Licht- und elektronenmikroskopische Cytochemie der unreifzelligen Leuk�mien

Huhn, D.; Schmalzl, F.

doi:10.1007/bf01497569

Cited by 10 publications

(3 citation statements)

References 32 publications

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“…This isoenzyme is characterized by a high substrate specificity for naphthol-AS-BI-phosphate, which was used as a substrate in our investigations. In addition, some undissolved material which did not invade the gel was detected in cases of T-ALL; this suggests the occurrence of a membrane bound isoenzyme which might be responsible for the strong focal cytochemical reaction partly confined to the Golgi region in this subtype of ALL (Huhn & Schmalzl 1972, Schmalzl et a1 1974, Catovsky et a1 1978a.…”

Section: Discussionmentioning

confidence: 95%

“…Distinct and coarsely granular PAS-positivity in ALL is caused by deposits of glycogen. In addition, a faint granular positivity may be provoked by intracytoplasmic granules containing a finely granular proteinaceous content in electron microscopic preparations and when investigated in simultaneous light microscopic-cytochemical and electron microscopic preparations of leukaemic blasts (Huhn & Schmalzl 1972, Schmalzl et a1 1974.…”

Section: Discussionmentioning

confidence: 99%

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Cytochemistry and Membrane Markers in Acute Lymphatic Leukaemia (ALL)

Huhn¹,

Thiel

Rodt

et al. 1981

Scandinavian Journal of Haematology

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136 patients suffering from ALL were subdivided into 5 subtypes (C‐ALL, C/T‐ALL, pre‐T‐ALL, B‐ALL) according to rosetting tests and using specific antisera directed against membrane antigens. In addition, leukaemic blasts of all patients were investigated according to morphological and cytochemical criteria. In APh and ANAE, indices and the percentages of cases showing a granular staining pattern were high in pre‐T‐ and in T‐ALL, but low in C/T‐ and in C‐ALL. PAS‐staining, conversely, was more pronounced in C/T‐ and C‐ALL. APh proved to be more discriminative for recognition of the T‐ and pre‐T‐ALL subgroups than ANAE, but ANAE‐cytochemistry may be useful to detect contaminating normal T‐lymphocytes in ALL. Receptors for C3 were more frequent in C‐ and in T‐ALL than in C/T‐ and in pre‐T‐ALL, receptors for Fc were distributed equally among all subtypes. Positivity of C3‐ and Fc‐receptors was not correlated with cytochemical results. Morphological criteria were not sufficient for subclassification of ALL; the combination of APh‐ and PAS‐staining, however, is valuable to differentiate between C‐subgroups and T‐subgroups.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Cytochemistry and Membrane Markers in Acute Lymphatic Leukaemia (ALL)

Huhn¹,

Thiel

Rodt

et al. 1981

Scandinavian Journal of Haematology

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“…Peroxidaseaktivität wurde in für die elektronenmikroskopische Untersuchung präparierten Zellsuspensioncn mit Diaminobenzidin und H20 2 nachgewiesen [13].…”

Section: Myeloperoxidase1 Ist Ein Lysosomales Enzym Der Neutrophilenunclassified

Familiärer Myeloperoxidasedefekt und akute myeloische Leukämie

Hunh¹,

Bh²,

Haas³

1978

Acta Haematol

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A complete lack of myeloperoxidase (MPO) was demonstrated in a boy suffering from acute myeloic leukemia during the acute phase of the disease and after a remission was achieved. A partial defect of MPO was demonstrated in the patient’s father, no further abnormalities were seen in other members of the family. The fine structure of the patient’s neutrophils and monocytes appeared normal, no activity of MPO was demonstrated on the fine structural level. In the father’s neutrophils transitional forms between cells exhibiting a normal MPO activity and those without activity were demonstrated. The neutrophil bactericidal activity was strongly inhibited in the patient and decreased in his father. Normal values were found in: NBT test, chemotaxis, serum-dependent phagocytosis, number of B and T lymphocytes, serum immunoglobulins, and complement. A possible connection between MPO deficiency and leukemia is discussed.

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Akute Leukämie (aL)

Rastetter¹

1978

Blut Und Blutkrankheiten

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Licht- und elektronenmikroskopische Cytochemie der unreifzelligen Leuk�mien

Cited by 10 publications

References 32 publications

Cytochemistry and Membrane Markers in Acute Lymphatic Leukaemia (ALL)

Cytochemistry and Membrane Markers in Acute Lymphatic Leukaemia (ALL)

Familiärer Myeloperoxidasedefekt und akute myeloische Leukämie

Akute Leukämie (aL)

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