobacter hydrossis DSM 1100 T and Saprospira grandis ATCC 23119 T showing values of 89.0 , 88.4 , 86.4 and 84.1 %, respectively. Strain SST-19 T required seawater for growth and its cells were unbranched, flexible rods or filaments with gliding motility. The cellular fatty acids consisted mainly of saturated, unsaturated and iso-branched fatty acids, the major components being C 16 : 1 v7c and/or iso-C 15 : 0 2-OH (24.1 %), C 18 : 0 (13.5 %), iso-C 15 : 0 (11.1 %) and C 16 : 0 (10.9 %). The G+C content of the DNA was found to be 51.3 mol%. On the basis of the results from phenotypic and phylogenetic analyses, the isolate represents a novel species of the genus Lewinella, for which the name Lewinella agarilytica sp. nov. is proposed. The type strain is

Variability and abundance of the epiphytic bacterial community associated with a green marine Ulvacean alga

“…Bacteroidetes bacteria have also been linked with the maintenance of normal thallus structure in the green alga Monostroma oxyspermum (Matsuo et al, 2003), although bacteria from a range of phyla including the Proteobacteria have also been shown to induce normal morphology in the green alga Ulva linza (Marshall et al, 2006). Six of the seven Bacteroidetes sequences are closely related to the Saprospiraceae family, strains of which have been isolated from seawater (Lee, 2007;Oh et al, 2009) and algae (Hosoya et al, 2006). Saprospiraceae sequences have also been detected in a previous study of U. australis and the red alga Delisea pulchra (Longford et al, 2007).…”

Portibacter lacus gen. nov., sp.nov., a new member of the family Saprospiraceae isolated from a saline lake

“…The G+C content of the genomic DNA of the type strain is 53.7 mol%. The predominant cellular fatty ac- (Yoon et al, 2011); 3, Lewinella cohaerens II-2 T (Khan et al, 2007); 4, Lewinella nigricans SS-2 T (Khan et al, 2007); 5, Lewinella persica T-3 T (Khan et al, 2007); 6, Lewinella agarilytica SST-19 T (Lee, 2007); 7, Lewinella antarctica IMCC3223 T (Oh et al, 2009); 8, Lewinella lutea FYK2402M69 T (Khan et al, 2007); 9, Lewinella marina MKG-38 T (Khan et al, 2007); 10, Haliscomenobacter hydrossis ATCC 27775 T (van Veen et al, 1973;Mulder, 1989); 11, Aureispira marina 24 T (Hosoya et al, 2006); 12, Aureispira maritima 59SA T (Hosoya et al, 2007); 13, Saprospira grandis ATCC 23119 T (Reichenbach, 1989). Symbols: +, positive; , negative; v, variable; ND, no data.…”

“…It includes the genera Saprospira (type genus), Haliscomenobacter, Lewinella, Aureispira and Rubidimonas . The genera Saprospira, Haliscomenobacter and Rubidimonas each accommodate one species, Saprospira grandis (Reichenbach, 1989), Haliscomenobacter hydrossis (van Veen et al, 1973) and Rubidimonas crustatorum (Yoon et al, 2011) and the genus Lewinella accommodates seven species, Lewinella agarilytica (Lee, 2007), Lewinella antarctica (Oh et al, 2009), Lewinella cohaerens (Khan et al, 2007), Lewinella lutea (Khan et al, 2007), Lewinella marina (Khan et al, 2007), Lewinella nigricans (Khan et al, 2007) and Lewinella persica (Khan et al, 2007). The genus Aureispira (Hosoya et al, 2006) comprises two species, Aureispira marina (Hosoya et al, 2006) and Aureispira maritima (Hosoya et al, 2007).…”

Lewinella antarctica sp. nov., a marine bacterium isolated from Antarctic seawater

“…L. cohaerens, L. nigricans and L. persica, isolated from littoral sediment samples and originally assigned to the genus Herpetosiphon by Lewin (1970), were later transferred to the new genus Lewinella on the basis of data from 16S rRNA gene sequence analyses (Sly et al, 1998). L. agarilytica (Lee, 2007) and L. lutea (Khan et al, 2007) were isolated from shallow marine sediment samples, whilst L. marina (Khan et al, 2007) was isolated from a sea snail. The members of the genus Lewinella are generally characterized as being Gram-negative, aerobic, flexirubin-negative, chemoheterotrophic rods or filaments (up to 25 mm long).…”