Lidocaine promotes fibroblast proliferation after thermal injury via up‐regulating the expression of miR‐663 and miR‐486

Abstract

Thermal burn is a complex injury that induces pronounced inflammatory reactions and destruction of the microvasculature. Lidocaine, which is broadly used for pain relief, has been reported have the capacity to modulate wound healing processes. Seventeen burn injury patients with no treatment and nine controls were included in this study. The expression of 15 candidate miRNAs in the dermis samples were detected by qRT‐PCR. The target genes were predicted using online bioinformatics tools and confirmed by dual luciferase assay and immunoblotting. The function of miR‐486 and miR‐663 on skin fibroblasts keratinocytes were determined by MTT assay and flow cytometry. The level of miR‐486 and miR‐663 was increased after burn injury, meanwhile, the highest level of miR‐486 and miR‐663 was found after lidocaine treatment. We identified that BCL2L14 was a direct target of both miR‐486 and miR‐663. Meanwhile, overexpression of miR‐486 or miR‐663 inhibit apoptosis and promoted proliferation of human skin fibroblasts keratinocytes. These results indicated that lidocaine treatment promoted the skin healing after thermal injury through up‐regulating miR‐486 and miR‐663 expression, and partially explained how lidocaine modulates wound healing processes. This may provide an evidence for the therapeutic effect of lidocaine during skin healing process. However, the underlying mechanisms need to be further unveiled.

Effect of Stellate Ganglion Block Combined with Lidocaine at Different Concentrations for Preemptive Analgesia on Postoperative Pain Relief and Adverse Reactions of Patients Undergoing Laparoscopic Cholecystectomy

“…Cholecystitis triggers symptoms of nausea and epigastric pain, while gallstones trigger symptoms such as biliary colic and vomiting. Relevant published works have pointed out a higher incidence in women than in men and a higher incidence at older ages [ 2 ]. Naoko et al [ 3 ] et al stated that the incidence of gallstones in the population was about 6.59%, and the incidence over the age of 40 was about 10.15%.…”

MicroRNAs involved in human skin burns, wound healing and scarring

“…[15][16][17] This review will only include previous human research. Samples used to study burn-related miRNAs include adult skin fibroblasts, [18][19][20][21] skin epidermal stem cells, 22 skin, [23][24][25][26][27] wound tissues 28,29 and hypertrophic scar (HS) tissues. [30][31][32][33] Samples used to study wound-healing related miRNAs include healthy skin, [34][35][36] chronic non-healing ulcer tissue, 37,38 epidermal keratinocytes 39 and the HaCaT cell line.…”

“…Other techniques include microarrays (miRCURY LNA, Affymetrix GeneChip, and Agilent) and high throughput sequencing. Although qRT-PCR is the most commonly used technique, only 58 miRNAs were identified in the 27 articles that utilised the technique, [18][19][20][21][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][45][46][47][48][49][50] while the expression level of 82 miRNAs were measured by sequencing in two research studies. 22,38 In comparison, alterations in the expression of 203 miRNAs were detected using three different microarrays in seven research studies.…”

“…Song, 2016 89 hsa-miR-663 BCL2L14 Wu, 2020 25 hsa-miR-1275 HAND2-ASI Yang, 2019 90 as a gold standard in gene expression quantitation. 51 However, it is hindered by various inhibitors, melting temperatures and coexpressed miRNA family members with similar sequences.…”

Helyi érzéstelenítők antibakteriális hatása

“…Korábban leírták, hogy a lidokain és a prilokain gátolhatja az emberi fibroblastok, endothelsejtek és keratinocyták proliferációját in vitro. A legújabb tanulmány szerint a lidokain felületi alkalmazása egyértelműen javította a sebgyógyulást [48].…”