Licochalcone B Extracted from Glycyrrhiza uralensis Fisch Induces Apoptotic Effects in Human Hepatoma Cell HepG2

Abstract

The present study explored the molecular mechanism by which licochalcone B induces the cell cycle arrest and apoptosis in human hepatoma cell HepG2. Initial extraction and identification were performed by HPLC, UPLC-TOF-MS/MS, and NMR analysis, respectively. Licochalcone B inhibited the HepG2 growth with IC 50 (110.15 μM) after 24 h, caused morphological distortion, and seized the cell cycle in the G2/M phase (cell arrest in G2/M:43.1 ± 2.2% for 120 μM versus 23.7 ± 1.2% for control), as well as induced apoptosis and intracellular ROS generation. Furthermore, exposure to licochalcone B markedly affected the cell cycle (up/down regulation) at mRNA and protein levels. Apoptosis was induced through the activation of receptor-mediated and mitochondrial pathways. The inhibition of Caspase 8 and Caspase 9 proteins abolished the licochalcone B induced apoptosis. The present work suggested that licochalcone B may further be identified as a potent functional food component with specific health benefits.

Physicochemical and Antioxidant Properties of Gelatin and Gelatin Hydrolysates Obtained from Extrusion-Pretreated Fish (Oreochromis sp.) Scales

“…In aerobic organisms, the formation of free radicals is unavoidable. Normal aerobic metabolism involves the production of superoxide anion ( • O 2 − ) and hydroxyl radical ( • OH), which are unwanted metabolic by-products [ 2 ]. Free radicals are known to react quickly with other groups and substances, resulting in a wide range of deleterious effects on physiological processes and food [ 3 ].…”

<p>P55PIK Regulates P53-Dependent Apoptosis in Cancer Cells by Interacting with P53 DNA-Specific Domain</p>

“…Cell Culture SW480, HeLa, HepG2, HEK293A, and P6C cells were inoculated in DMEM medium supplemented with 10% fetal calf serum (FBS) while H1299 cells were inoculated in RPMI-1640 medium supplemented with 10% FBS, and cultured at 37°C with 5% CO 2 , as described previously. 35…”

Perspectives and controversies regarding the use of natural products for the treatment of lung cancer

“…In addition to triterpenes, about 300 polyphenols, including phenolic acids, flavonoids, flavanes, chalcones, and isoflavones, with a concentration of 1%–5% each, have been isolated from licorice root and licorice extract 37 . Among them, licorice chalcone A (LICA), Licochalcone B (LCB), and Licochalcone D (LCD) have been proven to provide a variety of health benefits, including anticancer effects 38‐41 …”