Urban ramp bridge plays an important role in unblocking urban traffic and improving urban road network structure. Aiming at the limitations of traditional contact sensors in global measurement, this paper adopts non-contact, high frame rate, high-precision monocular high-speed video measurement technology to measure the dynamic response of the ramp bridge model loaded on the shaking table. The feasibility of video measurement in structure measurement is verified by comparing with the results of wire displacement meter, and the overall measurement accuracy is better than 0.1mm. The seismic effects of two different isolation bearings are compared and analyzed, and the test shows that the plate rubber bearing has a better damping effect.