Lidocaine/prilocaine cream (EMLA®) has an antibacterial effect in vitro

Kerényi, Mónika; Batai, R.; Juhász, Vera P.; Bátai, I.

doi:10.1016/j.jhin.2003.09.013

Cited by 14 publications

(8 citation statements)

References 4 publications

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“…A recent in vitro investigation evaluated the risk of bacterial spread when using a contaminated tube of EMLA ® cream to more than one patient 6 . The same rate of bacterial reduction was found at room temperature as we found at 35°C, for which reason room or body temperature does not seem to play a role in the antibacterial properties of EMLA ® .…”

Section: Discussionsupporting

confidence: 82%

“…Initially, the bacteria were cultured overnight as described above. Then, we prepared seven tubes for each isolate, containing 100 mg EMLA ® and 100 μL bacterial suspension (2 × 10 7 CFU/mL) as was performed by Kerenyi et al 6 The tubes were mixed for 1 minute and incubated for 0, 1, 2, 3, 4, 6, or 24 hours at 35°C (streptococci in a CO 2 ‐rich atmosphere). After the incubation time, 1 mL of 0.4% Tween 20 in Mueller–Hinton broth was added to solubilize the cream, and three serial 10‐fold dilutions were prepared and samples of 100 μL of the 10 −3 , 10 −2 , and 10 −1 dilutions were plated onto agar.…”

Section: Methodsmentioning

confidence: 99%

“…EMLA ® contains two anesthetic drugs: lidocaine 2.5% and prilocaine 2.5%. However, to our knowledge, limited information about EMLA ® is currently available and only one previous study has tested the antibacterial effects of EMLA 6 …”

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confidence: 99%

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Antibacterial properties of EMLA^® and lidocaine in wound tissue biopsies for culturing

Berg

Mössner

Skov

et al. 2006

Wound Repair Regeneration

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If a tissue biopsy from a chronic wound is sampled for culture, the antibacterial properties of local anesthetics may pose a problem in producing false-negative results. The purpose of this study was to investigate the effects of EMLA (AstraZeneca) and lidocaine on common wound pathogenic bacteria. An in vitro study of a total of 25 clinical isolates and ATCC reference strains of Staphylococcus aureus (including methicillin-resistant S. aureus), Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pyogenes was conducted. The isolates were exposed to the local anesthetic drugs (and some of their contents separately) at 35 degrees C over a 24-hour period and time-kill curves were recorded. No culture media were used and saline was used for controls. EMLA was found to have a rapid acting and powerful antibacterial effect and should not be used before culturing tissue samples. Lidocaine 1% was found not to inhibit the bacterial strains when exposure time was held below 2 hours. We conclude that culturing tissue from a wound biopsy is safe within 2 hours when a pure, preservative-free lidocaine 1% solution is used.

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Section: Discussionsupporting

confidence: 82%

Section: Methodsmentioning

confidence: 99%

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Antibacterial properties of EMLA^® and lidocaine in wound tissue biopsies for culturing

Berg

Mössner

Skov

et al. 2006

Wound Repair Regeneration

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“…Lidocaine has been reported to possess antibacterial activity, as in our study. [12][13][14][15][16][17][18] Our aim was not to show the possible use of these preparations as antibiotics but, rather, to investigate whether they had any antibacterial effects and if so, to emphasize that these preparations may provide protective effects in pathology for which they are used as antihistamines. Topical and systemic antihistaminic preparations are used in allergic reactions such as urticaria, sunburn, and bites.…”

Section: Discussionmentioning

confidence: 99%

In vitro antibacterial activity of some systemic and topical antihistaminic preparations

Göçmen

Büyükkoçak²,

Çağlayan³

2009

CIM

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Purpose: In vitro antibacterial activity of topical and systemic antihistaminic preparations containing different active substrates against the standard strains of two bacteria was evaluated. Methods: Four topical and 3 systemic preparations containing pheniramine maleate, chlorophenoxamine hydrochloride, and diphenhydramine hydrochloride were studied. The antibacterial activities of these preparations against strains of S. aureus (American Type Culture Collection, ATCC 29213) and S. epidermidis (ATCC 25212) were tested using the disc diffusion method. In addition, the Minimal Innhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of parenteral preparations for these two bacteria were determined. Results: Pheniramine maleate-topical and pheniramine maleate-systemic had no activity against bacteria, but the others showed various rates of activity. Chlorophenoxamine hydrochloride-topical and chlorophenoxamine hydrochloride-systemic were the most effective (P < 0.05). Despite the same active substrate content, diphenhydramine hydrochloride-topical-1 and diphenhydramine hydrochloride-topical-2 yielded different results when they were compared with each other or with the other preparations. Diphenhydramine hydrochloride-topical-2 had a relatively higher rate of activity than diphenhydramine hydrochloride-topical-1. Inhibition zone diameters were 16.9±1.5 mm 12.3±0.5 mm for S .aureus, 17.4±1.0 mm 0 mm for S .epidermidis respectively (P < 0.05). MIC values of parenteral preparations were equal to or above 125 ?g/ml. Conclusion:, MIC values of parenteral preparations were higher than their blood levels in clinical use. Thus, effects of parenteral preparations may not have been reflected in routine clinical practice. However, topical forms have antibacterial activity due to additive substrates and the use of high concentration levels at the site of application. Therefore, in selection of topical forms for appropriate cases, these effects should also be taken into consideration. The antibacterial activity of topical antihistaminic preparations may be useful in certain dermatological pathology.

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“…As well as their topical anaesthetic properties, lidocaine and prilocaine also possess inherent anti-inflammatory and anti-bacterial actions [38][39][40] , and although these properties have not yet been examined in the TEMPE formulation, might offer additional advantages and provides an interesting area for future research.…”

Section: Tempe Formulation Provides Optimum Residency and Speed Of Onmentioning

confidence: 99%

TEMPE: Topical Eutectic-Like Mixture for Premature Ejaculation

Henry¹,

Morales²,

Wyllie³

2008

Expert Opinion on Drug Delivery

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Lidocaine/prilocaine cream (EMLA®) has an antibacterial effect in vitro

Cited by 14 publications

References 4 publications

Antibacterial properties of EMLA^® and lidocaine in wound tissue biopsies for culturing

Antibacterial properties of EMLA^® and lidocaine in wound tissue biopsies for culturing

In vitro antibacterial activity of some systemic and topical antihistaminic preparations

TEMPE: Topical Eutectic-Like Mixture for Premature Ejaculation

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Lidocaine/prilocaine cream (EMLA®) has an antibacterial effect in vitro

Cited by 14 publications

References 4 publications

Antibacterial properties of EMLA® and lidocaine in wound tissue biopsies for culturing

Antibacterial properties of EMLA® and lidocaine in wound tissue biopsies for culturing

In vitro antibacterial activity of some systemic and topical antihistaminic preparations

TEMPE: Topical Eutectic-Like Mixture for Premature Ejaculation

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Product

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Antibacterial properties of EMLA^® and lidocaine in wound tissue biopsies for culturing

Antibacterial properties of EMLA^® and lidocaine in wound tissue biopsies for culturing